Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   AAEQ75_RS03130 Genome accession   NZ_CP154631
Coordinates   642479..644071 (+) Length   530 a.a.
NCBI ID   WP_343352318.1    Uniprot ID   -
Organism   Pseudomonas sediminis strain I4     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 637479..649071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEQ75_RS03105 (AAEQ75_03105) - 638504..639094 (-) 591 WP_343350861.1 TetR/AcrR family transcriptional regulator -
  AAEQ75_RS03110 (AAEQ75_03110) pgeF 639227..639958 (-) 732 WP_343350862.1 peptidoglycan editing factor PgeF -
  AAEQ75_RS03115 (AAEQ75_03115) rluD 639955..640929 (-) 975 WP_343350863.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  AAEQ75_RS03120 (AAEQ75_03120) - 641068..642060 (+) 993 WP_179575669.1 outer membrane protein assembly factor BamD -
  AAEQ75_RS03125 (AAEQ75_03125) - 642253..642489 (+) 237 WP_099522406.1 PP0621 family protein -
  AAEQ75_RS03130 (AAEQ75_03130) pilS 642479..644071 (+) 1593 WP_343352318.1 ATP-binding protein Regulator
  AAEQ75_RS03135 (AAEQ75_03135) pilR 644074..645414 (+) 1341 WP_343350864.1 sigma-54 dependent transcriptional regulator Regulator
  AAEQ75_RS03140 (AAEQ75_03140) thiO 645506..646600 (-) 1095 WP_343350865.1 glycine oxidase ThiO -
  AAEQ75_RS03145 (AAEQ75_03145) - 646691..647200 (+) 510 WP_343350866.1 GspH/FimT family pseudopilin -
  AAEQ75_RS03150 (AAEQ75_03150) - 647310..647801 (+) 492 WP_143506889.1 GspH/FimT family pseudopilin -
  AAEQ75_RS03155 (AAEQ75_03155) pilV 647798..648316 (+) 519 WP_343350867.1 type IV pilus modification protein PilV -
  AAEQ75_RS03160 (AAEQ75_03160) - 648319..649041 (+) 723 WP_343350868.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59066.23 Da        Isoelectric Point: 8.4724

>NTDB_id=993843 AAEQ75_RS03130 WP_343352318.1 642479..644071(+) (pilS) [Pseudomonas sediminis strain I4]
MIADTSVQNSKQGQRILRLYHFYRLSIGLALILLISSNLDEQLLDVVHIELFRTGSWVYLILNIFIAVLVQKPKHLVQLF
SLALVDVIFLSALFYAAGGTPSGIGNLLVVAVAIANILLRGRIGLLIAAIAAMALIYLTFYLSLSRPAAAAQYVQAGALG
ALCFAGALFIQGITRRLQVSESLAEQRAADVANLEELNEQILQRMRTGILVLDTQHRVLLTNQGAKQLLGRGELTGKIID
PHCPELVKRLQQWLHNPTLRPDSLQAQVDGPVLQPSFISLQRGDQQHTLVFLDDISQIAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESEDLHGPDQRLAQIIQDHSKRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFASEFRSSAPAD
RQLHLEARGGGLQTRMDPHQLNQVLTNLVQNGLRYSAQKHRLGQVWLRLFRDAESDLPVLEILDDGPGVPPEQEPHIFEP
FYTTENKGTGLGLYISRELCESNQARLDYKPREGGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=993843 AAEQ75_RS03130 WP_343352318.1 642479..644071(+) (pilS) [Pseudomonas sediminis strain I4]
GTGATCGCTGACACCAGCGTACAGAACAGCAAACAAGGGCAGCGCATCCTCAGGCTCTACCACTTCTATCGCCTGAGCAT
CGGCCTGGCGCTGATCCTGCTAATTTCCAGCAACCTCGACGAGCAGTTGCTGGACGTCGTGCATATCGAGCTATTTCGCA
CCGGCAGCTGGGTCTACCTGATCCTCAACATCTTCATCGCCGTGCTGGTGCAAAAGCCCAAGCACCTGGTGCAGCTGTTC
AGCCTGGCGCTTGTCGACGTGATCTTTCTCTCGGCCCTGTTCTATGCAGCCGGCGGCACGCCCAGCGGCATCGGCAACCT
GCTGGTGGTCGCCGTGGCCATCGCCAATATCCTGCTGCGCGGCCGCATCGGCCTGCTGATCGCCGCCATTGCCGCGATGG
CGCTGATCTACCTGACCTTCTATCTCAGCCTCAGCCGCCCCGCCGCCGCCGCGCAGTACGTACAAGCAGGCGCACTCGGT
GCGTTGTGCTTCGCCGGCGCTCTGTTCATCCAGGGCATCACACGCCGCCTGCAGGTAAGCGAGAGCCTGGCCGAACAACG
TGCGGCCGATGTAGCCAACCTCGAAGAGCTCAACGAGCAGATCCTGCAGCGTATGCGCACCGGTATCCTCGTGCTCGACA
CCCAACATCGGGTGCTGCTGACCAACCAGGGCGCAAAGCAGTTGCTCGGGCGCGGTGAACTGACCGGAAAGATCATCGAT
CCGCACTGCCCCGAACTGGTCAAGCGTCTGCAGCAGTGGCTGCACAACCCAACCCTGCGCCCGGACAGCCTGCAGGCCCA
GGTCGACGGGCCGGTGCTACAGCCCAGCTTCATTTCCCTTCAGCGCGGTGACCAGCAGCACACCCTGGTGTTTCTCGATG
ACATCTCGCAGATCGCCCAGCAGGCGCAGCAGCTCAAGCTGGCCTCGCTCGGCCGCCTGACCGCAGGCATCGCTCACGAA
ATTCGTAATCCGCTCGGCGCCATCAGCCACGCAGCGCAGCTACTGCAGGAATCGGAGGATTTGCACGGCCCGGATCAGCG
CCTGGCGCAGATCATCCAGGACCACTCCAAACGCATGAACCTGGTGATCGAAAACGTCCTGCAGCTCTCCCGCCGTCGCC
AAGCCGAGCCGCAGTTGCTCGACCTCAAGTACTGGCTGCATCGCTTCGCCAGCGAGTTTCGCAGCTCGGCCCCCGCCGAC
CGCCAGCTCCACCTGGAGGCACGCGGCGGCGGACTGCAGACTCGAATGGATCCGCACCAGCTCAATCAGGTGCTGACCAA
CCTGGTGCAGAATGGCCTGCGCTACAGTGCTCAGAAGCACCGCCTTGGCCAGGTCTGGCTGCGCCTGTTCCGCGATGCGG
AAAGCGACCTGCCGGTACTGGAGATTCTCGACGACGGCCCCGGCGTGCCGCCCGAGCAGGAACCGCACATCTTCGAGCCC
TTCTACACTACCGAGAACAAAGGCACCGGCCTTGGCTTGTACATCTCGCGCGAGCTGTGCGAGAGCAACCAGGCTCGCCT
CGACTATAAACCTCGTGAAGGTGGCGGCAGTTGCTTTCGCATCACCTTCGCCCATCCGCGCAAACTGAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

68.199

98.491

0.672

Multiple sequence alignment