Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ABFJ87_RS08540 Genome accession   NZ_CP155816
Coordinates   1717243..1717740 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain CUBIST-18     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1712243..1722740
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFJ87_RS08515 (ABFJ87_08540) gcvPB 1712405..1713877 (-) 1473 WP_406848729.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  ABFJ87_RS08520 (ABFJ87_08545) gcvPA 1713870..1715216 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ABFJ87_RS08525 (ABFJ87_08550) gcvT 1715236..1716327 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  ABFJ87_RS08530 (ABFJ87_08555) - 1716486..1717010 (-) 525 WP_001015120.1 shikimate kinase -
  ABFJ87_RS08535 (ABFJ87_08560) - 1717000..1717146 (-) 147 WP_001792109.1 hypothetical protein -
  ABFJ87_RS08540 (ABFJ87_08565) comGF 1717243..1717740 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABFJ87_RS08545 (ABFJ87_08570) comGE 1717658..1717957 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  ABFJ87_RS08550 (ABFJ87_08575) comGD 1717944..1718390 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABFJ87_RS08555 (ABFJ87_08580) comGC 1718368..1718679 (-) 312 WP_031786220.1 competence type IV pilus major pilin ComGC Machinery gene
  ABFJ87_RS08560 (ABFJ87_08585) comGB 1718693..1719763 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABFJ87_RS08565 (ABFJ87_08590) comGA 1719735..1720709 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ABFJ87_RS08570 (ABFJ87_08595) - 1720761..1721384 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ABFJ87_RS08575 (ABFJ87_08600) - 1721381..1721710 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ABFJ87_RS08580 (ABFJ87_08605) - 1721710..1722696 (-) 987 WP_000161311.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=999499 ABFJ87_RS08540 WP_001788897.1 1717243..1717740(-) (comGF) [Staphylococcus aureus strain CUBIST-18]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=999499 ABFJ87_RS08540 WP_001788897.1 1717243..1717740(-) (comGF) [Staphylococcus aureus strain CUBIST-18]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment