Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AAHT64_RS08100 Genome accession   NZ_CP155054
Coordinates   1547911..1548981 (+) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis subsp. subtilis strain R38     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1542911..1553981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHT64_RS08065 (AAHT64_08065) yqgY 1543115..1543360 (-) 246 WP_003226300.1 DUF2626 domain-containing protein -
  AAHT64_RS08070 (AAHT64_08070) mgsR 1543592..1543972 (+) 381 WP_003230147.1 transcriptional regulator MgsR -
  AAHT64_RS08080 (AAHT64_08080) rsbRD 1544196..1545032 (+) 837 WP_003230149.1 RsbT co-antagonist protein RsbRD -
  AAHT64_RS08085 (AAHT64_08085) yqhB 1545085..1546413 (-) 1329 WP_003230150.1 hemolysin family protein -
  AAHT64_RS08090 (AAHT64_08090) corA 1546556..1547509 (+) 954 WP_032677123.1 magnesium transporter CorA -
  AAHT64_RS08095 (AAHT64_08095) - 1547575..1547700 (+) 126 WP_003230155.1 hypothetical protein -
  AAHT64_RS08100 (AAHT64_08100) comGA 1547911..1548981 (+) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  AAHT64_RS08105 (AAHT64_08105) comGB 1548968..1550005 (+) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  AAHT64_RS08110 (AAHT64_08110) comGC 1550019..1550315 (+) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  AAHT64_RS08115 (AAHT64_08115) comGD 1550305..1550736 (+) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  AAHT64_RS08120 (AAHT64_08120) comGE 1550720..1551067 (+) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  AAHT64_RS08125 (AAHT64_08125) comGF 1551093..1551476 (+) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  AAHT64_RS08130 (AAHT64_08130) comGG 1551477..1551851 (+) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  AAHT64_RS08135 (AAHT64_08135) spoIIT 1551922..1552101 (+) 180 WP_003230176.1 YqzE family protein -
  AAHT64_RS08140 (AAHT64_08140) yqzG 1552143..1552469 (-) 327 WP_003246051.1 YqzG/YhdC family protein -
  AAHT64_RS08145 (AAHT64_08145) tapA 1552741..1553502 (+) 762 WP_003230180.1 amyloid fiber anchoring/assembly protein TapA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=995841 AAHT64_RS08100 WP_004399124.1 1547911..1548981(+) (comGA) [Bacillus subtilis subsp. subtilis strain R38]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=995841 AAHT64_RS08100 WP_004399124.1 1547911..1548981(+) (comGA) [Bacillus subtilis subsp. subtilis strain R38]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment