Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AAHT66_RS20370 Genome accession   NZ_CP154441
Coordinates   3926932..3930432 (-) Length   1166 a.a.
NCBI ID   WP_202328476.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain XN303     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3921932..3935432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHT66_RS20360 (AAHT66_20360) sbcD 3921992..3923167 (-) 1176 WP_087993645.1 exonuclease subunit SbcD -
  AAHT66_RS20365 (AAHT66_20365) addA 3923241..3926945 (-) 3705 WP_333516283.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AAHT66_RS20370 (AAHT66_20370) addB 3926932..3930432 (-) 3501 WP_202328476.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AAHT66_RS20375 (AAHT66_20375) - 3930617..3931081 (+) 465 WP_253268993.1 ferritin family protein -
  AAHT66_RS20380 (AAHT66_20380) - 3931270..3932997 (-) 1728 WP_253268992.1 SgrR family transcriptional regulator -
  AAHT66_RS20385 (AAHT66_20385) - 3933111..3934313 (+) 1203 WP_333516284.1 MDR family MFS transporter -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134571.46 Da        Isoelectric Point: 5.4734

>NTDB_id=993388 AAHT66_RS20370 WP_202328476.1 3926932..3930432(-) (addB) [Bacillus inaquosorum strain XN303]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLSDQYLHSEDYLTLLSEHIPLAEDIKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKAYYRLHQKAKELNLDITYKELNGTERHTQAPELAHLEAQYEARPSVPYGEKQEALTVMQAANR
RAELEGIVREIHALVREKGYRYKDVAILARQTEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSIDVLKGNWRY
EAVFRCVKTELLFPLNEPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQERAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMMEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTACTFVLGANDGVLPARPDENGVLSDDDREWLKTIGIELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVIKRLEELFPQHKERLLTNEPEQVSDEEQLKYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLDRGVSRQLYGERIQGSVSRMESFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKEQCELFSFDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCSFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=993388 AAHT66_RS20370 WP_202328476.1 3926932..3930432(-) (addB) [Bacillus inaquosorum strain XN303]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGAATGATACGTGCTCAAGTGTTCAGTTTTTCACGATTGGCCTGGCGCGTTCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTTACGAGCACAGGCGTACAGATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGCACAGGTAGAGCGCATGCTGACAGAGTTTAAGCGTTACT
GTCTGGAACCGGAAGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCCGAGTATCGCGGGGAACGTGTTTTATCTGAA
AAGCTTCATGACTTATCGATTCTGTATCAGCAGATGGAAAAAAGCCTTTCAGACCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGTCAGAGCATATCCCGCTTGCGGAAGATATAAAAGGCGCGCATATCTATGTGGACGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCAGAGCATATCACGTTTTCGCTGACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGAAAAGCCTATTACCGCCTTCATCAGAAGGCGAA
GGAACTGAATCTGGACATCACCTATAAAGAGCTAAACGGAACTGAGCGGCATACGCAGGCGCCGGAACTGGCGCATCTAG
AGGCGCAGTATGAAGCGCGTCCGTCTGTCCCGTACGGAGAAAAACAAGAGGCCTTAACTGTGATGCAGGCCGCAAATAGA
CGAGCTGAGCTGGAAGGCATCGTCCGGGAAATTCACGCGCTTGTCAGAGAGAAGGGCTATCGCTATAAAGATGTAGCGAT
TCTTGCGCGCCAGACGGAAGACTATAAGGATATGGTGAAGGAAGTTTTCGCTGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCTATGCTGAACCATCCGTTAATCGAATTTATCCGGTCGAGCATTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTTAACGAACCGAAGGCGAAGGTGAGAGAACAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGTATTAAAGGCGACCGCTGGACAAAGGGCGACCGTTTCCACTACAGACGTT
TTGTATCATTGGATGATGATTTTGCGCAGACCGATCAGGAAATCGAAATGGAAAACATGCTGAATGACACACGCGACTGG
ATTGTTCCGCCGCTTTTTCAGCTCCAGAAACGAATGAAAAAAGCTAAGACAGTTCAGGAGAGGGCGGAGGCGCTCTACCG
TTATTTAGAGGAGACAGATGTGCCGCTGAAGCTGGATCAGGAGAGACAGCGCGCTGAGGAAGACGGCAGAATCATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATCCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATT
TCTCTAGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTCACATTTTCTCTTATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTATCCAGAATGTATGGCACCGCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTC
TGCCGGCGCGCCCTGATGAAAACGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACCATAGGAATTGAACTTTCC
TCAGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGTTTTCCAGTCCGTCTGACCGGCTTTATGT
GTCGTATCCGATTGCTGATGCGGAAGGAAAAACGCTTTTGCCGTCGATTGTCATTAAGCGGCTGGAAGAGCTGTTTCCGC
AGCATAAGGAGCGCCTATTGACAAATGAACCTGAGCAGGTCAGCGATGAGGAACAGCTTAAGTATGTCGTCAATAAAAGC
GTGGCACAGTCCTTTACCGCAAGCCAGCTCAGATTATGGACTCGGGAATACGACATCAGCGACGTTTGGTGGAGCACGTA
CAATGTGCTAATGAGTGAGCCGGACAGGCTGCAATCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGATCGCGGCGTGTCGAGACAGCTCTATGGCGAACGCATTCAGGGCAGTGTATCGAGAATGGAAAGCTTTAACGCG
TGCCCATTCTCCCATTTTGCGTCACACGGGCTTCATCTGAAGGAACGGCAATTTTTCAAACTCGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACCGGCTGCGTGAGCAAAAGCTTGATTGGCGCGATTTAACGAAGG
AGCAGTGCGAGCTGTTTTCCTTTGATGCGGTAGAACGGCTGGCACCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTAAAGGAAAAGCTGCAAAAAATTGTGACCCGTGTGTCAGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTGCCGATCGGGCTTGAACTGGGCTTTGGGGGAAAAGGGCCCCTTCCGCCGCTGACCTTTACACTGAAAAACG
GCTGCACGATGGAGCTCGTCGGGCGAATAGACCGTGTCGATAAAGCGGAAAGCTCAAAAGGCCTGCTCCTCAGGATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGACTGGCGCTGCAAATGCTGACGTACCTTGA
CTTATCAATTACACATTCAGCCGACTGGCTCGGAATGAGAGCGACGCCGGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCCTG
CTCCTCGGCGATCAGGAAGCGGTTCGCCTCATGGATACGACCCTTCAAGAGGGACGGTCAAATATCATTAACGCCGGCTT
GAAGAAAGACGGCTCTCTCAGGTCGGATTCAGCGGCAGTCGGCGAAAAGGAATTTGAGCTGTTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAACAAATCACCGACGGACGTGTATCGATTGAGCCGTATAAAATGAAGAACAAGACGCCG
TGCACATACTGTTCGTTCAAATCAGTATGCCAATTTGATGAATCATTAGAAGAAAACGAGTATCGACCATTAAAGGCTGA
AAAGGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

97.17

100

0.972

Multiple sequence alignment