T4SS ID493
StrainEhrlichia chaffeensis str. Arkansas
Repliconchromosome [Browse all T4SS(s) in this replicon]
Location36100..42178; 206250..207062; 481051..501957; 1071112..1077767
Functioneffector translocation
ClassificationType IVA; Type P
experimental Experimental investigation has been performed on this T4SS.

T4SS components

The information of T4SS components from NC_007799
Region 1: 36100..42178
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1ECH_0036 (nadA)31156..32100 [+], 94588657840quinolinate synthetase 
2ECH_0038 (fdxA)32814..33191 [-], 37888658543ferredoxin A 
3ECH_003933455..35101 [+], 164788658043120 kDa immunodominant surface protein 
4ECH_0040 (virD4)36100..38244 [-], 214588657566type IV secretion system component VirD4  VirD4
5ECH_0041 (virB11)38281..39279 [-], 99988658241type IV secretion system ATPase VirB11  VirB11
6ECH_0042 (virB10)39295..40638 [-], 134488658039type IV secretion system protein VirB10  VirB10
7ECH_0043 (virB9-1)40660..41481 [-], 82288658647type IV secretion system protein VirB9  VirB9
8ECH_0044 (virB8-1)41465..42178 [-], 71488657917type IV secretion system protein VirB8  VirB8
9ECH_004542310..43407 [-], 109888657727putative GTP cyclohydrolase II 
10ECH_004743622..43765 [+], 14488657997hypothetical protein 
11ECH_004843952..45022 [-], 107188658481TPR domain-containing protein 
12ECH_004945097..45519 [-], 42388657849hypothetical protein 
13ECH_0050 (dapF)45664..46467 [-], 80488658257diaminopimelate epimerase 
14ECH_005146685..46840 [+], 15688658485hypothetical protein 
15ECH_005246729..47151 [-], 42388658254hypothetical protein 
Region 2: 206250..207062
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1ECH_0212201489..202403 [-], 91588658381putative transporter 
2ECH_0213202899..203213 [-], 31588657857putative oxidoreductase 
3ECH_0214203259..203447 [+], 18988658528putative exodeoxyribonuclease VII, small subunit 
4ECH_0215203618..203830 [+], 21388658040hypothetical protein 
5ECH_0216203787..203897 [+], 11188658643hypothetical protein 
6ECH_0217204031..205269 [+], 123988658127MiaB family tRNA modification protein 
7ECH_0218 (trx)205658..205981 [-], 32488658653thioredoxin 
8ECH_0219 (virB9-2)206250..207062 [-], 81388658361type IV secretion system protein, VirB9  VirB9
9ECH_0220 (pdhA)207354..208337 [-], 98488657641pyruvate dehydrogenase complex, E1 component, pyruvate dehydrogenase alpha subunit 
10ECH_0221208547..209401 [-], 85588658364putative hydrolase 
Region 3: 481051..501957
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1ECH_0490 (lipA)477049..477942 [+], 89488657773lipoyl synthase 
2ECH_0492478543..479811 [-], 126988657651putative phosphate ABC transporter, permease protein 
3ECH_0493 (sodB)480037..480654 [+], 61888658263superoxide dismutase, Fe 
4ECH_0494 (virB3)481051..481344 [+], 29488657764type IV secretion system protein VirB3  VirB3
5ECH_0495 (virB4-1)481344..483746 [+], 240388657911type IV secretion system ATPase VirB4  VirB4
6ECH_0496 (virB6)483786..486266 [+], 248188658009type IV secretion system protein VirB6  VirB6
7ECH_0497486464..489232 [+], 276988658206VirB6 family type IV secretion system protein  VirB6
8ECH_0498489236..493642 [+], 440788657563VirB6 family type IV secretion system protein  VirB6
9ECH_0499493681..501957 [+], 827788658162VirB6 family type IV secretion system protein  VirB6
10ECH_0500502109..502708 [-], 60088657625hypothetical protein 
11ECH_0501 (dut)502914..503381 [-], 46888657998deoxyuridine 5'triphosphate nucleotidohydrolase 
12ECH_0502 (ispH)503528..504487 [-], 960886586114-hydroxy-3-methylbut-2-enyl diphosphate reductase 
13ECH_0503 (carA)505048..506163 [-], 111688658507carbamoyl phosphate synthase small subunit 
Region 4: 1071112..1077767
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1ECH_10391070664..1070759 [-], 9688657597hypothetical protein 
2ECH_10401070866..1071039 [-], 17488658429hypothetical protein 
3ECH_1041 (virB4-2)1071112..1073487 [-], 237688657785type IV secretion system protein VirB4  VirB4
4ECH_10421074410..1074784 [-], 37588657979hypothetical protein  VirB2
5ECH_10431074796..1075101 [-], 30688658403hypothetical protein  VirB2
6ECH_10441075174..1075530 [-], 35788658648hypothetical protein  VirB2
7ECH_10451076659..1076835 [-], 17788657992hypothetical protein 
8ECH_10461077077..1077175 [-], 9988657964hypothetical protein 
9ECH_10471077396..1077767 [-], 37288658442hypothetical protein  VirB2
10ECH_10481077826..1078020 [-], 19588657978hypothetical protein 
11ECH_10491078435..1078527 [+], 9388657748hypothetical protein 
12ECH_1050 (hflK)1078544..1079617 [+], 107488657696hflK protein 
13ECH_1051 (hflC)1079622..1080491 [+], 87088658164hflC protein 
14ECH_10521080495..1081910 [+], 141688658017serine protease 
15ECH_10531081912..1082493 [+], 58288658500hypothetical protein 
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes

