Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SriyCCGPB3_RS02440 Genome accession   NZ_CP155568
Coordinates   526036..527163 (-) Length   375 a.a.
NCBI ID   WP_032127971.1    Uniprot ID   A0AA43AM75
Organism   Stenotrophomonas riyadhensis strain ESTM1A_MKCTX4_10a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 521036..532163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SriyCCGPB3_RS02425 (SriyCCGPB3_000484) - 521894..523309 (-) 1416 WP_049461652.1 DEAD/DEAH box helicase -
  SriyCCGPB3_RS02430 (SriyCCGPB3_000485) - 524286..525275 (+) 990 WP_153856227.1 fumarylacetoacetate hydrolase family protein -
  SriyCCGPB3_RS02435 (SriyCCGPB3_000486) maiA 525290..525958 (+) 669 WP_110710961.1 maleylacetoacetate isomerase -
  SriyCCGPB3_RS02440 (SriyCCGPB3_000487) pilU 526036..527163 (-) 1128 WP_032127971.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SriyCCGPB3_RS02445 (SriyCCGPB3_000488) - 527285..527650 (+) 366 WP_005415160.1 DUF4398 domain-containing protein -
  SriyCCGPB3_RS02450 (SriyCCGPB3_000489) - 527647..528531 (+) 885 WP_164162109.1 hypothetical protein -
  SriyCCGPB3_RS02455 (SriyCCGPB3_000490) - 528856..529092 (+) 237 WP_005407964.1 YdcH family protein -
  SriyCCGPB3_RS02460 (SriyCCGPB3_000491) - 529393..530763 (+) 1371 WP_153856229.1 pyridoxal-phosphate dependent enzyme -
  SriyCCGPB3_RS02465 (SriyCCGPB3_000492) - 530887..532071 (+) 1185 WP_153856230.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41453.78 Da        Isoelectric Point: 6.3741

>NTDB_id=997805 SriyCCGPB3_RS02440 WP_032127971.1 526036..527163(-) (pilU) [Stenotrophomonas riyadhensis strain ESTM1A_MKCTX4_10a]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEGSLEEGMESFDQCL
FRLAKAGIIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGQPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=997805 SriyCCGPB3_RS02440 WP_032127971.1 526036..527163(-) (pilU) [Stenotrophomonas riyadhensis strain ESTM1A_MKCTX4_10a]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTCTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTCTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGCCCGATGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTCATCCGCGCCATCCGCAGCCGCATCCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGTGGGCTGGTGCTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGACACCCATGCCTTCCATAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCGGAGACGATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAGGGCCGTCGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCCGCGATGGAAGGCTCGCTGGAGGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCGGGCATCATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAGCACGACCCATACGCCGATTTCTCGGCCGGGCAGCCAAGCATCACTCACG
GGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.81

95.467

0.437
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.765

90.667

0.379
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363

Multiple sequence alignment