Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAG895_RS02840 Genome accession   NZ_CP154859
Coordinates   646297..647754 (-) Length   485 a.a.
NCBI ID   WP_345794053.1    Uniprot ID   -
Organism   Thauera sp. JM12B12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 641297..652754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG895_RS02815 (AAG895_02815) - 642177..642770 (+) 594 WP_345794048.1 5-formyltetrahydrofolate cyclo-ligase -
  AAG895_RS02820 (AAG895_02820) ilvA 642789..644312 (-) 1524 WP_345794049.1 threonine ammonia-lyase, biosynthetic -
  AAG895_RS02825 (AAG895_02825) hprK 644415..645362 (-) 948 WP_345794050.1 HPr(Ser) kinase/phosphatase -
  AAG895_RS02830 (AAG895_02830) ptsN 645331..645816 (-) 486 WP_345794051.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AAG895_RS02835 (AAG895_02835) raiA 645956..646279 (-) 324 WP_345794052.1 ribosome-associated translation inhibitor RaiA -
  AAG895_RS02840 (AAG895_02840) rpoN/rpoN1 646297..647754 (-) 1458 WP_345794053.1 RNA polymerase factor sigma-54 Machinery gene
  AAG895_RS02845 (AAG895_02845) lptB 647777..648499 (-) 723 WP_345794054.1 LPS export ABC transporter ATP-binding protein -
  AAG895_RS02850 (AAG895_02850) recX 648486..648935 (-) 450 WP_345794055.1 recombination regulator RecX -
  AAG895_RS02855 (AAG895_02855) recA 648952..649989 (-) 1038 WP_345794056.1 recombinase RecA Machinery gene
  AAG895_RS02860 (AAG895_02860) - 650185..650682 (-) 498 WP_345794057.1 nicotinamide-nucleotide amidohydrolase family protein -
  AAG895_RS02865 (AAG895_02865) - 650687..651169 (-) 483 WP_345794058.1 phosphatidylglycerophosphatase A -
  AAG895_RS02870 (AAG895_02870) thiL 651235..652194 (-) 960 WP_345794059.1 thiamine-phosphate kinase -

Sequence

Protein


Download         Length: 485 a.a.        Molecular weight: 53690.34 Da        Isoelectric Point: 4.9490

>NTDB_id=994145 AAG895_RS02840 WP_345794053.1 646297..647754(-) (rpoN/rpoN1) [Thauera sp. JM12B12]
MKPSLQLKLSQHLTLTPQLQQSIKLLQLSTIELNQEIERILLENPMLEREDGDGADFAPSPPPTPSSERESSSESAAESP
PADSASREGGEGSSEDEGIDWSNVGTGASSRSESDDDDVDYQDLQAASTSLREHLDQQVALSPLSDRDRALVRFLIEALD
DDGYLHQPLEDLLQLLPPESEVDLDELTIALHHVHNLEPAGIGARSPQECLALQLRTLPDSPIRALALDIATRHLELLAD
RNFARIKKLTGCDDDALRAAQALICSLDPHPGSRYSNTETRYVLPDVVVRKLRGRWTVTLNHDAMPRLRINRLYASLLQQ
NRGQGGGLGGQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGDVAMRPLTLREIADQLELHESTVSRVTTQKYMA
TPRGVFELKYFFGSHVATDTGGAASSTAIRALIRQLVDAEDSKKPLSDAKIAEVLGQQGIVVARRTIAKYRESLNIPPVS
LRKSI

Nucleotide


Download         Length: 1458 bp        

>NTDB_id=994145 AAG895_RS02840 WP_345794053.1 646297..647754(-) (rpoN/rpoN1) [Thauera sp. JM12B12]
ATGAAGCCCAGTCTCCAGCTCAAGCTCTCCCAGCACCTCACGCTGACCCCGCAGCTGCAGCAGTCGATCAAGCTGCTGCA
GCTGTCGACGATCGAGCTCAACCAGGAGATCGAGCGCATCCTGCTCGAGAACCCGATGCTCGAACGCGAGGACGGCGACG
GCGCGGACTTCGCTCCGAGCCCGCCCCCCACACCCTCGTCCGAACGCGAGTCGAGCAGCGAGAGCGCCGCCGAATCGCCC
CCGGCCGACAGCGCGTCGCGCGAGGGCGGCGAGGGCAGCAGCGAGGACGAAGGCATCGACTGGTCCAACGTCGGCACCGG
CGCATCCAGCCGCAGCGAGAGCGACGACGACGATGTCGACTACCAGGATCTGCAGGCCGCGAGCACATCCCTGCGCGAAC
ACCTCGACCAGCAGGTGGCGCTGTCGCCGCTCAGCGACCGCGACCGCGCCCTGGTGCGCTTCCTGATCGAGGCACTCGAT
GACGACGGCTACCTGCACCAGCCGCTCGAGGACCTGCTCCAACTGCTGCCGCCCGAGTCCGAGGTCGATCTCGACGAGCT
CACCATCGCCCTCCACCACGTGCACAACCTCGAGCCCGCCGGCATCGGCGCCCGCAGCCCGCAGGAATGCCTGGCGCTGC
AGCTGCGCACGCTGCCCGACTCGCCGATCCGCGCGCTCGCCCTCGACATCGCCACGCGGCACCTCGAGCTTCTCGCCGAC
CGCAACTTCGCCCGCATCAAGAAGCTCACCGGCTGCGACGACGACGCCCTGCGTGCCGCGCAGGCGCTGATCTGCAGCCT
CGACCCCCACCCCGGCTCGCGCTACTCGAACACCGAGACGCGCTACGTGCTGCCCGACGTCGTCGTGCGCAAGCTGCGCG
GGCGGTGGACGGTCACGCTCAACCACGACGCCATGCCGCGCCTGCGCATCAACCGCCTGTACGCCAGCCTGCTGCAGCAG
AACCGCGGCCAGGGCGGCGGACTCGGCGGCCAGCTGCAGGAGGCACGCTGGCTGATCAAGAACGTGCAGCAGCGTTTCGA
CACCATCCTGCGCGTATCCCAGGCCATCGTTGACCAGCAACGGCAGTTCTTCGATCATGGGGACGTGGCGATGCGGCCAC
TCACCCTCCGGGAGATCGCGGACCAGCTCGAGCTGCATGAATCCACCGTCTCGCGCGTCACCACGCAGAAGTACATGGCG
ACCCCGCGTGGCGTGTTCGAACTCAAGTATTTCTTCGGCAGTCATGTCGCCACCGACACCGGTGGAGCTGCATCCTCGAC
GGCGATTCGGGCGCTGATCCGCCAGCTGGTGGATGCCGAAGACAGCAAGAAGCCGCTGTCCGACGCCAAGATTGCGGAAG
TTTTGGGCCAGCAGGGTATCGTGGTCGCGCGGCGGACGATCGCCAAATACCGCGAGTCCCTCAATATCCCGCCGGTCAGT
CTGCGCAAATCCATCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(121-314)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(331-483)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.685

100

0.596

Multiple sequence alignment