Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAGS40_RS01735 Genome accession   NZ_CP155073
Coordinates   380256..381770 (-) Length   504 a.a.
NCBI ID   WP_345812796.1    Uniprot ID   -
Organism   Paraburkholderia sp. PREW-6R     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 375256..386770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGS40_RS01710 (AAGS40_01710) - 376091..376726 (-) 636 WP_345812792.1 LON peptidase substrate-binding domain-containing protein -
  AAGS40_RS01715 (AAGS40_01715) rapZ 376766..377659 (-) 894 WP_345812793.1 RNase adapter RapZ -
  AAGS40_RS01720 (AAGS40_01720) hprK 377850..378818 (-) 969 WP_007179412.1 HPr(Ser) kinase/phosphatase -
  AAGS40_RS01725 (AAGS40_01725) - 378939..379454 (-) 516 WP_345812794.1 PTS sugar transporter subunit IIA -
  AAGS40_RS01730 (AAGS40_01730) raiA 379728..380084 (-) 357 WP_345812795.1 ribosome-associated translation inhibitor RaiA -
  AAGS40_RS01735 (AAGS40_01735) rpoN/rpoN1 380256..381770 (-) 1515 WP_345812796.1 RNA polymerase factor sigma-54 Machinery gene
  AAGS40_RS01740 (AAGS40_01740) lptB 382012..382797 (-) 786 WP_345812797.1 LPS export ABC transporter ATP-binding protein -
  AAGS40_RS01745 (AAGS40_01745) lptA 382794..383465 (-) 672 WP_345812798.1 lipopolysaccharide transport periplasmic protein LptA -
  AAGS40_RS01750 (AAGS40_01750) lptC 383496..384095 (-) 600 WP_345812799.1 LPS export ABC transporter periplasmic protein LptC -
  AAGS40_RS01755 (AAGS40_01755) - 384092..384652 (-) 561 WP_345812800.1 HAD family hydrolase -
  AAGS40_RS01760 (AAGS40_01760) kdsD 384652..385635 (-) 984 WP_345812801.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 504 a.a.        Molecular weight: 55210.19 Da        Isoelectric Point: 5.1518

>NTDB_id=996117 AAGS40_RS01735 WP_345812796.1 380256..381770(-) (rpoN/rpoN1) [Paraburkholderia sp. PREW-6R]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVSMAISQNPLLENEDDWIASPLRVAADGSLIAQAPGSSAPPDQM
GGNVSSSSSSGDRAENGEPQGVDEYNGLSGDGNGDASQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVTS
AGQRDRALITFLIESLDDDGYLSATLEEVQADLPEELEVDQDELNAALALLHSFDPAGVGARSASECLRLQLLRLPGSPT
RTLALEIVAHHLELLAARDFTRLRKHLKASDDDLRDAHVLIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNPE
VVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIAD
TLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENSKSPLSDSRIAELLAEQGFV
VARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1515 bp        

>NTDB_id=996117 AAGS40_RS01735 WP_345812796.1 380256..381770(-) (rpoN/rpoN1) [Paraburkholderia sp. PREW-6R]
ATGAAAGCCAGTCTCCAACTCCGCCTATCGCAGCATCTTGCGCTGACCCCGCAGCTGCAGCAGTCCATCCGGCTGCTGCA
GTTGTCTACGCTCGAACTGCAACAGGAAGTCTCCATGGCGATCTCGCAGAATCCGCTCCTCGAGAACGAGGACGACTGGA
TCGCGAGCCCGCTTCGCGTGGCGGCGGACGGCTCGCTGATCGCTCAGGCGCCTGGCTCATCGGCGCCGCCCGACCAGATG
GGCGGCAACGTTTCGTCATCGTCCAGCAGCGGCGATCGCGCCGAAAACGGCGAACCCCAAGGCGTGGATGAATACAACGG
CCTCTCGGGCGACGGTAACGGCGATGCTTCGCAGTGGAATCTGGATGACTACGGCCGCTCCGGCAACGCTTCCGACGACG
ACGATCTGCCGCCTCTGCAGATCCACGAATCGAGCACTTCGCTGCGCGATCATCTGATGGCGCAATTGCGCGTGACTTCG
GCGGGTCAACGCGACCGCGCGCTGATCACCTTCCTGATCGAATCGCTCGACGACGATGGCTATCTTTCCGCCACGCTCGA
AGAGGTACAGGCCGATCTGCCGGAAGAACTCGAAGTCGATCAGGACGAACTGAACGCCGCCCTGGCGCTGTTGCATAGCT
TCGACCCGGCGGGTGTCGGCGCGCGCTCCGCATCCGAATGCCTGAGGCTGCAATTGTTGCGGCTTCCGGGGTCGCCCACG
CGCACACTGGCGTTGGAGATCGTTGCGCATCATCTGGAACTGCTCGCTGCGCGCGACTTCACGCGTCTGCGCAAACACCT
GAAGGCAAGCGACGACGATCTGCGCGATGCGCATGTACTGATTCGCTCGCTCGAACCGTTTCCCGGCGCGGCTTACGGCA
AGGCCGAAGCAGACTACGTGGTGCCCGACATCATGGTGCGCAAAACGGCTCAGGGGTGGCAGGCGGAGCTGAACCCCGAG
GTGGTGCCGAAGCTGCGCATCAATCACCTGTATGCCAATATTCTGCGCAATAACCGGGGGGACCCGGGCAGTGGATCGTT
GCGCCAGCAACTGCAGGAAGCACGCTGGCTGATCAAGAATATCCAGCAGCGTTTCGAGACTATCCTCAGGGTCGCGCAAG
CCATTGTCGAGCGTCAAAAGAGCTTTTTTGTGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATTGCTGAT
ACGCTGGGTTTACACGAGTCAACTGTCTCGCGTGTGACAACCGGTAAATACATGCTGACCCCATTCGGGACGCTTGAATT
CAAGTACTTTTTCGGATCACACGTTTCGACTGACACGGGCGGCGCAGCCTCTTCCACGGCCATTCGCGCGCTCATCAAAC
AACTGATAGGAGCGGAAAACTCAAAATCGCCTCTTTCAGACAGTCGCATAGCCGAACTGCTGGCGGAACAGGGCTTCGTC
GTCGCACGGCGTACCGTCGCGAAGTATCGTGAAGCGCTCAAGATTCCAGCAGTCAACCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(347-502)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-331)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.535

100

0.667

Multiple sequence alignment