| Effector Protein ID: 88658118 |
 T4SS name [ID] | VirB [493] |
| Gene symbol | - |
| Locus tag | ECH_0825 |
| Strain | Ehrlichia chaffeensis str. Arkansas |
| Replicon | chromosome [GenBank: NC_007799] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 841411..842553 [+] |
| Product | hypothetical protein |
| Effector type | Protein |
| Descripton | Etf-1 |
| T4CP requirement | T4CP Depedent |
| Target/Effection | ECH0825 prevents ROS-induced cellular damage and apoptosis to allow intracellular infection by upregulating MnSOD (Liu, et al. 2012) Etf-1 interacts with the RAB5-BECN1-PIK3C3 autophagy master regulator complex via GTP-bound RAB5, to induce autophagy. |
| UniProt ID | Q2GG12 |
| KEGG ID | ech:ECH_0825 |
| PDB ID | NA |
| Domain hit(s) vs Pfam | NA |
| Protein Sequence: 380 a.a. [Download] |
| >gi|88658118|ref|YP_507621.1| hypothetical protein ECH_0825 [Ehrlichia chaffeensis str. Arkansas] MLTFLKKGANVVIKAAITPTTSKLPHQESVGKYLGGMLKSIIPGQKDPAEKHFDLRMHNRTHKLGVELPA RTRIDGHEQEVALKVPSYKLPYQTLQKFAAWQEQDKEGEVEETKKDTLTSLINPVSATQEFIRSNTADKY FEKLKALDQQLKEIGGKVTVNSTPGRYKQGLTIELDVSNKSRIAIEKDVRLVLEGLGINDEELVKTFTKR LYKSSTDKKMSSADIHTEKTSSNTDSKDNSNNQDHDIVSPAKRHGSDTVSAVQRQGDDILSSSQEQSSKT GSPTNSTGISNSDFSLTNPQNMPYSRTPMQSSQMLQQLQNQILMQQKPERIFSQAMPTVMQQEIDEIAED LCQGLNEGPTQGNSNNIGKHFSNPGKVHAR |
| - |
| Nucleotide Sequence: 1143 bp [Download] |
| >gi|88657561|ref|NC_007799.1|:841411-842553 Ehrlichia chaffeensis str. Arkansas, complete genome ATGCTTACTTTCTTAAAGAAAGGTGCAAATGTTGTTATAAAAGCAGCTATAACACCCACCACTAGTAAAT TACCCCATCAAGAAAGCGTAGGTAAATACCTTGGAGGCATGCTGAAAAGTATTATTCCAGGTCAAAAAGA TCCTGCTGAGAAACACTTTGACTTAAGAATGCACAACCGTACCCACAAACTGGGGGTAGAATTACCAGCA AGAACAAGAATAGATGGTCATGAGCAAGAAGTAGCTCTGAAAGTACCATCTTATAAATTACCATATCAAA CTTTACAAAAATTCGCAGCATGGCAAGAACAAGATAAAGAAGGAGAGGTAGAAGAAACTAAAAAAGATAC CTTAACATCACTTATTAATCCTGTATCAGCAACTCAAGAGTTCATTAGAAGTAATACTGCTGATAAATAT TTTGAAAAATTAAAAGCACTTGATCAGCAATTGAAAGAAATAGGAGGAAAAGTTACAGTAAATTCTACTC CAGGTAGATATAAACAAGGATTAACTATAGAATTAGATGTTTCCAACAAATCAAGAATAGCAATAGAGAA AGATGTACGACTTGTTCTAGAAGGTTTAGGTATAAATGATGAAGAATTAGTCAAAACTTTTACTAAAAGA CTATATAAATCATCTACTGATAAAAAAATGAGTTCAGCAGATATTCACACTGAGAAAACTAGTTCTAATA CTGATAGCAAAGACAATTCTAATAATCAAGATCATGATATAGTATCACCAGCTAAAAGACATGGAAGTGA TACAGTATCAGCAGTACAAAGGCAAGGAGATGATATACTATCATCATCTCAAGAACAGAGTAGTAAAACT GGATCACCAACTAACAGTACTGGTATATCAAACAGTGATTTTTCACTTACAAATCCTCAAAATATGCCTT ATTCTAGGACCCCTATGCAGAGTTCACAAATGCTACAGCAGTTGCAAAATCAAATATTGATGCAACAAAA ACCTGAAAGGATATTTTCACAGGCTATGCCTACTGTTATGCAACAAGAAATCGATGAAATAGCAGAAGAT TTATGTCAAGGTCTTAATGAAGGGCCAACACAAGGTAATTCAAACAATATAGGAAAGCATTTTAGTAATC CAGGAAAAGTACATGCAAGATAA |
| Reference |
(1) Sharma P et al. (2017). Peptide Nucleic Acid Knockdown and Intra-host Cell Complementation of Ehrlichia Type IV Secretion System Effector. Front Cell Infect Microbiol. 7:228. [PudMed:28638803] 
|
|
(2) Lin M et al. (2016). Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase. Autophagy. 12(11):2145-2166. [PudMed:27541856]
|
|
(3) Liu H; Bao W; Lin M; Niu H; Rikihisa Y (2012). Ehrlichia type IV secretion effector ECH0825 is translocated to mitochondria and curbs ROS and apoptosis by upregulating host MnSOD. Cell Microbiol. 14(7):1037-50. [PudMed:22348527]
|
| experimental literature |