Detailed information NTG0997524

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: EL041_RS01060
• Genome accession: NZ_LR134092
• Coordinates: 222896..223879 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain NCTC10444
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 217896..228879

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL041_RS01040 (NCTC10444_00201)	dppA	219039..220646 (+)	1608	WP_001222876.1	dipeptide ABC transporter substrate-binding protein DppA	-
EL041_RS25490	-	220709..220936 (-)	228	WP_032153267.1	hypothetical protein	-
EL041_RS01050 (NCTC10444_00202)	dppB	220954..221973 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
EL041_RS01055 (NCTC10444_00203)	dppC	221983..222885 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
EL041_RS01060 (NCTC10444_00204)	amiE	222896..223879 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
EL041_RS01065 (NCTC10444_00205)	dppF	223876..224880 (+)	1005	WP_000107036.1	dipeptide ABC transporter ATP-binding subunit DppF	-
EL041_RS01070 (NCTC10444_00206)	yhjV	224910..226181 (-)	1272	WP_001313380.1	aromatic amino acid transport family protein	-
EL041_RS01075 (NCTC10444_00208)	-	226657..226764 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
EL041_RS01080 (NCTC10444_00210)	-	227140..227247 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
EL041_RS25655 (NCTC10444_00212)	-	227623..227730 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=997524 EL041_RS01060 WP_001196486.1 222896..223879(+) (amiE) [Escherichia coli strain NCTC10444]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=997524 EL041_RS01060 WP_001196486.1 222896..223879(+) (amiE) [Escherichia coli strain NCTC10444]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTGAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCTGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAATCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGCCGTCAGCGGGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTAGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404