Detailed information NTG0991300

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAH428_RS20620
• Genome accession: NZ_CP153634
• Coordinates: 4185803..4186603 (+)
• Length: 266 a.a.
• NCBI ID: WP_021441214.1
• Uniprot ID: A0A4V0HF22
• Organism: Klebsiella pneumoniae strain Kpn1537
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4180803..4191603

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAH428_RS20585 (AAH428_20555)	gspH	4180959..4181468 (+)	510	WP_021441218.1	type II secretion system minor pseudopilin GspH	-
AAH428_RS20590 (AAH428_20560)	gspI	4181471..4181836 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
AAH428_RS20595 (AAH428_20565)	gspJ	4181833..4182429 (+)	597	WP_032428541.1	type II secretion system minor pseudopilin GspJ	-
AAH428_RS20600 (AAH428_20570)	gspK	4182426..4183412 (+)	987	WP_004183041.1	type II secretion system minor pseudopilin GspK	-
AAH428_RS20605 (AAH428_20575)	gspL	4183399..4184592 (+)	1194	WP_064148303.1	type II secretion system protein GspL	-
AAH428_RS20610 (AAH428_20580)	-	4184585..4185070 (+)	486	WP_004151943.1	type II secretion system protein M	-
AAH428_RS20615 (AAH428_20585)	-	4185060..4185806 (+)	747	WP_064148304.1	type II secretion system protein N	-
AAH428_RS20620 (AAH428_20590)	pilD	4185803..4186603 (+)	801	WP_021441214.1	A24 family peptidase	Machinery gene
AAH428_RS20625 (AAH428_20595)	hrpB	4186651..4189080 (-)	2430	WP_023282188.1	ATP-dependent helicase HrpB	-
AAH428_RS20630 (AAH428_20600)	thpR	4189153..4189689 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
AAH428_RS20635 (AAH428_20605)	sfsA	4189689..4190405 (+)	717	WP_064148305.1	DNA/RNA nuclease SfsA	-
AAH428_RS20640 (AAH428_20610)	dksA	4190568..4191023 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=991300 AAH428_RS20620 WP_021441214.1 4185803..4186603(+) (pilD) [Klebsiella pneumoniae strain Kpn1537]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
SRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=991300 AAH428_RS20620 WP_021441214.1 4185803..4186603(+) (pilD) [Klebsiella pneumoniae strain Kpn1537]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
AGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
TCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

3D structure

• AlphaFold DB: A0A4V0HF22

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425