220306

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yjhX-yjhQ/YjhX-GNAT
Location 2514617..2515431 Replicon chromosome
Accession NZ_CP085063
Organism Escherichia coli strain P31_YH1_02.21

Toxin (Protein)


Gene name yjhX Uniprot ID S1PA82
Locus tag LH370_RS12220 Protein ID WP_001054376.1
Coordinates 2514617..2514874 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name yjhQ Uniprot ID U9Z4B8
Locus tag LH370_RS12225 Protein ID WP_001309181.1
Coordinates 2514886..2515431 (+) Length 182 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH370_RS12195 (2509905) 2509905..2511011 + 1107 WP_001309184.1 N-acetylneuraminate epimerase -
LH370_RS12200 (2511076) 2511076..2512056 + 981 WP_000991438.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
LH370_RS12205 (2512166) 2512166..2512371 + 206 Protein_2387 HNH endonuclease -
LH370_RS12210 (2512639) 2512639..2513879 - 1241 Protein_2388 helicase YjhR -
LH370_RS12215 (2513995) 2513995..2514126 + 132 WP_001309182.1 hypothetical protein -
LH370_RS12220 (2514617) 2514617..2514874 + 258 WP_001054376.1 YjhX family toxin Toxin
LH370_RS12225 (2514886) 2514886..2515431 + 546 WP_001309181.1 N-acetyltransferase Antitoxin
LH370_RS12230 (2515487) 2515487..2516233 + 747 WP_000354251.1 class I SAM-dependent methyltransferase -
LH370_RS12235 (2516402) 2516402..2516620 + 219 Protein_2393 hypothetical protein -
LH370_RS12240 (2516658) 2516658..2516774 + 117 Protein_2394 VOC family protein -
LH370_RS12245 (2517019) 2517019..2518140 + 1122 WP_000010829.1 M42 family metallopeptidase -
LH370_RS12250 (2518137) 2518137..2518415 + 279 WP_000722973.1 PTS sugar transporter subunit IIB SgcB -
LH370_RS12255 (2518427) 2518427..2519740 + 1314 WP_000460843.1 PTS sugar transporter subunit IIC SgcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - fimB 2507111..2515431 8320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Putative toxin of bacterial toxin-antitoxin pair

(1-85)

Antitoxin

GNAT domain

(18-121)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 9734.29 Da        Isoelectric Point: 11.0090

>T220306 WP_001054376.1 NZ_CP085063:2514617-2514874 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Download         Length: 258 bp

>T220306 NZ_CP117006:219672-219779 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 182 a.a.        Molecular weight: 19956.90 Da        Isoelectric Point: 6.3277

>AT220306 WP_001309181.1 NZ_CP085063:2514886-2515431 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Download         Length: 546 bp

>AT220306 NZ_CP117006:c219623-219560 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PA82

Antitoxin

Source ID Structure
AlphaFold DB U9Z4B8

References