Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AAYT22_RS00145 Genome accession   NZ_CP154876
Coordinates   25754..27115 (+) Length   453 a.a.
NCBI ID   WP_078143130.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 20155336     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 20754..32115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAYT22_RS00110 (AAYT22_00110) - 21110..21907 (-) 798 Protein_15 transposase -
  AAYT22_RS00115 (AAYT22_00115) comW 22172..22408 (+) 237 WP_000939546.1 sigma(X)-activator ComW Regulator
  AAYT22_RS00120 (AAYT22_00120) - 22639..23925 (+) 1287 WP_001819018.1 adenylosuccinate synthase -
  AAYT22_RS00125 (AAYT22_00125) tadA 24126..24593 (+) 468 WP_000291875.1 tRNA adenosine(34) deaminase TadA -
  AAYT22_RS00135 (AAYT22_00135) - 24780..25223 (+) 444 WP_000701974.1 dUTP diphosphatase -
  AAYT22_RS00140 (AAYT22_00140) - 25225..25740 (+) 516 WP_000691236.1 histidine phosphatase family protein -
  AAYT22_RS00145 (AAYT22_00145) radA 25754..27115 (+) 1362 WP_078143130.1 DNA repair protein RadA Machinery gene
  AAYT22_RS00150 (AAYT22_00150) - 27188..27685 (+) 498 WP_001809263.1 beta-class carbonic anhydrase -
  AAYT22_RS00155 (AAYT22_00155) - 27710..28525 (+) 816 WP_000749768.1 PrsW family intramembrane metalloprotease -
  AAYT22_RS00160 (AAYT22_00160) - 28670..29638 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  AAYT22_RS00165 (AAYT22_00165) - 29772..30053 (-) 282 Protein_25 ISL3 family transposase -
  AAYT22_RS00170 (AAYT22_00170) - 30180..31087 (-) 908 Protein_26 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49502.06 Da        Isoelectric Point: 6.8564

>NTDB_id=994345 AAYT22_RS00145 WP_078143130.1 25754..27115(+) (radA) [Streptococcus pneumoniae strain 20155336]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDREFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=994345 AAYT22_RS00145 WP_078143130.1 25754..27115(+) (radA) [Streptococcus pneumoniae strain 20155336]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGAGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTGCGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACAGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGAGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCGATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(63-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

99.779

100

0.998
  radA Streptococcus pneumoniae D39

99.779

100

0.998
  radA Streptococcus pneumoniae R6

99.779

100

0.998
  radA Streptococcus pneumoniae TIGR4

99.779

100

0.998
  radA Streptococcus mitis NCTC 12261

99.338

100

0.993
  radA Streptococcus mitis SK321

99.338

100

0.993
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment