Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AAIA70_RS14745 Genome accession   NZ_CP154264
Coordinates   3187217..3188233 (-) Length   338 a.a.
NCBI ID   WP_343291032.1    Uniprot ID   -
Organism   Vibrio harveyi strain 45T2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3182217..3193233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAIA70_RS14720 (AAIA70_14720) aroK 3183073..3183591 (-) 519 WP_005451507.1 shikimate kinase AroK -
  AAIA70_RS14725 (AAIA70_14725) pilQ 3183781..3185532 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ family protein Machinery gene
  AAIA70_RS14730 (AAIA70_14730) pilP 3185567..3186082 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  AAIA70_RS14735 (AAIA70_14735) pilO 3186072..3186665 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAIA70_RS14740 (AAIA70_14740) pilN 3186658..3187233 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  AAIA70_RS14745 (AAIA70_14745) pilM 3187217..3188233 (-) 1017 WP_343291032.1 type IV pilus assembly protein PilM Machinery gene
  AAIA70_RS14750 (AAIA70_14750) - 3188412..3190982 (+) 2571 WP_050937527.1 PBP1A family penicillin-binding protein -
  AAIA70_RS14755 (AAIA70_14755) oxyR 3191049..3191957 (-) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  AAIA70_RS14760 (AAIA70_14760) - 3192132..3192860 (+) 729 WP_005451494.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38087.46 Da        Isoelectric Point: 6.4206

>NTDB_id=992806 AAIA70_RS14745 WP_343291032.1 3187217..3188233(-) (pilM) [Vibrio harveyi strain 45T2]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVSISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDSYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHMWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=992806 AAIA70_RS14745 WP_343291032.1 3187217..3188233(-) (pilM) [Vibrio harveyi strain 45T2]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCTCAATTTCTGTTCCTGATAATGCC
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACA
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGCGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATGTGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGAACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAAGTTGCTACTGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGTAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGCTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.237

100

0.902
  pilM Vibrio cholerae strain A1552

48.538

100

0.491

Multiple sequence alignment