Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) symER/SymE(toxin)
Location 728315..728724 Replicon chromosome
Accession NZ_CP028754
Organism Escherichia coli strain A21

Toxin (Protein)


Gene name symE Uniprot ID A0A3L4RJH9
Locus tag AOY74_RS03350 Protein ID WP_001357525.1
Coordinates 728315..728653 (-) Length 113 a.a.

Antitoxin (RNA)


Gene name symR
Locus tag -
Coordinates 728648..728724 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY74_RS03325 (723682) 723682..724962 - 1281 WP_001523887.1 DUF445 domain-containing protein -
AOY74_RS03330 (725147) 725147..726067 + 921 WP_022296911.1 Rpn family recombination-promoting nuclease/putative transposase -
AOY74_RS03335 (726059) 726059..726373 - 315 Protein_633 winged helix-turn-helix domain-containing protein -
AOY74_RS03340 (726551) 726551..726715 + 165 WP_001357524.1 DUF1127 domain-containing protein -
AOY74_RS03345 (726750) 726750..728123 - 1374 WP_000244358.1 IS4-like element ISEc13 family transposase -
AOY74_RS03350 (728315) 728315..728653 - 339 WP_001357525.1 endoribonuclease SymE Toxin
- (728648) 728648..728724 + 77 NuclAT_13 - Antitoxin
- (728648) 728648..728724 + 77 NuclAT_13 - Antitoxin
- (728648) 728648..728724 + 77 NuclAT_13 - Antitoxin
- (728648) 728648..728724 + 77 NuclAT_13 - Antitoxin
- (728648) 728648..728724 + 77 NuclAT_14 - Antitoxin
- (728648) 728648..728724 + 77 NuclAT_14 - Antitoxin
- (728648) 728648..728724 + 77 NuclAT_14 - Antitoxin
- (728648) 728648..728724 + 77 NuclAT_14 - Antitoxin
AOY74_RS03355 (728868) 728868..729077 - 210 Protein_637 DUF3387 domain-containing protein -
AOY74_RS03360 (729306) 729306..731156 - 1851 WP_000190966.1 nuclease-related domain-containing DEAD/DEAH box helicase -
AOY74_RS03365 (731487) 731487..732443 - 957 WP_022296912.1 GTPase -
AOY74_RS03370 (732454) 732454..732657 - 204 WP_000467859.1 YbdD/YjiX family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 726750..728123 1373


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SymE-like

(20-71)


Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 12314.03 Da        Isoelectric Point: 7.8234

>T103630 WP_001357525.1 NZ_CP028754:c728653-728315 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFTTGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQRQVQDFIGVISSKTPR

Download         Length: 339 bp

>T103630 NZ_CP028754:c728653-728315 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGCCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTACCA
CCGGCACGGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCACTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGATTTTATTGGGGTGATTAG
TAGCAAAACGCCACGCTAA

Antitoxin


Download         Length: 77 bp

>AT103630 NZ_CP028754:728648-728724 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3L4RJH9

Antitoxin

Download structure file

References