Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   AAVD42_RS17030 Genome accession   NZ_CP154908
Coordinates   3145718..3149416 (+) Length   1232 a.a.
NCBI ID   WP_345807235.1    Uniprot ID   -
Organism   Bacillus pumilus strain FUA2116     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3140718..3154416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVD42_RS17015 (AAVD42_17015) - 3141013..3141423 (+) 411 WP_106029930.1 DoxX family protein -
  AAVD42_RS17020 (AAVD42_17020) - 3141620..3142066 (-) 447 WP_345807233.1 ferritin-like domain-containing protein -
  AAVD42_RS17025 (AAVD42_17025) addB 3142231..3145740 (+) 3510 WP_345807234.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AAVD42_RS17030 (AAVD42_17030) addA 3145718..3149416 (+) 3699 WP_345807235.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AAVD42_RS17035 (AAVD42_17035) sbcD 3149443..3150618 (+) 1176 WP_106035926.1 exonuclease subunit SbcD -
  AAVD42_RS17040 (AAVD42_17040) sbcC 3150615..3154010 (+) 3396 WP_345807236.1 exonuclease subunit SbcC -
  AAVD42_RS17045 (AAVD42_17045) - 3154030..3154329 (+) 300 WP_044139870.1 hypothetical protein -

Sequence

Protein


Download         Length: 1232 a.a.        Molecular weight: 142131.97 Da        Isoelectric Point: 5.0156

>NTDB_id=995142 AAVD42_RS17030 WP_345807235.1 3145718..3149416(+) (addA) [Bacillus pumilus strain FUA2116]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPALIEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGKHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVVDNPYQ
DIPLAAVLRSPIVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVRALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSVLEETGDVVLSHPSTFTISFTQASDL
LKEDMSLENNEQDEIVQALIDGLPVEGYSDADEQVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESRIGHLLESLQQRDLLTDEQIQSINQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTVDAAEPILKKRYETQ
IALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3699 bp        

>NTDB_id=995142 AAVD42_RS17030 WP_345807235.1 3145718..3149416(+) (addA) [Bacillus pumilus strain FUA2116]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAAATGACCCGGCCTGAACATC
CAGTTGATGTGGACCGTCTGCTCGTTGTGACTTTCACAAATGCTTCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAATCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGATCCAGGCTTTCGTTTGGCTG
ACCAAACAGAAGGAGAACTATTAGGAGATGAAGTGTTAGACGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAATTGGTTGATCGTTACACGTCTGACCGTCACGACTTAGATTTGCAATGGCTCGTGAAGAGAATTTATGAGTT
TTCGAGATCACATCCTTCTCCGGAGCAATGGATGAGGGCATTTCTTTCACTTTATGATGTAGATGCTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAACCGGGCGGCCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTTAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAGCTTTATGAGCTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGACGAATATGACCCTGCTTTAATAGAAAAAGCGACAGATGCACGTAATCAAGCAAAAAAACAATTAGAAAAACTAAAA
GATGAATATTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTGGAGACACTTGTAGA
GCTTGTGATCCAATTTGGTGAGCGTTTCGAAAGAGCGAAGCAGGAAAAGTCAATTGTCGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTACTCGTTGATGAATACCAGGATACAAACCTTGTACAAGAAACGATTTTAAAGCTTGTATCTAAGGGAAA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCTATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTTTTAGATAGTACAAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCACTTAG
ATCAGCAGGAAGCAGAAACCGGCGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGTAACTTACAGTATCGTGACAT
TGTCATTCTGCTTCGGTCTATGCCGTGGGCTCCGCAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCTGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTTTGTTAAAAGTTGTTGATAATCCATATCAG
GATATCCCGCTTGCTGCTGTTTTAAGATCACCTATTGTTCATTTAGACGAAAATGAATTGGCTCTGATTCGAACAAGTGA
TAAAAAAGGCACGTATTATGATGCTGTAAAGGCATTCATGAGTGTGACGCATTCTGATCACCCTACATGCAAAAAACTAG
AGCGATTTTTCCAATTACTGCGCAAGTGGCGTGATTTTTCTATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGTGACACGCAGTATCTTGATTATGTTGGCGGGATGCCTGGGGGTAAGCAGCGGCAGGCGAATCTTCGTGCCCTTTATGA
CAGGGCCAAGCAATACGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GGGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGTATGATGACGATCCATAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACCGCAGGACTCGGCAGAAACTTTAATATGATGGACTTAAACCAGTCTTATTTACTTGA
TAAAGAACTAGGCTTTGGTAGCAAGTATATCCATCCTGAGCTGAGAATTAGTTACGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAATTAAGGGTCCTATATGTAGCGCTCACAAGGGCTAAAGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAGAGCATTAAGCAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
CCCTGATTTTGAACGGTATCAATCGAAAACATACTTAGATTTCATTGGCCCTGCCCTCATCCGTCATCAAGCGATGTCAT
CTGTTTTAGAAGAAACAGGAGATGTTGTTCTTTCGCATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATATGTCACTTGAAAATAATGAGCAAGACGAGATTGTACAAGCATTGATCGATGGTCTGCCTGTTGAAGG
ATATAGTGATGCTGATGAGCAGGTGGCAGAGAGGCTCTCATGGAAATATCCGTATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCAATGCATACAGT
GATGCAGCATATCCCGCTTCCTTCTGAGGAGCCCTATGATGAATCACGTATAGGACACTTGCTCGAATCCTTACAGCAGC
GAGACTTGCTAACAGATGAGCAGATCCAGTCAATAAATCAAGAGGGAATTGCTGCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTAAAAGCGGACTGGGTGAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGTCATAT
TGATACTGAGGGAGAGCCTGTTCTGATTCAAGGGATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAAACTGATAGAGTACAAGGCAGATATACAGTTGACGCTGCTGAACCGATCTTGAAAAAGCGTTATGAAACCCAA
ATAGCGTTATATGCAAAAGCTGTAGAACGTTTGACAAACAGAACATTAGAGGAGAAAATTCTCTATTTCTTCGATGGGAA
TTTAGAAATTTCTTTATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1224)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.1

100

0.663

Multiple sequence alignment