SecReT4
T4SS ID34
StrainHelicobacter pylori B8
Repliconchromosome [Browse all T4SS(s) in this replicon]
AccessionNC_014256
Location671072..701978
NameCag
Functioneffector translocation
ClassificationType IVA; Type P
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components
ComponentCagCCagDCagECagFCagGCagHCagICagLCagN
Number111111111
ComponentCagMCagTCagUCagVCagWCagXCagYCagZCagalpha
Number111111111
ComponentCagbetaCaggammaCagdelta
Number111

The information of T4SS components from NC_014256
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1HPB8_691 (nudT14)666318..666956 [+], 639298736186UDP-sugar diphosphatase 
2HPB8_692666967..668022 [+], 1056298736187plasminogen-binding protein PgbA 
3HPB8_693 (glcD1)668024..669403 [+], 1380298736188FAD linked oxidase-like protein 
4HPB8_694 (dapB)669425..670189 [+], 765298736189Dihydrodipicolinate reductase 
5HPB8_695670413..670523 [-], 111298736190hypothetical protein 
6HPB8_696 (cagB)670637..670864 [+], 228298736191cag pathogenicity island protein B 
7HPB8_697 (cagC)671072..671356 [+], 285298736192cag pathogenicity island protein C  CagC
8HPB8_698 (cagD)671340..671984 [+], 645298736193cag pathogenicity island protein D  CagD
9HPB8_699 (cagE)671999..674944 [+], 2946298736194cag pathogenicity island protein E  CagE
10HPB8_700 (cagF)674946..675752 [+], 807298736195cag pathogenicity island protein F  CagFaccesspry protein
11HPB8_701 (cagG)675807..676235 [+], 429298736196cag pathogenicity island protein G  CagG
12HPB8_702 (cagH)676237..677349 [+], 1113298736197cag pathogenicity island protein H  CagH
13HPB8_703 (cagI)677360..678505 [+], 1146298736198cag pathogenicity island protein I  CagI
14HPB8_704 (cagL)678502..679215 [+], 714298736199cag pathogenicity island protein L  CagLinteraction
15HPB8_705 (cagN)679297..680223 [-], 927298736200cag pathogenicity island protein N  CagN
16HPB8_706 (cagM)680232..681362 [-], 1131298736201cag pathogenicity island protein M  CagM
17HPB8_707681666..681782 [-], 117298736202hypothetical protein 
18HPB8_708682552..682854 [-], 303298736203hypothetical protein 
19HPB8_709 (cagQ)682562..682942 [+], 381298736204cag pathogenicity island protein Q 
20HPB8_710 (cagS)683384..683983 [+], 600298736205cag pathogenicity island protein S 
21HPB8_711 (cagT)684180..685022 [-], 843298736206cag pathogenicity island protein T  CagT
22HPB8_712 (cagU)685035..685685 [-], 651298736207cag pathogenicity island protein U  CagU
23HPB8_713 (cagV)686072..686830 [+], 759298736208cag pathogenicity island protein V  CagV
24HPB8_714 (cagW)686835..688433 [+], 1599298736209cag pathogenicity island protein W  CagW
25HPB8_715 (cagX)688486..690054 [+], 1569298736210cag pathogenicity island protein X  CagX
26HPB8_716 (cagY)690069..695534 [+], 5466298736211cag pathogenicity island protein Y  CagY
27HPB8_717 (cagZ)695683..696282 [+], 600298736212cag pathogenicity island protein Z  CagZ
28HPB8_718 (virB11-4)696287..697279 [+], 993298736213cag pathogenicity island protein alpha  Cagalpha
29HPB8_719 (cag5)697288..699534 [+], 2247298736214cag pathogenicity island protein beta  Cagbeta
30HPB8_720699579..699698 [-], 120298736215hypothetical protein 
31HPB8_721699618..699893 [+], 276298736216hypothetical protein 
32HPB8_722 (cag4)700014..700523 [-], 510298736217cag pathogenicity island protein gamma  Caggamma
33HPB8_723 (cag3)700533..701978 [-], 1446298736218cag pathogenicity island protein delta  Cagdelta
34HPB8_724 (cag2)701971..702675 [-], 705298736219cag pathogenicity island protein epsilon 
35HPB8_725 (cag1)702739..703086 [-], 348298736220cag pathogenicity island protein zeta 
36HPB8_726703259..704110 [+], 852298736221chitin synthase regulatory factor 3 
37HPB8_727704147..704326 [+], 180298736222hypothetical protein 
38HPB8_728 (glnA)704340..705785 [-], 1446298736223glutamine synthetase 
 
accesspry protein This T4SS contains information of accessory protein.
interaction This T4SS contains information of interaction.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes

Effectors
CagA; Peptidoglycan

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1HPB8_741 (cagA)716816..720370 [+], 3555298736236cytotoxin-associated protein A  CagA

interaction This effector contains information of interaction.

Download FASTA format files
Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1CagF (chaperone) (298736195)CT20aa and an intact N terminusCagAThe CagA-binding protein CagF is a secretion chaperone-like protein that interacts with a region that is adjacent to the C-terminal secretion signal of CagA.(1) PubMed: 17768234
Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Pattis I; Weiss E; Laugks R; Haas R; Fischer W (2007). The Helicobacter pylori CagF protein is a type IV secretion chaperone-like molecule that binds close to the C-terminal secretion signal of the CagA effector protein. Microbiology. 153(Pt 9):2896-909. [PudMed:17768234] experimental
The information on host-pathogen interaction.

#Name(Protein GI)Host site/SubstrateSourceFunctionReference
1CagL (298736199)integrin αvβ5humanCagL/integrin β5 signalling complex is important for gastrin expression induced by H. pylori.(1) PubMed: 22287591experimental
2CagL (298736199)integrin αvβ6humanαvβ6 is a specific, high affinity receptor for CagL.(2) PubMed: 31197920insolico
experimental This T4SE is mentioned in the literature.
insolico This T4SE is highly identical with the protein mentioned in the literature.
(1) Wiedemann T et al. (2012). Helicobacter pylori CagL dependent induction of gastrin expression via a novel αvβ5-integrin-integrin linked kinase signalling complex. Gut. 61(7):986-96. [PudMed:22287591] experimental
(2) Buß M et al. (2019). Specific high affinity interaction of Helicobacter pylori CagL with integrin αV β6 promotes type IV secretion of CagA into human cells. FEBS J. . [PudMed:31197920] experimental
(1) Farnbacher M; Jahns T; Willrodt D; Daniel R; Haas R; Goesmann A; Kurtz S; Rieder G (2010). Sequencing, annotation, and comparative genome analysis of the gerbil-adapted Helicobacter pylori strain B8. BMC Genomics. 11:335. [PudMed:20507619] in_silico
 
in_silico This literature contains bioinformatics investigation

Genomics & Bioinformatics Group in MML, SJTU