Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AAVB98_RS07395 Genome accession   NZ_CP154895
Coordinates   1404718..1405155 (+) Length   145 a.a.
NCBI ID   WP_013350968.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Fad 77     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1399718..1410155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVB98_RS07380 (AAVB98_07380) - 1402584..1402946 (-) 363 WP_013350971.1 helix-turn-helix domain-containing protein -
  AAVB98_RS07385 (AAVB98_07385) hxlA 1403178..1403813 (+) 636 WP_013350970.1 3-hexulose-6-phosphate synthase -
  AAVB98_RS07390 (AAVB98_07390) hxlB 1403810..1404367 (+) 558 WP_013350969.1 6-phospho-3-hexuloisomerase -
  AAVB98_RS07395 (AAVB98_07395) nucA/comI 1404718..1405155 (+) 438 WP_013350968.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  AAVB98_RS07400 (AAVB98_07400) nin/comJ 1405176..1405574 (+) 399 WP_061573682.1 competence protein ComJ Regulator
  AAVB98_RS07405 (AAVB98_07405) - 1405615..1407051 (-) 1437 WP_013350966.1 family 1 glycosylhydrolase -
  AAVB98_RS07410 (AAVB98_07410) - 1407156..1407479 (-) 324 WP_013350965.1 YckD family protein -
  AAVB98_RS07415 (AAVB98_07415) - 1407553..1407987 (-) 435 WP_013350964.1 RDD family protein -
  AAVB98_RS07420 (AAVB98_07420) - 1408002..1409133 (-) 1132 Protein_1448 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=994767 AAVB98_RS07395 WP_013350968.1 1404718..1405155(+) (nucA/comI) [Bacillus amyloliquefaciens strain Fad 77]
MKTLKALLLVLIIIAGAVAALIKGDIFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=994767 AAVB98_RS07395 WP_013350968.1 1404718..1405155(+) (nucA/comI) [Bacillus amyloliquefaciens strain Fad 77]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATATTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAACAATCTCTTAAACATGTGCCTGTAAAAACAGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCAGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment