SecReT4
T4SS ID1
StrainBrucella melitensis bv. 1 str. 16M
Repliconchromosome II [Browse all T4SS(s) in this replicon]
AccessionNC_003318
Location24862..35272
NameVirB
Functioneffector translocation
ClassificationType IVA; Type P
experimental Experimental investigation has been performed on this T4SS.

T4SS components
ComponentVirB1VirB2VirB3VirB4VirB5VirB6VirB7VirB8VirB9
Number111111111
ComponentVirB10VirB11VirD4
Number110

The information of T4SS components from NC_003318
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1BMEII002220563..21762 [+], 120017988366hypothetical protein 
2BMEII002321815..22708 [-], 89417988367UTP-glucose-1-phosphate uridylyltransferase 
3BMEII002423120..24358 [+], 123917988368membrane-bound lytic murein transglycosylase B 
4BMEII002524862..25578 [+], 71717988369attachment mediating protein VIRB1-like protein  VirB1
5BMEII002626014..26331 [+], 31817988370VirB2  VirB2
6BMEII002726345..26695 [+], 35117988371VirB3  VirB3
7BMEII002826695..29190 [+], 249617988372ATPase VIRB4-like protein  VirB4
8BMEII002929195..29911 [+], 71717988373VirB5  VirB5
9BMEII003030095..31138 [+], 104417988374channel protein VIRB6-like protein  VirB6
10BMEII003131302..31475 [+], 17417988375VirB7  VirB7
11BMEII003231478..32197 [+], 72017988376VirB8  VirB8
12BMEII003332321..33064 [+], 74417988377channel protein VIRB9-like protein  VirB9
13BMEII003433406..34203 [+], 79817988378channel protein VIRB10-like protein  VirB10
14BMEII003534187..35272 [+], 108617988379ATPase VIRB11-like protein  VirB11
15BMEII003635418..35936 [+], 51917988380outer membrane protein OprF 
16BMEII003736376..37347 [+], 97217988381cytoplasmic protein 
17BMEII003837467..38960 [+], 149417988382D-serine/D-alanine/glycine transporter 
 
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes

Effectors
BPE005, VceA, RicA, BPE275, VceC, BPE043, BtpB, BPE123

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1BMEI0067 65562..66023 [+], 46217986351cAMP-dependent protein kinase regulatory subunit  BPE005
2BMEI0390 406254..406517 [-], 26417986673hypothetical protein  VceA
3BMEI0736 762720..763247 [+], 52817987019ferripyochelin binding proteinexperimental  RicAinteraction
4BMEI0739 765229..765936 [+], 70817987022rhomboid family integral membrane protein  BPE275
5BMEI0948 984798..985883 [+], 108617987231hypothetical protein  VceC
6BMEI0961 995691..999671 [+], 398117987244kinesin-like protein  BPE043
7BMEI1216 1264446..1264982 [-], 53717987499hypothetical proteinexperimental  BtpBinteraction
8BMEII1111 1147329..1147790 [+], 46217989456hypothetical protein  BPE123

interaction This effector contains information of interaction.

Download FASTA format files
Proteins        Genes
The information on host-pathogen interaction.

#Name(Protein GI)Host site/SubstrateSourceFunctionReference
1BtpB (17987499)MYD88murineBtpB may interact with MYD88 to repress TLR signaling and interfere with activation of dendritic cells. (1) PubMed: 23847770insolico
2RicA (17987019)small GTPase Rab2humanRicA interacted with human small GTPase Rab2 involved in trafficking.(2) PubMed: 21501366experimental
experimental This T4SE is mentioned in the literature.
insolico This T4SE is highly identical with the protein mentioned in the literature.
(1) Salcedo SP et al. (2013). BtpB, a novel Brucella TIR-containing effector protein with immune modulatory functions. . Front. Cell. Infect. Microbiol. . 2013.00028 . [PudMed:23847770] experimental
(2) de Barsy M; Jamet A; Filopon D; Nicolas C; Laloux G; Rual JF; Muller A; Twizere JC; Nkengfac B; Vandenhaute J; Hill DE; Salcedo SP; Gorvel JP; Letesson JJ; De Bolle X (2011). Identification of a Brucella spp. secreted effector specifically interacting with human small GTPase Rab2. Cell Microbiol. 13(7):1044-58. [PudMed:21501366] experimental
(1) de Jong MF; Tsolis RM (2012). Brucellosis and type IV secretion. Future Microbiol. 7(1):47-58. [PudMed:22191446]
(2) Dozot M; Poncet S; Nicolas C; Copin R; Bouraoui H; Maze A; Deutscher J; De Bolle X; Letesson JJ (2010). Functional characterization of the incomplete phosphotransferase system (PTS) of the intracellular pathogen Brucella melitensis. PLoS One. 5(9). [PudMed:20844759] experimental
(3) Wang Y; Chen Z; Qiao F; Zhong Z; Xu J; Wang Z; Du X; Qu Q; Yuan J; Jia L; Song H; Sun Y; Huang L (2010). The type IV secretion system affects the expression of Omp25/Omp31 and the outer membrane properties of Brucella melitensis. FEMS Microbiol Lett. 303(1):92-100. [PudMed:20030728] experimental
(4) Paixao TA; Roux CM; den Hartigh AB; Sankaran-Walters S; Dandekar S; Santos RL; Tsolis RM (2009). Establishment of systemic Brucella melitensis infection through the digestive tract requires urease, the type IV secretion system, and lipopolysaccharide O antigen. Infect Immun. 77(10):4197-208. [PudMed:19651862] experimental
(5) Zhong Z; Wang Y; Qiao F; Wang Z; Du X; Xu J; Zhao J; Qu Q; Dong S; Sun Y; Huang L; Huang K; Chen Z (2009). Cytotoxicity of Brucella smooth strains for macrophages is mediated by increased secretion of the type IV secretion system. Microbiology. 155(Pt 10):3392-402. [PudMed:19628564] experimental
(6) Wang Y; Chen Z; Qiao F; Ying T; Yuan J; Zhong Z; Zhou L; Du X; Wang Z; Zhao J; Dong S; Jia L; Yuan X; Yang R; Sun Y; Huang L (2009). Comparative proteomics analyses reveal the virB of B. melitensis affects expression of intracellular survival related proteins. PLoS One. 4(4):e5368. [PudMed:19401764] experimental
(7) Nijskens C; Copin R; De Bolle X; Letesson JJ (2008). Intracellular rescuing of a B. melitensis 16M virB mutant by co-infection with a wild type strain. Microb Pathog. 45(2):134-41. [PudMed:18547782] experimental
(8) Rambow-Larsen AA; Rajashekara G; Petersen E; Splitter G (2008). Putative quorum-sensing regulator BlxR of Brucella melitensis regulates virulence factors including the type IV secretion system and flagella. J Bacteriol. 190(9):3274-82. [PudMed:18310341] experimental
(9) Rolan HG; den Hartigh AB; Kahl-McDonagh M; Ficht T; Adams LG; Tsolis RM (2008). VirB12 is a serological marker of Brucella infection in experimental and natural hosts. Clin Vaccine Immunol. 15(2):208-14. [PudMed:18077620] experimental
(10) Pei J; Wu Q; Kahl-McDonagh M; Ficht TA (2008). Cytotoxicity in macrophages infected with rough Brucella mutants is type IV secretion system dependent. Infect Immun. 76(1):30-7. [PudMed:17938217] experimental
(11) Uzureau S; Godefroid M; Deschamps C; Lemaire J; De Bolle X; Letesson JJ (2007). Mutations of the quorum sensing-dependent regulator VjbR lead to drastic surface modifications in Brucella melitensis. J Bacteriol. 189(16):6035-47. [PudMed:17557825] experimental
(12) Roux CM; Rolan HG; Santos RL; Beremand PD; Thomas TL; Adams LG; Tsolis RM (2007). Brucella requires a functional Type IV secretion system to elicit innate immune responses in mice. Cell Microbiol. 9(7):1851-69. [PudMed:17441987] experimental
(13) Delory M; Hallez R; Letesson JJ; De Bolle X (2006). An RpoH-like heat shock sigma factor is involved in stress response and virulence in Brucella melitensis 16M. J Bacteriol. 188(21):7707-10. [PudMed:16936018] experimental
(14) Dozot M; Boigegrain RA; Delrue RM; Hallez R; Ouahrani-Bettache S; Danese I; Letesson JJ; De Bolle X; Kohler S (2006). The stringent response mediator Rsh is required for Brucella melitensis and Brucella suis virulence, and for expression of the type IV secretion system virB. Cell Microbiol. 8(11):1791-802. [PudMed:16803581] experimental
(15) Sun YH; Rolan HG; den Hartigh AB; Sondervan D; Tsolis RM (2005). Brucella abortus virB12 is expressed during infection but is not an essential component of the type IV secretion system. Infect Immun. 73(9):6048-54. [PudMed:16113325] experimental
(16) Delrue RM; Deschamps C; Leonard S; Nijskens C; Danese I; Schaus JM; Bonnot S; Ferooz J; Tibor A; De Bolle X; Letesson JJ (2005). A quorum-sensing regulator controls expression of both the type IV secretion system and the flagellar apparatus of Brucella melitensis. Cell Microbiol. 7(8):1151-61. [PudMed:16008582] experimental
(17) Rouot B; Alvarez-Martinez MT; Marius C; Menanteau P; Guilloteau L; Boigegrain RA; Zumbihl R; O'Callaghan D; Domke N; Baron C (2003). Production of the type IV secretion system differs among Brucella species as revealed with VirB5- and VirB8-specific antisera. Infect Immun. 71(3):1075-82. [PudMed:12595417] experimental
 
experimental This literature contains experimental investigation

Genomics & Bioinformatics Group in MML, SJTU