Detailed information NTG0997736

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: KZZ19_RS05690
• Genome accession: NZ_CP155559
• Coordinates: 1107888..1111613 (+)
• Length: 1241 a.a.
• NCBI ID: WP_237979809.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis strain HSY204
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1102888..1116613

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KZZ19_RS05675 (KZZ19_005675)	-	1103046..1103639 (+)	594	WP_043936619.1	TVP38/TMEM64 family protein	-
KZZ19_RS05680 (KZZ19_005680)	lepB	1103696..1104259 (+)	564	WP_088095496.1	signal peptidase I	-
KZZ19_RS05685 (KZZ19_005685)	addB	1104376..1107891 (+)	3516	WP_237979807.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
KZZ19_RS05690 (KZZ19_005690)	addA	1107888..1111613 (+)	3726	WP_237979809.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
KZZ19_RS05695 (KZZ19_005695)	-	1111626..1111913 (+)	288	WP_237979811.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
KZZ19_RS05700 (KZZ19_005700)	gerPF	1111995..1112210 (-)	216	WP_001141570.1	spore germination protein GerPF	-
KZZ19_RS05705 (KZZ19_005705)	-	1112253..1112639 (-)	387	WP_237979813.1	spore germination protein GerPE	-
KZZ19_RS05710 (KZZ19_005710)	gerPD	1112655..1112849 (-)	195	WP_001052807.1	spore germination protein GerPD	-
KZZ19_RS05715 (KZZ19_005715)	gerPC	1112856..1113470 (-)	615	WP_088095505.1	spore germination protein GerPC	-
KZZ19_RS05720 (KZZ19_005720)	gerPB	1113538..1113744 (-)	207	WP_001012506.1	spore germination protein GerPB	-
KZZ19_RS05725 (KZZ19_005725)	gerPA	1113759..1113980 (-)	222	WP_001111187.1	spore germination protein GerPA	-
KZZ19_RS05730 (KZZ19_005730)	-	1114075..1114254 (-)	180	WP_088095506.1	aspartyl-phosphate phosphatase Spo0E family protein	-
KZZ19_RS05735 (KZZ19_005735)	-	1114501..1115400 (+)	900	WP_170930122.1	fumarylacetoacetate hydrolase family protein	-
KZZ19_RS05740 (KZZ19_005740)	-	1115435..1115719 (-)	285	WP_098343164.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142760.08 Da
• Isoelectric Point: 4.8994

>NTDB_id=997736 KZZ19_RS05690 WP_237979809.1 1107888..1111613(+) (addA) [Bacillus thuringiensis strain HSY204]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEEDPVDVDRLLVVTFTNAAAQEMKNRV
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEAEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVKAYDVEGKTIEDLVYASYLLEDVKFQLETAEAHI
RKATELAMLPDGPAPRVETLQADATLLRTLSSAACESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSESGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLAGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPIGEDVAAELLCIQQTEEEVQDGEEGTEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKNDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRTMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSDWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPGEIYDYDMSWK
VEVVDGKTLLAPEPVQEEKQELLEALREKKAVPLQSERKDEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSDETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKTIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=997736 KZZ19_RS05690 WP_237979809.1 1107888..1111613(+) (addA) [Bacillus thuringiensis strain HSY204]
ATGATTGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAACGGACG
TGATATTTTAGTTGCAGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAGATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCGGCGCAAGAGATGAAAAACAGAGTT
GGGGAAGCGTTAGAAAAAGTATTGATTGATGAGCCAGGATCGCAGCACATCAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCTATTTCGACAATCCATTCATTTTGTTTACAAGTCATTAGAGGATACTACTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACGGAAAATGAATTGTTAAAAGAAGAAGTATTAGATGACATATTAGAAGCAGAGTATGGAATTGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGATCGTAGTGATGATGACTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAAAAGCATACGACGTAGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTATCTATTAGAAGATGTGAAATTTCAGCTTGAAACAGCAGAGGCGCATATT
CGTAAAGCGACTGAACTCGCAATGCTTCCTGACGGTCCGGCGCCCCGTGTTGAAACCTTGCAAGCAGATGCAACTTTACT
TAGAACGTTATCGTCAGCTGCTTGTGAGTCATGGACAAGTGTGTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAAAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCTGTATTGGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGGGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAGTGGTGAAATGAAGCCATCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAGGATACGAACTTTGTGCAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGGAACTTGTTCATGGTTGGCGACGTAAAACAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGCTTATTCCTAGGAAAGTATAAACGCTTTACGCAAGAAGGATTAGCCGGCGGAATGAAGATTGACTTAGCGAAA
AATTTCCGTAGTCGTCATGAAGTGTTAGCAGGAACGAACTTCATCTTCAAACAAATTATGGGTGAAGAAGTTGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAATAGGTGAAGATGTAGCAGCTGAACTATTATGCA
TTCAGCAAACTGAAGAAGAAGTGCAAGACGGAGAAGAAGGTACCGAAGTAGAAAAAGCGCAGCTTGAAGCTCGTCTTATG
GCGCAGCGCATCAAAGCGATGGTCGATTCAGGTTATGAAGTGTATGACCGTAAAAATGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGTTCGATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCAACAGAAGTAAATATTATGATGAATGTATTTCGCGTGATTGATAAT
CCAATGCAAGATATTCCGCTTGCCGCCGTACTTCGTTCGCCAATCGTTGGACTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGTTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAAGAACAAGAAT
TACATGATAAATTAGAGTGGTTCTATAACTTACTGCAAGGATGGCGTGAATTTGCGCGCCAACAGTCTCTTTCTGATTTG
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTTGTCGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGCGCAAGGCAATATGAAGCAACATCGTTTAGAGGGTTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGTACTATGACGATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTGTTTGTTGCGGGACTAGGTCGCCGTTTTAATACACAAGACTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAGTTTATCGACCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTATTATACGTAGCGTTAACGCGT
GCAAAAGAAAAGCTAATTTTAATCGGAACAGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTCGATGCAAGGGAGCA
TAGTGACTGGTTATTACCTGATCACATTCGTGCCGGAGCGTCTTGTTATTTAGATTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCAGGTGAAATTTATGACTATGATATGAGCTGGAAA
GTAGAAGTTGTTGACGGCAAAACGTTACTCGCACCAGAACCGGTTCAAGAAGAGAAACAAGAATTACTAGAAGCACTTCG
TGAGAAAAAAGCGGTTCCGCTACAAAGTGAACGAAAAGATGAAGTATACGACAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCACCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGCAGCGATAATGCC
TTTATTAAAAAACTACGTGCGCCAATTAGAACACGTCCTCGTTTCATGGAGAAAAAAGGATTAACGTACGCTGAAAGAGG
TACGGCAGTTCACGCTGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGAAGAAGTGATTCGAGAGCAAATTGCCG
GAATGGTAAATAAGGAATTATTAACATTCGAACAAGCTGAAGAAATAGCAATTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAGAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAGGC
GTATCAAGATTGGCAAGGTAAGAGTGACGAAACGATACTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATCACTTTAATCGATTTTAAAACGGATACGATTGAAGGGAAGTTCCCAGGTGGATTTGATCAAGCGAAAACGATTTTA
GAAGATCGATATAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAGAAAAGCTTGCAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGCAATCATGTTGTAAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1059-1232)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.263	100	0.533