Detailed information NTG0991252

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAH410_RS21780
• Genome accession: NZ_CP153630
• Coordinates: 4443232..4444032 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain Kpn347
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4438232..4449032

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAH410_RS21745 (AAH410_21730)	gspH	4438385..4438900 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAH410_RS21750 (AAH410_21735)	gspI	4438897..4439262 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAH410_RS21755 (AAH410_21740)	gspJ	4439259..4439855 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
AAH410_RS21760 (AAH410_21745)	gspK	4439852..4440832 (+)	981	WP_117106667.1	type II secretion system minor pseudopilin GspK	-
AAH410_RS21765 (AAH410_21750)	gspL	4440825..4442021 (+)	1197	WP_373853650.1	type II secretion system protein GspL	-
AAH410_RS21770 (AAH410_21755)	-	4442014..4442499 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAH410_RS21775 (AAH410_21760)	-	4442489..4443235 (+)	747	WP_117106665.1	type II secretion system protein N	-
AAH410_RS21780 (AAH410_21765)	pilD	4443232..4444032 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAH410_RS21785 (AAH410_21770)	hrpB	4444079..4446508 (-)	2430	WP_117106664.1	ATP-dependent helicase HrpB	-
AAH410_RS21790 (AAH410_21775)	thpR	4446581..4447117 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAH410_RS21795 (AAH410_21780)	sfsA	4447117..4447833 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAH410_RS21800 (AAH410_21785)	dksA	4447996..4448451 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=991252 AAH410_RS21780 WP_008807384.1 4443232..4444032(+) (pilD) [Klebsiella variicola subsp. variicola strain Kpn347]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=991252 AAH410_RS21780 WP_008807384.1 4443232..4444032(+) (pilD) [Klebsiella variicola subsp. variicola strain Kpn347]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425