Detailed information NTG0992625

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHU14_RS21895
• Genome accession: NZ_CP154196
• Coordinates: 4491585..4492385 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain KP_NORM_BLD_1339
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4486585..4497385

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHU14_RS21860 (AAHU14_21825)	gspH	4486738..4487253 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHU14_RS21865 (AAHU14_21830)	gspI	4487250..4487615 (+)	366	WP_049139126.1	type II secretion system minor pseudopilin GspI	-
AAHU14_RS21870 (AAHU14_21835)	gspJ	4487612..4488208 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
AAHU14_RS21875 (AAHU14_21840)	gspK	4488205..4489185 (+)	981	WP_086892928.1	type II secretion system minor pseudopilin GspK	-
AAHU14_RS21880 (AAHU14_21845)	gspL	4489178..4490374 (+)	1197	WP_077139048.1	type II secretion system protein GspL	-
AAHU14_RS21885 (AAHU14_21850)	-	4490367..4490852 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHU14_RS21890 (AAHU14_21855)	-	4490842..4491588 (+)	747	WP_012968922.1	type II secretion system protein N	-
AAHU14_RS21895 (AAHU14_21860)	pilD	4491585..4492385 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAHU14_RS21900 (AAHU14_21865)	hrpB	4492432..4494861 (-)	2430	WP_032740432.1	ATP-dependent helicase HrpB	-
AAHU14_RS21905 (AAHU14_21870)	thpR	4494934..4495470 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHU14_RS21910 (AAHU14_21875)	sfsA	4495470..4496186 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHU14_RS21915 (AAHU14_21880)	dksA	4496349..4496804 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=992625 AAHU14_RS21895 WP_008807384.1 4491585..4492385(+) (pilD) [Klebsiella variicola subsp. variicola strain KP_NORM_BLD_1339]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=992625 AAHU14_RS21895 WP_008807384.1 4491585..4492385(+) (pilD) [Klebsiella variicola subsp. variicola strain KP_NORM_BLD_1339]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCACTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425