Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AAVD18_RS18320 Genome accession   NZ_CP154893
Coordinates   3485411..3485848 (-) Length   145 a.a.
NCBI ID   WP_013350968.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Fad 11/2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3480411..3490848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVD18_RS18295 (AAVD18_18295) - 3481433..3482564 (+) 1132 Protein_3554 zinc-dependent alcohol dehydrogenase -
  AAVD18_RS18300 (AAVD18_18300) - 3482579..3483013 (+) 435 WP_013350964.1 RDD family protein -
  AAVD18_RS18305 (AAVD18_18305) - 3483087..3483410 (+) 324 WP_013350965.1 YckD family protein -
  AAVD18_RS18310 (AAVD18_18310) - 3483515..3484951 (+) 1437 WP_013350966.1 family 1 glycosylhydrolase -
  AAVD18_RS18315 (AAVD18_18315) nin/comJ 3484992..3485390 (-) 399 WP_061573682.1 competence protein ComJ Regulator
  AAVD18_RS18320 (AAVD18_18320) nucA/comI 3485411..3485848 (-) 438 WP_013350968.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  AAVD18_RS18325 (AAVD18_18325) hxlB 3486199..3486756 (-) 558 WP_013350969.1 6-phospho-3-hexuloisomerase -
  AAVD18_RS18330 (AAVD18_18330) hxlA 3486753..3487388 (-) 636 WP_013350970.1 3-hexulose-6-phosphate synthase -
  AAVD18_RS18335 (AAVD18_18335) - 3487620..3487982 (+) 363 WP_013350971.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=994736 AAVD18_RS18320 WP_013350968.1 3485411..3485848(-) (nucA/comI) [Bacillus amyloliquefaciens strain Fad 11/2]
MKTLKALLLVLIIIAGAVAALIKGDIFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=994736 AAVD18_RS18320 WP_013350968.1 3485411..3485848(-) (nucA/comI) [Bacillus amyloliquefaciens strain Fad 11/2]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATATTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAACAATCTCTTAAACATGTGCCTGTAAAAACAGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCAGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment