Detailed information NTG0994443

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: AAYT23_RS04220
• Genome accession: NZ_CP154877
• Coordinates: 828801..829451 (+)
• Length: 216 a.a.
• NCBI ID: WP_000387324.1
• Uniprot ID: A0A6I3V3S0
• Organism: Streptococcus pneumoniae strain 20234295
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 823801..834451

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAYT23_RS04190 (AAYT23_04175)	-	824285..824500 (+)	216	WP_001232082.1	YozE family protein	-
AAYT23_RS04195 (AAYT23_04180)	-	824586..825554 (+)	969	WP_000658183.1	PhoH family protein	-
AAYT23_RS04200 (AAYT23_04185)	-	825747..826247 (+)	501	WP_000566988.1	GNAT family N-acetyltransferase	-
AAYT23_RS04205 (AAYT23_04190)	-	826250..826576 (+)	327	Protein_828	TfoX/Sxy family protein	-
AAYT23_RS04210 (AAYT23_04195)	ald	826877..827988 (-)	1112	Protein_829	alanine dehydrogenase	-
AAYT23_RS04215 (AAYT23_04200)	-	828165..828734 (+)	570	WP_000443743.1	GNAT family N-acetyltransferase	-
AAYT23_RS04220 (AAYT23_04205)	comEA/celA/cilE	828801..829451 (+)	651	WP_000387324.1	helix-hairpin-helix domain-containing protein	Machinery gene
AAYT23_RS04225 (AAYT23_04210)	comEC/celB	829435..831675 (+)	2241	WP_000942413.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AAYT23_RS04230 (AAYT23_04215)	-	831854..832042 (+)	189	WP_001809102.1	hypothetical protein	-
AAYT23_RS04235 (AAYT23_04220)	-	832074..832661 (+)	588	WP_000933542.1	ATP-binding cassette domain-containing protein	-
AAYT23_RS04240 (AAYT23_04225)	-	832665..833846 (+)	1182	WP_000655952.1	membrane protein	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23243.68 Da
• Isoelectric Point: 5.5171

>NTDB_id=994443 AAYT23_RS04220 WP_000387324.1 828801..829451(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain 20234295]
MEAIIEKIKEYKIIVICTGLGLIVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=994443 AAYT23_RS04220 WP_000387324.1 828801..829451(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain 20234295]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGATTGTAGGAGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAGACGAATTTGCAGGCTGAAGTCGCAGCTGTTTCCAAGGATT
TGGTATCCGAAAAGGAAGTGAACAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGCCAACAGACTGGTTTGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCT
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGGCAAATGG
CAAGTTCAAATCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGCCAAGACTATAGAAAAGTTAAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-214)

• SLBB domain (SLBB) (76-126)

3D structure

• AlphaFold DB: A0A6I3V3S0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae Rx1	99.074	100	0.991
comEA/celA/cilE	Streptococcus pneumoniae D39	99.074	100	0.991
comEA/celA/cilE	Streptococcus pneumoniae R6	99.074	100	0.991
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	96.296	100	0.963
comEA/celA/cilE	Streptococcus mitis NCTC 12261	96.296	100	0.963
comEA/celA/cilE	Streptococcus mitis SK321	90.278	100	0.903
comEA	Lactococcus lactis subsp. cremoris KW2	43.612	100	0.458
comEA	Bacillus subtilis subsp. subtilis str. 168	41.579	87.963	0.366
comEA	Latilactobacillus sakei subsp. sakei 23K	33.193	100	0.366