23246

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 440568..441186 Replicon chromosome
Accession NC_011601
Organism Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag E2348C_RS02070 Protein ID WP_001291435.1
Coordinates 440568..440786 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag E2348C_RS02075 Protein ID WP_000344800.1
Coordinates 440812..441186 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2348C_RS02045 (E2348_C_0390) 436457..436768 - 312 WP_000409907.1 MGMT family protein -
E2348C_RS02050 (E2348_C_0391) 437147..437500 + 354 WP_000878140.1 DUF1428 domain-containing protein -
E2348C_RS02055 (E2348_C_0392) 437542..439092 - 1551 WP_001386100.1 cyclic-guanylate-specific phosphodiesterase PdeB -
E2348C_RS02060 (E2348_C_0393) 439256..439726 - 471 WP_000136192.1 YlaC family protein -
E2348C_RS02065 (E2348_C_0394) 439842..440394 - 553 Protein_397 maltose O-acetyltransferase -
E2348C_RS02070 (E2348_C_0395) 440568..440786 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
E2348C_RS02075 (E2348_C_0396) 440812..441186 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
E2348C_RS02080 (E2348_C_0397) 441731..444880 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
E2348C_RS02085 (E2348_C_0398) 444903..446096 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 435058..436431 1373


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T23246 WP_001291435.1 NC_011601:c440786-440568 [Escherichia coli O127:H6 str. E2348/69]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T23246 NC_011601:c440786-440568 [Escherichia coli O127:H6 str. E2348/69]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT23246 WP_000344800.1 NC_011601:c441186-440812 [Escherichia coli O127:H6 str. E2348/69]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT23246 NC_011601:c441186-440812 [Escherichia coli O127:H6 str. E2348/69]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References