Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilK   Type   Machinery gene
Locus tag   T556_RS03145 Genome accession   NZ_CP003974
Coordinates   521021..521632 (+) Length   203 a.a.
NCBI ID   WP_012503481.1    Uniprot ID   B4RKG6
Organism   Neisseria gonorrhoeae NG-k51.05     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 516021..526632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  T556_RS03115 (T556_02820) - 516373..516954 (-) 582 WP_003690895.1 superoxide dismutase -
  T556_RS03125 (T556_02825) dnaB 517118..518521 (+) 1404 WP_012503477.1 replicative DNA helicase -
  T556_RS15065 - 518515..518627 (-) 113 Protein_517 IS5/IS1182 family transposase -
  T556_RS03130 (T556_02830) pilH 518778..519440 (+) 663 WP_041421240.1 GspH/FimT family pseudopilin Machinery gene
  T556_RS03135 (T556_02835) pilV/pilI 519472..520086 (+) 615 WP_012503479.1 type IV pilus modification protein PilV Regulator
  T556_RS03140 (T556_02840) pilJ 520083..521042 (+) 960 WP_012503480.1 PilW family protein Machinery gene
  T556_RS03145 (T556_02845) pilK 521021..521632 (+) 612 WP_012503481.1 pilus assembly PilX family protein Machinery gene
  T556_RS03150 (T556_02850) pilL 521634..522107 (+) 474 WP_012503482.1 PilX family type IV pilin Machinery gene
  T556_RS03155 (T556_02855) - 522177..522485 (-) 309 WP_003706588.1 AzlD family protein -
  T556_RS03160 (T556_02860) - 522482..523193 (-) 712 Protein_524 AzlC family ABC transporter permease -
  T556_RS03165 (T556_02865) dut 523359..523811 (+) 453 WP_003701071.1 dUTP diphosphatase -
  T556_RS03170 (T556_02870) dapC 523883..525070 (+) 1188 WP_003701073.1 succinyldiaminopimelate transaminase -
  T556_RS03175 (T556_02875) yaaA 525226..526005 (+) 780 WP_003687925.1 peroxide stress protein YaaA -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 22021.91 Da        Isoelectric Point: 8.0862

>NTDB_id=99609 T556_RS03145 WP_012503481.1 521021..521632(+) (pilK) [Neisseria gonorrhoeae NG-k51.05]
MRKQNTLTGIPTSDGQRGSALFIVLMVMIVVAFLVVTAAQSYNTEQRISANESDRKLALSLAEAALREGEFQVLDLEYAA
DSKVTFSENCEKGLCTAVNVRTNDNGNEEAFGNIVVQGKPTVEAVKRSCPAKSGKNSTGLCIDNQGVEYKKGTGNVSKMP
RYIIEYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=99609 T556_RS03145 WP_012503481.1 521021..521632(+) (pilK) [Neisseria gonorrhoeae NG-k51.05]
ATGCGCAAACAGAACACTTTGACAGGAATCCCGACTTCTGACGGACAGAGGGGGTCCGCACTGTTTATCGTGCTGATGGT
GATGATAGTCGTGGCCTTTTTGGTTGTAACTGCCGCCCAGTCCTACAATACCGAACAGAGGATCAGTGCCAACGAATCAG
ACAGGAAATTGGCTTTGTCTTTAGCCGAGGCGGCTTTGCGGGAGGGCGAATTTCAGGTTTTGGATTTGGAATATGCTGCG
GACAGTAAGGTTACGTTTAGCGAAAACTGTGAAAAAGGTCTGTGTACCGCAGTGAATGTGCGGACAAATGATAATGGTAA
TGAAGAGGCTTTTGGCAATATCGTGGTGCAAGGCAAGCCCACCGTTGAGGCGGTGAAGCGTTCTTGCCCTGCAAAGTCTG
GCAAAAATTCTACCGGCCTGTGCATTGACAATCAGGGAGTGGAATATAAGAAAGGTACGGGAAACGTCAGCAAAATGCCG
CGCTATATTATCGAATATTTAGGCGTGAAGAACGGACAAAATGTTTATCGGGTTACTGCCAAGGCTTGGGGTAAGAATGC
CAATACCGTGGTCGTCCTTCAATCTTATGTAGGCAATAATGATGAGCAATAA

Domains

Predicted by InterProScan.

PilX N-terminal (PilX_N)

(17-67)

Type IV pilus assembly protein PilX C-term (PilX)

(92-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B4RKG6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilK Neisseria gonorrhoeae MS11

96.059

100

0.961

Multiple sequence alignment