SecReT4
T4SS ID36
StrainHelicobacter pylori Shi470
Repliconchromosome [Browse all T4SS(s) in this replicon]
AccessionNC_010698
Location808395..844300
NameCag
Functioneffector translocation
ClassificationType IVA; Type P
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components
ComponentCagCCagDCagECagFCagGCagHCagICagLCagN
Number211111111
ComponentCagMCagTCagUCagVCagWCagXCagYCagZCagalpha
Number111111111
ComponentCagbetaCaggammaCagdelta
Number111

The information of T4SS components from NC_010698
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1HPSH_04145804109..807594 [-], 3486188527607cag pathogenicity island protein CagA 
2HPSH_04150808001..808228 [+], 228188527608cag pathogenicity island protein CagB 
3HPSH_04155808395..808742 [+], 348188527609cag pathogenicity island protein CagC  CagC
4HPSH_04160808744..809367 [+], 624188527610cag pathogenicity island protein CagD  CagD
5HPSH_04165809382..812327 [+], 2946188527611cag pathogenicity island protein CagE  CagE
6HPSH_04170812329..813135 [+], 807188527612cag pathogenicity island protein CagF  CagFaccesspry protein
7HPSH_04175813190..813621 [+], 432188527613cag pathogenicity island protein CagG  CagG
8HPSH_04180813607..813804 [-], 198188527614hypothetical protein 
9HPSH_04185813803..814735 [+], 933188527615cag pathogenicity island protein CagH  CagH
10HPSH_04190814746..815885 [+], 1140188527616cag pathogenicity island protein CagI  CagI
11HPSH_04195815882..816595 [+], 714188527617cag pathogenicity island protein CagL  CagLinteraction
12HPSH_04200816678..817598 [-], 921188527618cag pathogenicity island protein CagN  CagN
13HPSH_04205817613..818743 [-], 1131188527619cag pathogenicity island protein CagM  CagM
14HPSH_04210819169..819513 [+], 345188527620cag pathogenicity island protein CagP 
15HPSH_04215819912..823169 [-], 3258188527621cag pathogenicity island protein CagA 
16HPSH_04220823362..823460 [+], 99188527622hypothetical protein 
17HPSH_04225823481..823708 [+], 228188527623cag pathogenicity island protein CagB 
18HPSH_04230823876..824067 [+], 192188527624cag pathogenicity island protein CagC  CagC
19HPSH_04235824137..824232 [+], 96188527625hypothetical protein 
20HPSH_04240824858..825163 [-], 306188527626cag pathogenicity island protein CagQ 
21HPSH_04245825192..825374 [-], 183188527627hypothetical protein 
22HPSH_04250825507..825644 [+], 138188527628hypothetical protein 
23HPSH_04255826078..826677 [+], 600188527629cag pathogenicity island protein CagS 
24HPSH_04260826879..827721 [-], 843188527630cag pathogenicity island protein CagT  CagT
25HPSH_04265827737..828384 [-], 648188527631cag pathogenicity island protein CagU  CagU
26HPSH_04270828660..829418 [+], 759188527632cag pathogenicity island protein CagV  CagV
27HPSH_04275829423..831030 [+], 1608188527633cag pathogenicity island protein CagW  CagW
28HPSH_04280831082..832650 [+], 1569188527634cag pathogenicity island protein CagX  CagX
29HPSH_04285832665..838229 [+], 5565188527635cag pathogenicity island protein CagY  CagY
30HPSH_04290838219..838353 [+], 135188527636hypothetical protein 
31HPSH_04295838366..838965 [+], 600188527637cag pathogenicity island protein CagZ  CagZ
32HPSH_04300838970..839962 [+], 993188527638cag pathogenicity island protein cag alpha  Cagalpha
33HPSH_04305839971..842217 [+], 2247188527639cag pathogenicity island protein Cag beta  Cagbeta
34HPSH_04310842336..842716 [-], 381188527640cag pathogenicity island protein Cag gamma  Caggamma
35HPSH_04315842855..844300 [-], 1446188527641cag pathogenicity island protein Cag delta  Cagdelta
36HPSH_04320844314..844511 [-], 198188527642cag pathogenicity island protein Cag theta 
37HPSH_04325844527..844874 [-], 348188527643cag pathogenicity island protein Cag zeta 
38HPSH_04330845087..845917 [+], 831188527644hypothetical protein 
39HPSH_04335845992..846984 [-], 993188527645hypothetical protein 
40HPSH_04340 (era)846981..847886 [-], 906188527646GTP-binding protein Era 
41HPSH_04345 (hslU)847886..849217 [-], 1332188527647ATP-dependent protease ATP-binding subunit HslU 
 
accesspry protein This T4SS contains information of accessory protein.
interaction This T4SS contains information of interaction.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes

Effectors
CagA; Peptidoglycan

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1HPSH_04145 804109..807594 [-], 3486188527607cag pathogenicity island protein CagAexperimental  CagA
experimental T4SE derived from experimental literature.
interaction This effector contains information of interaction.

Download FASTA format files
Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1CagF (chaperone) (188527612)CT20aa and an intact N terminusCagAThe CagA-binding protein CagF is a secretion chaperone-like protein that interacts with a 100 aa region that is adjacent to the C-terminal secretion signal of CagA.CagF is a translocation factor for CagA, but is not translocated by the type IV apparatus.(1) PubMed: 17768234
Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Pattis I; Weiss E; Laugks R; Haas R; Fischer W (2007). The Helicobacter pylori CagF protein is a type IV secretion chaperone-like molecule that binds close to the C-terminal secretion signal of the CagA effector protein. Microbiology. 153(Pt 9):2896-909. [PudMed:17768234] experimental
The information on host-pathogen interaction.

#Name(Protein GI)Host site/SubstrateSourceFunctionReference
1CagL (188527617)integrin αvβ5humanCagL/integrin β5 signalling complex is important for gastrin expression induced by H. pylori.(1) PubMed: 22287591insolico
2CagL (188527617)integrin αvβ6humanαvβ6 is a specific, high affinity receptor for CagL.(2) PubMed: 31197920insolico

insolico This T4SE is highly identical with the protein mentioned in the literature.
(1) Wiedemann T et al. (2012). Helicobacter pylori CagL dependent induction of gastrin expression via a novel αvβ5-integrin-integrin linked kinase signalling complex. Gut. 61(7):986-96. [PudMed:22287591] experimental
(2) Buß M et al. (2019). Specific high affinity interaction of Helicobacter pylori CagL with integrin αV β6 promotes type IV secretion of CagA into human cells. FEBS J. . [PudMed:31197920] experimental
(1) You Y; He L; Zhang M; Fu J; Gu Y; Zhang B; Tao X; Zhang J (2012). Comparative Genomics of Helicobacter pylori Strains of China Associated with Different Clinical Outcome. PLoS One. 7(6):e38528. [PudMed:22701658] experimental in_silico
(2) Fischer W; Windhager L; Rohrer S; Zeiller M; Karnholz A; Hoffmann R; Zimmer R; Haas R (2010). Strain-specific genes of Helicobacter pylori: genome evolution driven by a novel type IV secretion system and genomic island transfer. Nucleic Acids Res. 38(18):6089-101. [PudMed:20478826] experimental in_silico
 
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation

Genomics & Bioinformatics Group in MML, SJTU