Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AAVD42_RS17025 Genome accession   NZ_CP154908
Coordinates   3142231..3145740 (+) Length   1169 a.a.
NCBI ID   WP_345807234.1    Uniprot ID   -
Organism   Bacillus pumilus strain FUA2116     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3137231..3150740
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVD42_RS16995 (AAVD42_16995) lepB 3137741..3138253 (+) 513 WP_041815362.1 signal peptidase I -
  AAVD42_RS17000 (AAVD42_17000) - 3138290..3139435 (-) 1146 WP_345808465.1 AbrB family transcriptional regulator -
  AAVD42_RS17005 (AAVD42_17005) - 3139702..3140124 (+) 423 WP_345807231.1 BlaI/MecI/CopY family transcriptional regulator -
  AAVD42_RS17010 (AAVD42_17010) - 3140117..3140956 (+) 840 WP_345807232.1 M56 family metallopeptidase -
  AAVD42_RS17015 (AAVD42_17015) - 3141013..3141423 (+) 411 WP_106029930.1 DoxX family protein -
  AAVD42_RS17020 (AAVD42_17020) - 3141620..3142066 (-) 447 WP_345807233.1 ferritin-like domain-containing protein -
  AAVD42_RS17025 (AAVD42_17025) addB 3142231..3145740 (+) 3510 WP_345807234.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AAVD42_RS17030 (AAVD42_17030) addA 3145718..3149416 (+) 3699 WP_345807235.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AAVD42_RS17035 (AAVD42_17035) sbcD 3149443..3150618 (+) 1176 WP_106035926.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134785.70 Da        Isoelectric Point: 5.3483

>NTDB_id=995141 AAVD42_RS17025 WP_345807234.1 3142231..3145740(+) (addB) [Bacillus pumilus strain FUA2116]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLAQQIPSAEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAAKITAAFTVDRS
YHDRQPNELDLFRMTGKTYFQLYQLAKECGADISENIFERNHRHLYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSANK
RAEIEGVARDILDLVREKGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPVEITKNKAREHADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IVPPLYQLQKRLKKAQKVRDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVDMLGDEEL
SFPLFQQMIDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSRHLYLSYAASDAEGRSLLPSTLIKYCQELMPNHQQALYVLDPEQLEDDEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSPHRDVSKNIMSSLFFTNRAKPLKDNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKERQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVNYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKLSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLNSGRSQVIQAGLKKDGSFRSDSAVLSEDHFQILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIERIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=995141 AAVD42_RS17025 WP_345807234.1 3142231..3145740(+) (addB) [Bacillus pumilus strain FUA2116]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCGAAAA
CGTCTGAAGCTGGTGGAATGATCAGAGCTAAAGTATTTAGTTTCACTCGTCTTGCTTGGTCTATTTTACAGCAGACTGGT
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAAACGATGGCCGAATTCAAAAGATACT
GTATGCTGCCGGAGGACATTGAGAAAATTTCAGCGTCTAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTCCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACTCTTCTTGCCCAGCAAATTCCTTCAGCTGAAGAAATAAAAGGAGCACACATCTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTTGTCATTGAACAGCTCTTGCTGCATGCAGCAAAAATAACTGCGGCTTTCACAGTAGATCGATCC
TATCATGATAGGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCTAA
AGAGTGCGGAGCTGACATTTCTGAAAACATTTTTGAAAGAAATCATCGGCACCTTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGCGGCCCGTGCAGCCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGATATTCTTGATTTAGTGAGAGAAAAAGGACTCAGATTGCGAGATATCTCGAT
TGTAGCGCGGCATGTAGATGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTAATTCGTTCGAGCTTGGATGTCATAAAAGGAAATTGGCGGTAT
GAAGCGGTATTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGGTTGAAATCACTAAGAACAAAGCGAGAGAGCATGCAGA
TCAGCTTGAAAACTATTGTATTGCTTATGGTGTAAAAGGAGAGCGCTGGACAAACGGTTCCCGGTTTCATTACAGGCGTT
TCCAATCATTAGATGAGGATTTCGGACAAACAGATCAAGAAATTGAAATGGAACAAATGCTAAATGACGTCAAAGAATGG
ATCGTGCCCCCGCTTTACCAGTTGCAGAAAAGGCTCAAAAAAGCGCAAAAGGTAAGGGACATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAGCTTGAAAAAGCGAGGCTTGAGGCTGAAGAAGCAGGTCGATTAGCTGAAG
CAATGCAGCACGGGCAAGTATGGGATGCGGTGATTCAATTAATGGATGAATTTGTTGACATGCTAGGTGACGAAGAGCTC
TCATTTCCTTTATTCCAACAGATGATAGATACAGGATTAGCTTCTCTAAAATTCGCTTTGATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTATGTTTATCATTGGTGTAAATGATGGCGTTA
TTCCCGCACGCCCCTCTGATGAGAGTGTATTGTCTGAGGATGACAGAGAATGGTTAAAGCGAGCAGGAGCAGAGCTAGCA
GAGACGGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCGACATTTGTATCT
ATCCTATGCGGCTTCTGATGCAGAAGGACGTTCTCTATTGCCTTCGACACTCATCAAGTATTGCCAAGAGCTTATGCCAA
ATCATCAGCAGGCTCTTTATGTGTTAGATCCAGAACAGCTGGAAGACGATGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTGTCGTATACCGTCTCACAGCTGCAGCAATGGCTCAATCAATATCCCATTAGCGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTCATCGGGATGTATCAAAAAACATCATGTCAAGTTTGTTCTTTACAAATCGAGCGAAGC
CTTTAAAGGATAATGTCACAAAAGAGCTTTACGGTGATCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGTTTAAAACTAAAGGAACGACAATTTTACAAATTGGAAGCACCCGATATTGG
CCAGTTGTTTCACTCGGCTTTGAAGCATATTTCAGATACGCTAGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGATTGTGTAAACTACTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAGATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTTCAGAAAATCTTAATCCGAGTCTCTTCTATATTAAGTGAACATGCCAAATTGAG
TGGATTTTCTCCAGTAGGGCTTGAGTTAGGTTTTGGGGGTCAAGGGCCTTTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGTACGATGGAGCTTGTCGGAAGAATTGACAGGGTAGATAAAGCAGAAGGTTCAAAAGGGCTGCTTTTAAGAATCGTC
GATTACAAATCCAGTGAGAAAGGTCTTGACCTAGCAGAAGTATACTATGGTCTTGCCTTGCAAATGCTCACATACTTAGA
CCTAACCATTACGTATTCGAAAGAGTGGCTCGGCGTAGAAGCAACGCCTGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCACTTGCAGAGGATGAAATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAGGGTTTA
TTGCTTGAAGATGTGGAAGCAGTAAAGCTAATGGATCAAACGCTTAATTCAGGTAGATCGCAAGTCATTCAAGCCGGTTT
GAAAAAGGATGGGTCTTTCCGTTCTGATTCGGCAGTTTTAAGTGAAGATCATTTCCAAATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGAGAGGTCGCAATTAATCCATATAAATTAAAGGATCAAACACCT
TGCCGCTTTTGTTCATTCAAGTCTATTTGCCAATTCGATGAATCAATAGAAGATAATGATTTTAGGGTGCTCACCTCTGA
AAAGGATGATGTTGTGATAGAACGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.408

99.316

0.66

Multiple sequence alignment