StrainBartonella henselae str. Houston-1
Repliconchromosome [Browse all T4SS(s) in this replicon]
Functioneffector translocation
ClassificationType IVA; Type P
experimental Experimental investigation has been performed on this T4SS.
structure Information of structure: structureBepA, BepE

T4SS components

The information of T4SS components from NC_005956
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1BH132501511669..1512412 [-], 74449476009hypothetical protein 
2BH13260 (virB2)1512780..1513103 [+], 32449476010virB2 protein  VirB2
3BH13270 (virB3)1513104..1513415 [+], 31249476011virB3 protein  VirB3
4BH13280 (virB4)1513421..1515775 [+], 235549476012virB4 protein  VirB4
5BH132901515772..1516218 [+], 44749476013hypothetical protein  VirB5
6BH13300 (virB6)1516205..1517170 [+], 96649476014virB protein  VirB6
7BH133101517298..1517708 [+], 41149476015hypothetical protein  VirB7
8BH13320 (virB8)1517698..1518366 [+], 66949476016virB8 protein  VirB8
9BH13330 (virB9)1518366..1519229 [+], 86449476017virB9 protein  VirB9
10BH13340 (virB10)1519222..1520412 [+], 119149476018virB10 protein  VirB10
11BH13350 (virB11)1520381..1521451 [+], 107149476019virB11 protein  VirB11
12BH133601521519..1521725 [+], 20749476020hypothetical protein 
13BH133701521736..1523370 [+], 163549476021cell filamentation protein  interaction
14BH13380 (traG)1523533..1525452 [+], 192049476022conjugal transfer protein TraG  VirD4
15BH133901525994..1527622 [+], 162949476023hypothetical protein 
16BH134001528692..1530290 [+], 159949476024cell filamentation protein  interaction
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

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Proteins        Genes
Crucial for establishment of infection in the primary niche- the endothelial cells, required for colonization of endothelial cells, by mediating the intracellular delivery of effector proteins (BepA-BepG). Translocation of the Beps provokes distinct cellular phenotypes: (i) a massive cytoskeleton rearrangement, resulting in the engulfment of bacterial aggregates (a phenotype called 'invasome' formation), (ii) NF-kappaB-dependent proinflammatory activation, characterized by chemokine secretion and expression of cell adhesion proteins, and (iii) enhanced cell survival as a result of anti-apoptosis (iv) capillary-like sprout formation of endothelial cell aggregates

BepA, BepB, BepC, BepD, BepE, BepF, BepG

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1BH13370 1521736..1523370 [+], 163549476021cell filamentation proteinexperimental  BepAinteraction
2BH13390 1525994..1527622 [+], 162949476023hypothetical proteinexperimental  BepB
3BH13400 1528692..1530290 [+], 159949476024cell filamentation proteinexperimental  BepCinteraction
4BH13410 1530764..1532368 [+], 160549476025hypothetical proteinexperimental  BepD
5BH13420 1532694..1534088 [+], 139549476026hypothetical proteinexperimental  BepEinteraction
6BH13430 1534424..1536928 [+], 250549476027hypothetical proteinexperimental  BepFinteraction
7BH13440 1537113..1540244 [+], 313249476028hypothetical proteinexperimental  BepGinteraction
experimental T4SE derived from experimental literature.
interaction This effector contains information of interaction.

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Proteins        Genes
The information on structure of this T4ss

1BepA2JK8 PDB (2JK8)Type IV secretion system effector protein BepA complexed with a pyrophosphate moiety.(1) PubMed: 21213248
2BepE4YK3 PDB (4YK3)Crystal Structure of the BID Domain of BepE from Bartonella henselae.(2) PubMed: 27889208
(1) Palanivelu DV; Goepfert A; Meury M; Guye P; Dehio C; Schirmer T (2011). Fic domain-catalyzed adenylylation: insight provided by the structural analysis of the type IV secretion system effector BepA. Protein Sci. 20(3):492-9. [PudMed:21213248] experimental
(2) Stanger FV et al. (2017). The BID Domain of Type IV Secretion Substrates Forms a Conserved Four-Helix Bundle Topped with a Hook. Structure. 25(1):203-211. [PudMed:27889208] experimental
The information on host-pathogen interaction.

#Name(Protein GI)Host site/SubstrateSourceFunctionReference
1BepA (49476021)Adenylyl cyclasehumanBepA is located on the plasma membrane and triggers the production of second messenger cyclic adenosine monophosphate to repress endothelial cell (EC) apoptosis.(1) PubMed: 17121462experimental
2BepC (49476024)unknownunknownThe combined action of BepC and BepF triggers the formation of invasome and repress endocytic uptake of inert microspheres.(2) PubMed: 20964799experimental
3BepE (49476026)unknownunknownBepE protects infected migratory cells from BepC's injurious and restores normal cell migration through the RhoA signaling pathway.(3) PubMed: 24945914experimental
4BepF (49476027)unknownunknownThe combined action of BepC and BepF triggers the formation of invasome and repress endocytic uptake of inert microspheres. Besides, BepF play a role in activation of Rac1 and Cdc42 during the invasome formation process.(4) PubMed: 22043280experimental
5BepG (49476028)unknownunknownBepG repress bacterial endocytosis to triggers invasome-mediated internalization.(5) PubMed: 19302579experimental
experimental This T4SE is mentioned in the literature.

(1) Schmid MC; Scheidegger F; Dehio M; Balmelle-Devaux N; Schulein R; Guye P; Chennakesava CS; Biedermann B; Dehio C (2006). A translocated bacterial protein protects vascular endothelial cells from apoptosis. PLoS Pathog. 2(11):e115. [PudMed:17121462] experimental
(2) Truttmann MC; Rhomberg TA; Dehio C (2011). Combined action of the type IV secretion effector proteins BepC and BepF promotes invasome formation of Bartonella henselae on endothelial and epithelial cells. Cell Microbiol. 13(2):284-99. [PudMed:20964799] experimental
(3) Okujava R et al. (2014). A translocated effector required for Bartonella dissemination from derma to blood safeguards migratory host cells from damage by co-translocated effectors. PLoS Pathog. 10(6):e1004187. [PudMed:24945914] experimental
(4) Truttmann MC; Guye P; Dehio C (2011). BID-F1 and BID-F2 domains of Bartonella henselae effector protein BepF trigger together with BepC the formation of invasome structures. PLoS One. 6(10):e25106. [PudMed:22043280] experimental in_silico
(5) Rhomberg TA; Truttmann MC; Guye P; Ellner Y; Dehio C (2009). A translocated protein of Bartonella henselae interferes with endocytic uptake of individual bacteria and triggers uptake of large bacterial aggregates via the invasome. Cell Microbiol. 11(6):927-45. [PudMed:19302579] experimental
(1) Guy L; Nystedt B; Sun Y; Naslund K; Berglund EC; Andersson SG (2012). A genome-wide study of recombination rate variation in Bartonella henselae. BMC Evol Biol. 12(1):65. [PudMed:22577862] in_silico
(2) Truttmann MC; Misselwitz B; Huser S; Hardt WD; Critchley DR; Dehio C (2011). Bartonella henselae engages inside-out and outside-in signaling by integrin beta1 and talin1 during invasome-mediated bacterial uptake. J Cell Sci. 124(Pt 21):3591-602. [PudMed:22045736] experimental
(3) Fernandez-Gonzalez E; de Paz HD; Alperi A; Agundez L; Faustmann M; Sangari FJ; Dehio C; Llosa M (2011). Transfer of R388 derivatives by a pathogenesis-associated type IV secretion system into both bacteria and human cells. J Bacteriol. 193(22):6257-65. [PudMed:21908662] experimental
(4) Schroder G; Schuelein R; Quebatte M; Dehio C (2011). Conjugative DNA transfer into human cells by the VirB/VirD4 type IV secretion system of the bacterial pathogen Bartonella henselae. Proc Natl Acad Sci U S A. 108(35):14643-8. [PudMed:21844337] experimental
(5) Scheidegger F; Quebatte M; Mistl C; Dehio C (2011). The Bartonella henselae VirB/Bep system interferes with vascular endothelial growth factor (VEGF) signalling in human vascular endothelial cells. Cell Microbiol. 13(3):419-31. [PudMed:21044238] experimental
(6) Truttmann MC; Rhomberg TA; Dehio C (2011). Combined action of the type IV secretion effector proteins BepC and BepF promotes invasome formation of Bartonella henselae on endothelial and epithelial cells. Cell Microbiol. 13(2):284-99. [PudMed:20964799] experimental
(7) Quebatte M; Dehio M; Tropel D; Basler A; Toller I; Raddatz G; Engel P; Huser S; Schein H; Lindroos HL; Andersson SG; Dehio C (2010). The BatR/BatS two-component regulatory system controls the adaptive response of Bartonella henselae during human endothelial cell infection. J Bacteriol. 192(13):3352-67. [PudMed:20418395] experimental
(8) Scheidegger F; Ellner Y; Guye P; Rhomberg TA; Weber H; Augustin HG; Dehio C (2009). Distinct activities of Bartonella henselae type IV secretion effector proteins modulate capillary-like sprout formation. Cell Microbiol. 11(7):1088-101. [PudMed:19416269] experimental
(9) Schulein R; Guye P; Rhomberg TA; Schmid MC; Schroder G; Vergunst AC; Carena I; Dehio C (2005). A bipartite signal mediates the transfer of type IV secretion substrates of Bartonella henselae into human cells. Proc Natl Acad Sci U S A. 102(3):856-61. [PudMed:15642951] experimental
(10) Schmid MC; Schulein R; Dehio M; Denecker G; Carena I; Dehio C (2004). The VirB type IV secretion system of Bartonella henselae mediates invasion, proinflammatory activation and antiapoptotic protection of endothelial cells. Mol Microbiol. 52(1):81-92. [PudMed:15049812] experimental
(11) Schmiederer M; Arcenas R; Widen R; Valkov N; Anderson B (2001). Intracellular induction of the Bartonella henselae virB operon by human endothelial cells. Infect Immun. 69(10):6495-502. [PudMed:11553594] experimental
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation