Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AAHT66_RS03060 Genome accession   NZ_CP154441
Coordinates   573953..575329 (-) Length   458 a.a.
NCBI ID   WP_087991673.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain XN303     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 568953..580329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHT66_RS03040 (AAHT66_03040) ispF 570468..570944 (-) 477 WP_087991677.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  AAHT66_RS03045 (AAHT66_03045) ispD 570937..571635 (-) 699 WP_333516844.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  AAHT66_RS03050 (AAHT66_03050) - 571650..572750 (-) 1101 WP_087991675.1 PIN/TRAM domain-containing protein -
  AAHT66_RS03055 (AAHT66_03055) disA 572867..573949 (-) 1083 WP_087991674.1 DNA integrity scanning diadenylate cyclase DisA -
  AAHT66_RS03060 (AAHT66_03060) radA 573953..575329 (-) 1377 WP_087991673.1 DNA repair protein RadA Machinery gene
  AAHT66_RS03065 (AAHT66_03065) clpC 575421..577853 (-) 2433 WP_087991672.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  AAHT66_RS03070 (AAHT66_03070) - 577850..578941 (-) 1092 WP_087991671.1 protein arginine kinase -
  AAHT66_RS03075 (AAHT66_03075) mcsA 578941..579498 (-) 558 WP_087991670.1 protein-arginine kinase activator protein McsA -
  AAHT66_RS03080 (AAHT66_03080) ctsR 579512..579976 (-) 465 WP_087991669.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49455.97 Da        Isoelectric Point: 8.2392

>NTDB_id=993325 AAHT66_RS03060 WP_087991673.1 573953..575329(-) (radA) [Bacillus inaquosorum strain XN303]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSDEPRVKTKLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSDSSNSVLYISGEESVKQTKLRADRLGINSPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=993325 AAHT66_RS03060 WP_087991673.1 573953..575329(-) (radA) [Bacillus inaquosorum strain XN303]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGTTATGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAGGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGATGAACCCCGCGTCAAAACCAAGCTTGGCGAATTT
AACAGGGTACTCGGCGGAGGTGTCGTTAAAGGCTCACTTGTTTTAATCGGCGGAGATCCCGGTATCGGAAAGTCAACGTT
ATTACTGCAAGTTTCTGCTCAATTATCAGACTCATCGAACAGTGTGCTGTACATTTCGGGAGAAGAATCGGTCAAACAAA
CGAAACTTAGAGCAGACCGTCTCGGCATTAATAGTCCGTCGCTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATCACATCGGC
TCCCGGCAGCGTGTCACAGGTCAGAGAATGTACGGCTGAGCTGATGAAAATCGCCAAAACAAAAGGCATTCCGATTTTTA
TCGTAGGACACGTGACAAAAGAAGGGTCTATTGCAGGGCCGAGATTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGTCACCATACCTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTTGGTTCTACAAACGAAATGGGTATTTT
TGAAATGCGCGAAGAGGGGCTCACCGAGGTTTTGAATCCTTCAGAAATTTTCTTGGAAGAACGCTCAGCGGGATCTGCAG
GCTCGAGTATAACTGCCTCTATGGAAGGCACAAGGCCGATTCTCGTTGAAATCCAGGCGCTTATCTCGCCGACAAGCTTT
GGCAATCCGCGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCGCTGTTAATGGCCGTGTTAGAAAAAAGAGTTGG
GCTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCCGGCGGCGTAAAGCTTGATGAACCGGCAATTGACCTTGCCA
TTGCGATCAGTATCGCGTCAAGCTTTAGAGATACACCTCCAAATCCTGCCGATTGTTTTATAGGTGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAGGAAGCTGCTAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAGGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

AAA domain (AAA_25)

(72-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

98.69

100

0.987
  radA Streptococcus pneumoniae Rx1

63.355

98.908

0.627
  radA Streptococcus pneumoniae D39

63.355

98.908

0.627
  radA Streptococcus pneumoniae R6

63.355

98.908

0.627
  radA Streptococcus pneumoniae TIGR4

63.355

98.908

0.627
  radA Streptococcus mitis NCTC 12261

63.355

98.908

0.627
  radA Streptococcus mitis SK321

63.135

98.908

0.624

Multiple sequence alignment