Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   AAHT65_RS20215 Genome accession   NZ_CP154443
Coordinates   4015224..4015847 (-) Length   207 a.a.
NCBI ID   WP_343310722.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain SW     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4010224..4020847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHT65_RS20185 (AAHT65_20180) rpsT 4010518..4010784 (+) 267 WP_003325396.1 30S ribosomal protein S20 -
  AAHT65_RS20190 (AAHT65_20185) holA 4010799..4011842 (-) 1044 WP_010789253.1 DNA polymerase III subunit delta -
  AAHT65_RS20195 (AAHT65_20190) - 4011882..4012031 (-) 150 WP_181279838.1 hypothetical protein -
  AAHT65_RS20200 (AAHT65_20195) - 4012072..4012206 (+) 135 WP_064505211.1 YqzM family protein -
  AAHT65_RS20205 (AAHT65_20200) comEC 4012247..4014580 (-) 2334 WP_343310721.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  AAHT65_RS20210 (AAHT65_20205) - 4014587..4015156 (-) 570 WP_003325392.1 ComE operon protein 2 -
  AAHT65_RS20215 (AAHT65_20210) comEA 4015224..4015847 (-) 624 WP_343310722.1 helix-hairpin-helix domain-containing protein Machinery gene
  AAHT65_RS20220 (AAHT65_20215) comER 4015931..4016749 (+) 819 WP_343310723.1 late competence protein ComER -
  AAHT65_RS20225 (AAHT65_20220) - 4016819..4017559 (-) 741 WP_003325388.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  AAHT65_RS20230 (AAHT65_20225) rsfS 4017556..4017912 (-) 357 WP_003325387.1 ribosome silencing factor -
  AAHT65_RS20235 (AAHT65_20230) yqeK 4017929..4018489 (-) 561 WP_088117608.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  AAHT65_RS20240 (AAHT65_20235) - 4018479..4019048 (-) 570 WP_003325385.1 nicotinate-nucleotide adenylyltransferase -
  AAHT65_RS20245 (AAHT65_20240) yhbY 4019060..4019350 (-) 291 WP_003325384.1 ribosome assembly RNA-binding protein YhbY -
  AAHT65_RS20250 (AAHT65_20245) aroE 4019344..4020186 (-) 843 WP_061572764.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 21962.75 Da        Isoelectric Point: 4.6181

>NTDB_id=993469 AAHT65_RS20215 WP_343310722.1 4015224..4015847(-) (comEA) [Bacillus atrophaeus strain SW]
MNWFYQHKKIILTAGAAVIFIVILINFPGAKKQEPVQQITASPDTAGTQVQQQESKGDADGEKIIIDIKGAVKHPGVYEL
KTGDRVSQAIEKAGGINSEADEKQVNLAELLQDGTVVYIPSEGEEPTQSAAATASVQNGAGKEALININTASLEELQAIS
GVGPSKAEAIIAYREENGKFQAVEDITNVSGIGEKSFEKIKSAITVK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=993469 AAHT65_RS20215 WP_343310722.1 4015224..4015847(-) (comEA) [Bacillus atrophaeus strain SW]
ATGAATTGGTTTTACCAGCACAAAAAAATAATCTTAACGGCAGGTGCTGCTGTCATCTTCATCGTCATCCTGATTAATTT
TCCCGGCGCAAAGAAGCAGGAGCCTGTTCAGCAAATCACAGCGTCGCCTGACACGGCGGGCACACAAGTTCAGCAGCAAG
AATCAAAAGGTGATGCTGACGGAGAAAAAATTATCATTGATATTAAAGGCGCTGTCAAACATCCGGGTGTTTACGAATTG
AAAACAGGAGACAGAGTTTCACAGGCTATCGAAAAAGCGGGAGGCATTAATTCGGAGGCTGATGAAAAACAAGTAAATTT
AGCTGAACTCCTGCAGGACGGCACTGTCGTGTACATTCCGAGTGAGGGGGAAGAGCCAACACAAAGCGCTGCCGCAACTG
CTTCTGTGCAAAACGGAGCAGGAAAAGAGGCTTTGATTAATATCAATACAGCGTCATTAGAGGAACTGCAGGCTATTTCC
GGGGTCGGTCCGTCAAAAGCAGAAGCTATAATAGCTTATCGTGAAGAAAACGGAAAATTTCAAGCCGTTGAAGACATTAC
AAATGTCTCGGGAATCGGCGAAAAATCATTTGAGAAAATAAAATCAGCCATCACAGTTAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(66-120)

Helix-hairpin-helix motif (HHH_3)

(143-205)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

65.7

100

0.657

Multiple sequence alignment