| Effector Protein ID: 10955158 |
 T4SS name [ID] | VirB [7] |
| Gene symbol | virD2 |
| Locus tag | pTi_142 |
| Strain | Agrobacterium tumefaciens plasmid Ti (NC_002377) |
| Replicon | plasmid Ti [GenBank: NC_002377] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 168721..169995 [+] |
| Product | type IV secretion system T-DNA border endonuclease VirD2 |
| Effector type | Protein |
| Descripton | VirD2 |
| T4CP requirement | T4CP Depedent |
| Target/Effection | VirD2 is an endonuclease that nicks the T-DNA region of Ti-/Ri-plasmids at border repeat sequences flanking T-DNA. During T-DNA processing, VirD2 covalently attaches to the 5′ end of the resulting T-strand. VirD2 serves as a “pilot protein” to lead T-strands through the T4SS and into the host cell. Once in the host, VirD2 probably plays a major role in targeting T-strands to the nucleus |
| UniProt ID | Q79AU0, P06668 |
| KEGG ID | NA |
| PDB ID | NA |
| Domain hit(s) vs Pfam | Relaxase [PF03432], Evalue: 3.8e-51, Aligned region: 16..263 |
| Protein Sequence: 424 a.a. [Download] |
| >gi|10955158|ref|NP_059814.1| hypothetical protein pTi_142 [Agrobacterium tumefaciens] MPDRAQVIIRIVPGGGTKTLQQIINQLEYLSRKGKLELQRSARHLDIPVPPDQIRELAQSWVTEAGIYDE SQSDDDRQQDLTTHIIVSFPAGTDQTAAYEASREWAAEMFGSGYGGGRYNYLTAYHVDRDHPHLHVVVNR RELLGHGWLKISRRHPQLNYDGLRKKMAEISLRHGIVLDATSRAERGIAERPITYAEHRRLERMQAQKIQ FEDTDFDETSPEEDRRDLSQSFDPFRSDPSTGEPDRATRHDKQPLEQHARFQESAGSSIKADARIRVSLE SERSAQPSASKIPVIGHFGIETSYVAEASVRKRSGIFGTSRPVTDVAMHTVKRQQRSKRRNDEEAGPSGA NRKGLKAAQVDSEANVGEQDTRDDSNKAADPVSASIGTEQPEASPKRPRDRHDGELGGRKRARGNRRDDG RGGT |
| - |
| Nucleotide Sequence: 1275 bp [Download] |
| >gi|10955016|ref|NC_002377.1|:168721-169995 Agrobacterium tumefaciens plasmid Ti, complete sequence ATGCCCGATCGCGCTCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAAGACCCTTCAGCAGATAA TCAATCAGTTGGAGTACCTGTCCCGTAAGGGAAAGCTGGAACTGCAGCGTTCAGCCCGGCATCTCGATAT TCCCGTTCCGCCGGATCAAATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTATGACGAA AGTCAGTCAGACGATGATAGGCAACAAGACTTAACAACACACATTATTGTAAGCTTCCCCGCAGGTACCG ACCAAACCGCAGCTTATGAAGCCAGCCGGGAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGG CCGCTATAACTATCTGACAGCCTACCACGTCGACCGCGATCATCCACATTTACATGTCGTGGTCAATCGT CGGGAACTTCTGGGGCACGGGTGGCTGAAAATATCCAGGCGCCATCCCCAGCTGAATTATGACGGCTTAC GGAAAAAGATGGCAGAGATTTCACTTCGTCACGGCATAGTCCTGGATGCGACTTCGCGAGCAGAAAGGGG AATAGCAGAGCGACCAATCACATATGCTGAACATCGCCGCCTTGAGCGGATGCAGGCTCAAAAGATTCAA TTCGAAGATACAGATTTTGATGAGACCTCGCCTGAGGAAGATCGTCGGGACCTCAGTCAATCGTTCGATC CATTTCGATCGGACCCATCTACCGGCGAACCGGACCGTGCAACCCGACATGACAAACAACCGCTTGAACA GCACGCCCGTTTCCAGGAGTCCGCCGGCTCCAGCATCAAAGCCGACGCACGGATCCGCGTATCATTGGAG AGCGAGCGGAGTGCCCAACCATCCGCGTCCAAAATCCCTGTAATTGGGCATTTCGGGATTGAGACTTCCT ATGTCGCTGAAGCCAGCGTGCGCAAACGAAGCGGCATTTTCGGTACTTCTCGCCCGGTGACTGACGTTGC CATGCACACAGTCAAGCGCCAGCAGCGATCAAAACGACGTAATGACGAGGAGGCAGGTCCGAGCGGAGCA AACCGTAAAGGATTGAAGGCTGCGCAAGTTGATTCCGAGGCAAATGTCGGTGAGCAAGACACTCGCGATG ACAGCAACAAGGCGGCTGATCCGGTGTCTGCTTCCATCGGTACCGAGCAACCGGAAGCTTCTCCAAAGCG TCCGCGTGACCGTCACGATGGAGAATTGGGTGGACGCAAACGTGCAAGAGGTAATCGTCGCGACGATGGG CGCGGGGGGACCTAG |
| Reference |
| (1) Ream W (2009). Agrobacterium tumefaciens and A. rhizogenes use different proteins to transport bacterial DNA into the plant cell nucleus. Microb Biotechnol. 2(4):416-27. [PudMed:21255274] |