Etf-1, Etf-3, Etf-2

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1ECH_0825 841411..842553 [+], 114388658118hypothetical proteinexperimental  Etf-1interaction
2ECH_0767 774594..776459 [+], 186688658219hypothetical proteinexperimental  Etf-3
3ECH_0261 244693..245487 [-], 79588658269hypothetical proteinexperimental  Etf-2interaction
experimental T4SE derived from experimental literature.
interaction This effector contains information of interaction.

Download FASTA format files
Proteins        Genes
The information on host-pathogen interaction.

#Name(Protein GI)Host site/SubstrateSourceFunctionReference
1Etf-1 (88658118)Rab5humanEhrlichial translocated factor-1 (Etf-1) induces autophagy which induced by Rab5 to provide E. chaffeensis with host cytosolic nutrients which is required for proliferation of E. chaffeensis.(1) PubMed: 28638803experimental
2Etf-2 (88658269)Rab5humanEtf-2 binds to active RAB5 and disturbs with the engagement of RAB5 with RAB5-specific GAP in order to avoid routing to host-cell phagolysosomes.(2) PubMed: 30181274experimental
experimental This T4SE is mentioned in the literature.

(1) Sharma P et al. (2017). Peptide Nucleic Acid Knockdown and Intra-host Cell Complementation of Ehrlichia Type IV Secretion System Effector. Front Cell Infect Microbiol. 7:228. [PudMed:28638803] experimental
(2) Yan Q et al. (2018). Ehrlichia type IV secretion system effector Etf-2 binds to active RAB5 and delays endosome maturation. Proc Natl Acad Sci U S A. 115(38):E8977-E8986. [PudMed:30181274] experimental
(1) Liu H; Bao W; Lin M; Niu H; Rikihisa Y (2012). Ehrlichia type IV secretion effector ECH0825 is translocated to mitochondria and curbs ROS and apoptosis by upregulating host MnSOD. Cell Microbiol. 14(7):1037-50. [PudMed:22348527] experimental
(2) Bao W; Kumagai Y; Niu H; Yamaguchi M; Miura K; Rikihisa Y (2009). Four VirB6 paralogs and VirB9 are expressed and interact in Ehrlichia chaffeensis-containing vacuoles. J Bacteriol. 191(1):278-86. [PudMed:18952796] experimental
(3) Cheng Z; Wang X; Rikihisa Y (2008). Regulation of type IV secretion apparatus genes during Ehrlichia chaffeensis intracellular development by a previously unidentified protein. J Bacteriol. 190(6):2096-105. [PudMed:18192398] experimental
(4) Dunning Hotopp JC; Lin M; Madupu R; Crabtree J; Angiuoli SV; Eisen JA; Seshadri R; Ren Q; Wu M; Utterback TR; Smith S; Lewis M; Khouri H; Zhang C; Niu H; Lin Q; Ohashi N; Zhi N; Nelson W; Brinkac LM; Dodson RJ; Rosovitz MJ; Sundaram J; Daugherty SC; Davidsen T; Durkin AS; Gwinn M; Haft DH; Selengut JD; Sullivan SA; Zafar N; Zhou L; Benahmed F; Forberger H; Halpin R; Mulligan S; Robinson J; White O; Rikihisa Y; Tettelin H (2006). Comparative genomics of emerging human ehrlichiosis agents. PLoS Genet. 2(2):e21. [PudMed:16482227]
(5) Ohashi N; Zhi N; Lin Q; Rikihisa Y (2002). Characterization and transcriptional analysis of gene clusters for a type IV secretion machinery in human granulocytic and monocytic ehrlichiosis agents. Infect Immun. 70(4):2128-38. [PudMed:11895979] experimental
experimental This literature contains experimental investigation