Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) CD-RCd/-
Location 2543142..2543344 Replicon chromosome
Accession NZ_CP011847
Organism Clostridioides difficile strain DSM 27639

Toxin (Protein)


Gene name CD2299.1 Uniprot ID Q185H1
Locus tag CDIF27639_RS12245 Protein ID WP_004454589.1
Coordinates 2543192..2543344 (-) Length 51 a.a.

Antitoxin (RNA)


Gene name SQ1641
Locus tag -
Coordinates 2543142..2543271 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDIF27639_RS12215 2538576..2539040 + 465 WP_011861514.1 hypothetical protein -
CDIF27639_RS12225 2539368..2539529 - 162 WP_004454578.1 hypothetical protein -
CDIF27639_RS12230 2539581..2540394 - 814 Protein_2257 toxin Bro -
CDIF27639_RS20200 2540514..2540666 - 153 WP_021361915.1 hypothetical protein -
CDIF27639_RS12240 2542075..2542998 - 924 WP_011861517.1 SHOCT domain-containing protein -
- 2543142..2543271 + 130 NuclAT_3 - Antitoxin
CDIF27639_RS12245 2543192..2543344 - 153 WP_004454589.1 hypothetical protein Toxin
CDIF27639_RS12250 2543618..2543941 - 324 WP_009897482.1 hypothetical protein -
CDIF27639_RS12255 2543977..2544231 - 255 WP_004454592.1 hypothetical protein -
CDIF27639_RS12265 2544667..2545185 - 519 Protein_2263 transposase -
CDIF27639_RS12270 2545475..2545850 - 376 Protein_2264 BlaI/MecI/CopY family transcriptional regulator -
CDIF27639_RS12275 2545955..2546332 - 378 WP_011861518.1 BlaI/MecI/CopY family transcriptional regulator -
CDIF27639_RS12280 2547136..2547630 + 495 WP_011861519.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
CDIF27639_RS12285 2548019..2548189 + 171 WP_004454601.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2536302..2553438 17136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.



Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.92 Da        Isoelectric Point: 10.6365

>T55097 WP_004454589.1 NZ_CP011847:c2543344-2543192 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Download         Length: 153 bp

>T55097 NZ_CP011847:c2543344-2543192 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin


Download         Length: 130 bp

>AT55097 NZ_CP011847:2543142-2543271 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A125V725

Antitoxin

Download structure file

References