T4SS ID	7
Strain	Agrobacterium tumefaciens plasmid Ti (NC_002377)
Replicon	plasmid Ti [Browse all T4SS(s) in this replicon]
Accession	NC_002377
Location	151232..172619
Name	VirB
Function	effector translocation and conjugation
Classification	Type IVA; Type P
Experimental investigation has been performed on this T4SS.

T4SS components

Component	VirB1	VirB2	VirB3	VirB4	VirB5	VirB6	VirB7	VirB8	VirB9
Number	1	1	1	1	1	1	1	1	1
Component	VirB10	VirB11	VirD4
Number	1	1	1

The information of T4SS components from NC_002377
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	pTi_126 (virJ)	148762..149529 [+], 768	10955142	hypothetical protein
2	pTi_127 (virB1)	151232..151951 [+], 720	10955143	type IV secretion system lytic transglycosylase VirB1	VirB1
3	pTi_128 (virB2)	151966..152331 [+], 366	10955144	type IV secretion system pilin subunit VirB2	VirB2
4	pTi_129 (virB3)	152331..152657 [+], 327	10955145	type IV secretion system protein VirB3	VirB3
5	pTi_130 (virB4)	152657..155026 [+], 2370	10955146	type IV secretion system protein VirB4	VirB4
6	pTi_131 (virB5)	155041..155703 [+], 663	10955147	type IV secretion system protein VirB5	VirB5
7	pTi_132 (virB6)	155800..156687 [+], 888	10955148	type IV secretion system protein VirB6	VirB6
8	pTi_133 (virB7)	156721..156888 [+], 168	10955149	type IV secretion system lipoprotein VirB7	VirB7
9	pTi_134 (virB8)	156896..157588 [+], 693	10955150	type IV secretion system protein VirB8	VirB8
10	pTi_135 (virB9)	157585..158466 [+], 882	10955151	type IV secretion system protein VirB9	VirB9
11	pTi_136 (virB10)	158463..159596 [+], 1134	10955152	type IV secretion system protein VirB10	VirB10
12	pTi_137 (virB11)	159636..160667 [+], 1032	10955153	type IV secretion system protein VirB11	VirB11
13	pTi_138 (virG)	160877..161602 [+], 726	10955154	two-component response regulator VirG
14	pTi_139 (virC2)	166669..167277 [-], 609	10955155	putative crown gall tumor protein VirC2
15	pTi_140 (virC1)	167280..167975 [-], 696	10955156	putative crown gall tumor protein VirC1
16	pTi_141 (virD1)	168244..168687 [+], 444	10955157	type IV secretion system T-DNA border endonuclease VirD1
17	pTi_142 (virD2)	168721..169995 [+], 1275	10955158	type IV secretion system T-DNA border endonuclease VirD2
18	pTi_143 (virD3)	170015..170620 [+], 606	10955159	hypothetical protein
19	pTi_144 (virD4)	170649..172619 [+], 1971	10955160	type IV secretion system protein VirD4	VirD4
20	pTi_145 (virD5)	172711..175212 [+], 2502	10955161	hypothetical protein
21	pTi_146 (virE1)	175665..175862 [+], 198	10955162	type IV secretion system chaperone VirE1
22	pTi_147 (virE2)	175867..177468 [+], 1602	10955163	type IV secretion system single-stranded DNA binding protein VirE2

 This T4SS contains information of accessory protein.

Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files

Proteins        Genes

This system delivers both DNA and proteins into plant cell. The transferred DNA (T-DNA) region: NC_002377: 1..22878)

Effectors

VirD2, VirD5, VirE2, VirE3, VirF

The information of protein effectors
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	*
1	pTi_142 (virD2)	168721..169995 [+], 1275	10955158	type IV secretion system T-DNA border endonuclease VirD2	VirD2
2	pTi_145 (virD5)	172711..175212 [+], 2502	10955161	hypothetical protein	VirD5
3	pTi_147 (virE2)	175867..177468 [+], 1602	10955163	type IV secretion system single-stranded DNA binding protein VirE2	VirE2
4	pTi_148 (virE3)	177534..179552 [+], 2019	10955164	hypothetical protein	VirE3
5	pTi_152 (virF)	184764..185372 [+], 609	10955168	hypothetical protein	VirF

 T4SE derived from experimental literature.

Download FASTA format files

Proteins        Genes

The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#	Accessory protein(GI)	motif(s)	Substrate(s)	Function	Reference
1	VirC1 (10955156)	ND	VirD2	VirC1 pairwise interacts with the processing factors VirD2 relaxase, VirC2, and VirD1 to stimulate generation of multiple copies per cell of the T-DNA substrate. VirC1 promotes conjugative T-DNA transfer.	(1) PubMed: 17505518
2	VirE1 chaperone (10955162)	CT50aa sufficient (interacts with the VirD4 T4CP. CT100aa(VirE2) sufficient for the T4CP interation)	VirE2	Translocation of the VirE2 effector through the A. tumefaciens VirB/VirD4 T4SS requires VirE1.	(2) PubMed: 19946141

Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Atmakuri K; Cascales E; Burton OT; Banta LM; Christie PJ (2007). Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions. EMBO J. 26(10):2540-51. [PudMed:17505518]

(2) Alvarez-Martinez CE; Christie PJ (2009). Biological diversity of prokaryotic type IV secretion systems. Microbiol Mol Biol Rev. 73(4):775-808. [PudMed:19946141]

(1) Ream W (2009). Agrobacterium tumefaciens and A. rhizogenes use different proteins to transport bacterial DNA into the plant cell nucleus. Microb Biotechnol. 2(4):416-27. [PudMed:21255274]