Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACKTSO_RS02490 Genome accession   NZ_AP035889
Coordinates   514607..515167 (-) Length   186 a.a.
NCBI ID   WP_039081188.1    Uniprot ID   -
Organism   Gallibacterium anatis strain SMUHGa01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 509607..520167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKTSO_RS02470 (SMUG_04660) - 510312..510932 (-) 621 WP_285093197.1 tetratricopeptide repeat protein -
  ACKTSO_RS02475 (SMUG_04670) hisS 510944..512227 (-) 1284 WP_039154755.1 histidine--tRNA ligase -
  ACKTSO_RS02480 (SMUG_04680) ispG 512249..513364 (-) 1116 WP_039140601.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACKTSO_RS02485 (SMUG_04690) - 513389..514462 (-) 1074 WP_039154753.1 RodZ domain-containing protein -
  ACKTSO_RS02490 (SMUG_04700) pilF 514607..515167 (-) 561 WP_039081188.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACKTSO_RS02495 (SMUG_04710) - 515188..516306 (-) 1119 WP_039083483.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACKTSO_RS02500 (SMUG_04720) folD 516624..517487 (-) 864 WP_407902808.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD -
  ACKTSO_RS02515 (SMUG_04730) - 518028..518381 (+) 354 WP_407902809.1 transposase -
  ACKTSO_RS02520 - 518384..519214 (+) 831 Protein_475 IS3 family transposase -
  ACKTSO_RS02525 (SMUG_04760) - 519227..519463 (-) 237 WP_052115767.1 hypothetical protein -
  ACKTSO_RS02530 (SMUG_04770) - 519460..519639 (-) 180 WP_013745256.1 hypothetical protein -

Sequence

Protein


Download         Length: 186 a.a.        Molecular weight: 20981.90 Da        Isoelectric Point: 9.1368

>NTDB_id=99262 ACKTSO_RS02490 WP_039081188.1 514607..515167(-) (pilF) [Gallibacterium anatis strain SMUHGa01]
MNKTMIKLKFFILLSSLFTLFACSHSVSNKPLSANAAAAKTRVELGLAYLSQGNPALAKANLDKALQHTPNDYLPYLGLA
YFYQHTQQLKLAGEYYQKALSLSPENGDVLNNYATYLCSQRQFDTAFNFFEQALNSKAYYHFADTYENIAICAVLAGDQN
KTEQALTQLEKYSVDKAKNLRQRLHK

Nucleotide


Download         Length: 561 bp        

>NTDB_id=99262 ACKTSO_RS02490 WP_039081188.1 514607..515167(-) (pilF) [Gallibacterium anatis strain SMUHGa01]
ATGAACAAAACAATGATTAAACTGAAATTTTTTATTCTGCTTTCCTCACTGTTTACGCTTTTTGCCTGTAGCCATTCAGT
TAGCAATAAACCACTTTCCGCTAATGCTGCTGCGGCAAAAACGCGTGTTGAATTAGGATTGGCTTATTTGTCGCAAGGCA
ATCCGGCATTGGCAAAAGCCAATTTAGATAAAGCGTTGCAACATACACCGAATGATTATTTGCCTTATCTAGGTTTGGCT
TATTTTTATCAACATACCCAACAACTCAAACTTGCCGGCGAATATTATCAAAAAGCGCTCTCTCTTTCGCCCGAGAATGG
CGATGTATTGAATAATTATGCAACTTACCTCTGCTCTCAACGCCAATTTGATACGGCATTTAACTTTTTTGAACAAGCAT
TAAACAGCAAAGCCTATTATCATTTTGCCGATACTTATGAAAACATTGCGATTTGCGCTGTTTTAGCCGGTGATCAGAAC
AAAACTGAACAAGCTTTAACCCAACTGGAGAAATATTCTGTCGATAAAGCGAAAAATTTGCGGCAACGTTTGCATAAATA
A

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_8)

(40-68)

Tetratricopeptide repeat (TPR_17)

(96-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

44.809

98.387

0.441
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.988

89.247

0.419

Multiple sequence alignment