Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   AAHT66_RS20365 Genome accession   NZ_CP154441
Coordinates   3923241..3926945 (-) Length   1234 a.a.
NCBI ID   WP_333516283.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain XN303     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3918241..3931945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHT66_RS20350 (AAHT66_20350) hlpB 3918284..3918586 (-) 303 WP_289346869.1 HNH nuclease-like protein HlpB -
  AAHT66_RS20355 (AAHT66_20355) sbcC 3918603..3921995 (-) 3393 WP_333516282.1 exonuclease subunit SbcC -
  AAHT66_RS20360 (AAHT66_20360) sbcD 3921992..3923167 (-) 1176 WP_087993645.1 exonuclease subunit SbcD -
  AAHT66_RS20365 (AAHT66_20365) addA 3923241..3926945 (-) 3705 WP_333516283.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AAHT66_RS20370 (AAHT66_20370) addB 3926932..3930432 (-) 3501 WP_202328476.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AAHT66_RS20375 (AAHT66_20375) - 3930617..3931081 (+) 465 WP_253268993.1 ferritin family protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141316.88 Da        Isoelectric Point: 5.3209

>NTDB_id=993387 AAHT66_RS20365 WP_333516283.1 3923241..3926945(-) (addA) [Bacillus inaquosorum strain XN303]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRMADQAEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLHGAKEKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQGDFTELYKRVPAVSFKRAKAVKGDEFDPALLEEATDLRNSAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAENETGKREPSEAARFYQEQ
FREVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RTDILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETKTELLLIDNTEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENTKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRSFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPQMRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQPDWLLPEFDRYQAKTYLDFIGPALARHRDLENSAENGAAAHADISGHPARFAVRMIHSYD
LLDDDLEERMEEKSERLEAIRKGEPVPGSFAFDKKAREQLSWTYPHQEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKQGLTAAEKGTAMHTVMQHIPLTHVLTIEEAEQTVHRLYEKELLTEEQKAAIDIEEIVRFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETENGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=993387 AAHT66_RS20365 WP_333516283.1 3923241..3926945(-) (addA) [Bacillus inaquosorum strain XN303]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
CGTGGCAGCGGCGGCGGGCTCCGGAAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATCACCGCGGAAGAAAACC
CGATTGATGTAGACCGGCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCC
TTGGAAAAAGAGCTTGTAAAACGCCCCGGATCACTGCATATCAGGCGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACGCTTCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTAATCGATCTTGATCCGGGCTTTCGGATGGCTG
ATCAGGCAGAAGGCGAGCTGCTTGGGGATGAGGTGCTGGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAGAAGGCG
TTTTTTGAGCTTGTTGACCGCTACACGACAGACCGGCATGATCTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCCCGTTCCCACCCGAACCCGGAGGCATGGCTGGAGAGCTTTGTTCATTTGTATGATGTATCGGAAAAGAGCGCCATCG
AAGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGCGATGGTGCTTCACGGAGCGAAGGAAAAGCTCTTGCGCGCG
CTTGAGCTGACAAAAGCGCCGGGCGGCCCCGCGCCGCGCGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGGTGATTTCACTGAACTTTATAAACGGGTGCCCGCAGTCTCTTTTAAGCGCGCGAAAGCAGTAAAAG
GGGACGAGTTTGATCCTGCGCTCCTTGAAGAAGCGACGGATTTGAGAAATAGCGCAAAAAAACTGCTTGAAAAGCTTAAA
ACCGATTACTTCACGAGAAGTCCTGAACAGCATTTGAAAAGCTTGGCCGACATGAAGCCTGTCATTGAAACGCTTGTACA
GCTTGTCATCAGCTACGGAAAACGATTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTCTCTGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCAGAGAATGAAACAGGTAAACGCGAGCCGAGCGAGGCAGCAAGATTTTATCAGGAGCAG
TTTCGCGAGGTGCTCGTTGATGAATACCAGGATACCAACCTCGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGGCC
GGAAGAGACAGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCAATTTATCGATTCAGGCTTGCTGAGCCGCTTCTTT
TCCTCTCCAAATATAAACGATTTACAGAGAGCGGAGAAGGTACGGGACGGAAAATTGACTTGAATAAAAATTTCCGAAGC
CGGACTGATATTTTAGACAGCACGAACTTTTTATTTAAACAGCTAATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTATCCGGACAATGATGAGACGAAAACGGAGCTGCTGCTGATCGACAACACAG
AAGAGACGGATGCAGGTGAGGAAGCAGAAGAGCTGGAAACCGTGCAGTTCGAAGCGAAAGCGATCGCCAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTCCGTTCCATGCCGTGGGCGCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGTTATTTTGAAGCGGTCGAAGTCGCCGTCGCGCTTTCTGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTGCTGCGTTCCCCGATTGTCGGGGCGGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATACAAA
AGCGCCGTACTATGAAGCGATGAAAGACTATTTGGCGGCGGGTGACCGTAACGACGAGCTTTATCAAAAGCTGCAAACTT
TCTACGGACATCTGCAAAAATGGCGTTCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTCTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGCGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGTTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTGTTCGCGGCGGGTCTCGGCCGGAACTTCAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTTGGATTTGGCACGAAGTTTATCCATCCGCAAATGCGCATCAGCTACCCGACGCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGAGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTCGCATTAACGAGGGCGAAAGAAAAACTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAGAAACAGCTCGCAAAATGGCAGGCATCCGCGTCCCAACCTGACTGGCTTCTGCCCGA
ATTTGACCGCTACCAGGCGAAAACGTATTTAGATTTCATCGGGCCGGCTCTTGCCAGACACAGAGATTTGGAGAATTCAG
CTGAAAACGGTGCGGCAGCGCATGCTGATATATCAGGACACCCGGCTCGTTTTGCCGTTCGAATGATTCATTCCTATGAT
TTGCTTGATGATGATTTGGAAGAAAGAATGGAAGAAAAAAGCGAGCGCCTAGAAGCGATCCGCAAAGGTGAACCAGTTCC
CGGCTCGTTTGCATTTGATAAAAAAGCCCGCGAGCAGCTGAGTTGGACATACCCGCATCAAGAGGTCACACAAATTCGAA
CAAAGCAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCTCCCGTTAAACCGGCT
GACGGAAGCATTCTGTACAGGCGCCCCGCTTTTATGATGAAACAGGGCCTGACAGCGGCGGAGAAAGGGACAGCCATGCA
TACGGTGATGCAGCATATCCCGCTGACACATGTGCTGACGATAGAAGAAGCGGAGCAGACGGTTCACAGGCTTTATGAAA
AGGAGCTGCTCACTGAAGAACAAAAAGCCGCTATTGATATAGAAGAAATCGTGCGATTTTTCCATACGGAAATCGGCGGA
CAGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCATTTAGTTTAGCGCTTCCGGCAAAGGAAATCTATCCTGATGC
ACAGGAGGCGGATGAGCCGCTTTTGGTGCAGGGAATCATCGACTGTCTCTATGAAACTGAAAACGGCTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGCTTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAA
ACGCAAATTCAGCTGTACACGAAGGCGGTTGAGCAAATTGCGAAAACAAAGGTAAAAGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATACTGACATTATAG

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

95.948

100

0.959

Multiple sequence alignment