Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   AAG092_RS07585 Genome accession   NZ_CP154874
Coordinates   1557708..1559300 (+) Length   530 a.a.
NCBI ID   WP_373389193.1    Uniprot ID   -
Organism   Pseudomonas alcaligenes strain Med1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1552708..1564300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG092_RS07565 (AAG092_07515) pgeF 1554507..1555241 (-) 735 WP_373389189.1 peptidoglycan editing factor PgeF -
  AAG092_RS07570 (AAG092_07520) rluD 1555225..1556196 (-) 972 WP_373389190.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  AAG092_RS07575 (AAG092_07525) - 1556344..1557366 (+) 1023 WP_373389191.1 outer membrane protein assembly factor BamD -
  AAG092_RS07580 (AAG092_07530) - 1557488..1557718 (+) 231 WP_373389192.1 PP0621 family protein -
  AAG092_RS07585 (AAG092_07535) pilS 1557708..1559300 (+) 1593 WP_373389193.1 PAS domain-containing sensor histidine kinase Regulator
  AAG092_RS07590 (AAG092_07540) pilR 1559303..1560643 (+) 1341 WP_373389194.1 sigma-54-dependent transcriptional regulator Regulator
  AAG092_RS07595 (AAG092_07545) - 1560692..1561762 (-) 1071 WP_373389195.1 NAD(P)/FAD-dependent oxidoreductase -
  AAG092_RS07600 (AAG092_07550) - 1561876..1562385 (+) 510 WP_373389196.1 GspH/FimT family pseudopilin -
  AAG092_RS07605 (AAG092_07555) - 1562491..1562982 (+) 492 WP_110681358.1 GspH/FimT family pseudopilin -
  AAG092_RS07610 (AAG092_07560) pilV 1562979..1563491 (+) 513 WP_373389197.1 type IV pilus modification protein PilV -
  AAG092_RS07615 (AAG092_07565) - 1563491..1564258 (+) 768 WP_373389198.1 PilW family protein -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58952.33 Da        Isoelectric Point: 9.4018

>NTDB_id=994323 AAG092_RS07585 WP_373389193.1 1557708..1559300(+) (pilS) [Pseudomonas alcaligenes strain Med1]
MTADALTLGELPGRRILRLYNLYRVTVGLALVLLISSQLDQELLQLAHVQLFRYGSWAYLIFNVLVAVFMQRPRHLAQVF
GLALLDVLLLCALFYSAGGTPSGIGNLLVVAVAIANILLRGRIGLLIAAVAAIGMLSLTFYLSLTRSAATAQFVQAGALG
ALCFAAALFLQGLTRRLQQSESLAEQRAVDVASLEALNSLILQRMRTGILVLDKQHRVLLANQGALGMLGRGKLEGKVLA
PYCPELVRRLQQWQHNPTLRPQSLQAIPEGPVLQPSFIPLQRGEEQHTLVFLDDISQITQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESEALDDPDRRLAQIIQDHSRRMNMVIENVLQLSRRRQAEPQLLDLKYWLHRFASEFRNSARID
QTLHLETLGSGLQTRMDPHQLNQVLTNLVQNGLRYSAQKHQLGQVWLKLYRDSESDLPTLEVLDNGPGVPPDQVQHIFEP
FYTTDNKGTGLGLYISRELCESNQARLDYKPREGGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=994323 AAG092_RS07585 WP_373389193.1 1557708..1559300(+) (pilS) [Pseudomonas alcaligenes strain Med1]
GTGACCGCTGACGCCCTGACCCTCGGCGAGCTGCCCGGCCGCCGCATCCTGCGCCTGTACAACCTGTACCGGGTCACCGT
CGGCCTGGCCCTGGTATTGCTGATCTCCAGCCAGCTGGATCAGGAACTGCTGCAGCTGGCCCACGTCCAGCTGTTCCGCT
ACGGCAGCTGGGCCTACCTGATCTTCAACGTGCTGGTCGCCGTGTTCATGCAGCGTCCACGGCACCTGGCCCAGGTGTTC
GGCCTGGCACTGCTCGACGTGCTGCTGCTGTGCGCCCTGTTCTACTCGGCCGGCGGCACCCCCAGCGGCATCGGCAACCT
GCTGGTGGTGGCGGTGGCCATCGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGCTGATCGCCGCCGTCGCCGCCATCG
GCATGCTCAGCCTGACCTTCTACCTGAGCCTGACCCGTTCGGCCGCCACCGCCCAATTCGTCCAGGCCGGCGCCCTCGGC
GCCCTGTGCTTCGCCGCGGCGCTGTTCCTCCAGGGCCTGACCCGGCGCCTGCAGCAGAGCGAGAGCCTGGCCGAGCAGCG
CGCGGTCGACGTGGCCAGCCTGGAGGCGCTCAACTCGCTGATCCTGCAGCGCATGCGCACCGGCATCCTGGTGCTGGACA
AGCAGCACCGGGTGCTGCTGGCCAACCAGGGCGCCCTCGGCATGCTCGGCCGCGGCAAGCTCGAGGGCAAGGTGCTCGCC
CCATACTGCCCGGAGCTGGTCAGGCGCCTGCAACAGTGGCAGCACAACCCCACCCTGCGCCCGCAGAGCCTGCAGGCCAT
ACCGGAGGGGCCGGTGCTGCAGCCGAGCTTCATCCCCCTGCAGCGCGGCGAGGAGCAGCACACCCTGGTCTTCCTCGACG
ATATCTCGCAGATCACCCAGCAGGCCCAGCAGCTCAAGCTGGCCTCCCTCGGCCGGCTGACCGCCGGCATCGCCCACGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCATGCCGCGCAGCTGCTGCAGGAGTCCGAGGCCCTGGACGATCCGGACCGGCG
CCTGGCGCAGATCATCCAGGACCATTCGCGGCGGATGAACATGGTCATCGAGAACGTCCTGCAGCTGTCGCGCCGGCGCC
AGGCCGAGCCGCAGCTGCTCGACCTGAAGTACTGGCTGCACCGCTTCGCCAGCGAATTCCGCAACTCGGCGCGGATCGAC
CAGACCCTGCACCTGGAGACGCTCGGCAGCGGCCTGCAGACGCGCATGGACCCGCATCAGCTGAACCAGGTGCTGACCAA
TCTGGTGCAGAACGGCCTGCGCTACAGCGCGCAGAAACACCAGCTCGGCCAGGTCTGGCTGAAGCTGTATCGCGATAGCG
AGAGCGACCTGCCGACCCTGGAAGTGCTGGATAACGGCCCCGGCGTGCCACCCGACCAGGTACAGCACATCTTCGAACCC
TTCTACACCACCGACAACAAGGGCACCGGTCTGGGCCTGTACATCTCCCGCGAGCTGTGCGAAAGCAATCAGGCACGCCT
CGACTACAAACCGCGCGAAGGTGGCGGCAGCTGCTTCCGCATCACCTTCGCCCACCCACGCAAGCTGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

67.358

100

0.674

Multiple sequence alignment