Detailed information NTG0992554

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHU30_RS22230
• Genome accession: NZ_CP154159
• Coordinates: 4552765..4553565 (+)
• Length: 266 a.a.
• NCBI ID: WP_023322076.1
• Uniprot ID: -
• Organism: Klebsiella variicola subsp. variicola strain KP_NORM_BLD_13886
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4547765..4558565

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHU30_RS22195 (AAHU30_22180)	gspH	4547918..4548433 (+)	516	WP_119867130.1	type II secretion system minor pseudopilin GspH	-
AAHU30_RS22200 (AAHU30_22185)	gspI	4548430..4548795 (+)	366	WP_119867131.1	type II secretion system minor pseudopilin GspI	-
AAHU30_RS22205 (AAHU30_22190)	gspJ	4548792..4549388 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
AAHU30_RS22210 (AAHU30_22195)	gspK	4549385..4550365 (+)	981	WP_012542824.1	type II secretion system minor pseudopilin GspK	-
AAHU30_RS22215 (AAHU30_22200)	gspL	4550358..4551554 (+)	1197	WP_119867132.1	type II secretion system protein GspL	-
AAHU30_RS22220 (AAHU30_22205)	-	4551547..4552032 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHU30_RS22225 (AAHU30_22210)	-	4552022..4552768 (+)	747	WP_044613513.1	type II secretion system protein N	-
AAHU30_RS22230 (AAHU30_22215)	pilD	4552765..4553565 (+)	801	WP_023322076.1	A24 family peptidase	Machinery gene
AAHU30_RS22235 (AAHU30_22220)	hrpB	4553612..4556041 (-)	2430	WP_046620841.1	ATP-dependent helicase HrpB	-
AAHU30_RS22240 (AAHU30_22225)	thpR	4556114..4556650 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHU30_RS22245 (AAHU30_22230)	sfsA	4556650..4557366 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHU30_RS22250 (AAHU30_22235)	dksA	4557529..4557984 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29445.19 Da
• Isoelectric Point: 8.7151

>NTDB_id=992554 AAHU30_RS22230 WP_023322076.1 4552765..4553565(+) (pilD) [Klebsiella variicola subsp. variicola strain KP_NORM_BLD_13886]
MTILTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=992554 AAHU30_RS22230 WP_023322076.1 4552765..4553565(+) (pilD) [Klebsiella variicola subsp. variicola strain KP_NORM_BLD_13886]
ATGACGATATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425