Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   VV34_RS07620 Genome accession   NZ_LN649259
Coordinates   1435602..1437701 (-) Length   699 a.a.
NCBI ID   WP_009967104.1    Uniprot ID   O31673
Organism   Bacillus subtilis strain BS49     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 1430602..1442701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VV34_RS07590 (BS49_15160) spo0E 1430658..1430915 (+) 258 WP_003218598.1 aspartyl-phosphate phosphatase Spo0E -
  VV34_RS07595 (BS49_15170) - 1431001..1431432 (+) 432 WP_003245685.1 hypothetical protein -
  VV34_RS07600 (BS49_15180) kinD 1431460..1432980 (-) 1521 WP_010886497.1 sporulation kinase KinD -
  VV34_RS07605 (BS49_15190) mhqR 1433173..1433610 (+) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  VV34_RS07610 (BS49_15200) motB 1433650..1434435 (-) 786 WP_003232473.1 flagellar motor protein MotB -
  VV34_RS07615 (BS49_15210) motA 1434407..1435219 (-) 813 WP_003244739.1 flagellar motor stator protein MotA -
  VV34_RS07620 (BS49_15220) clpE 1435602..1437701 (-) 2100 WP_009967104.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  VV34_RS07625 (BS49_15230) - 1438066..1439109 (+) 1044 WP_003244712.1 hypothetical protein -
  VV34_RS07630 (BS49_15240) queC 1439422..1440081 (+) 660 WP_003245417.1 7-cyano-7-deazaguanine synthase QueC -
  VV34_RS07635 (BS49_15250) queD 1440074..1440523 (+) 450 WP_003232462.1 6-carboxytetrahydropterin synthase QueD -
  VV34_RS07640 (BS49_15260) queE 1440516..1441247 (+) 732 WP_003232460.1 7-carboxy-7-deazaguanine synthase QueE -
  VV34_RS07645 (BS49_15270) queF 1441265..1441762 (+) 498 WP_003218613.1 preQ(1) synthase -
  VV34_RS07650 (BS49_15280) ykvN 1442321..1442677 (-) 357 WP_003245808.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77905.96 Da        Isoelectric Point: 5.0886

>NTDB_id=991071 VV34_RS07620 WP_009967104.1 1435602..1437701(-) (clpE) [Bacillus subtilis strain BS49]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEQSAAKQSEKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=991071 VV34_RS07620 WP_009967104.1 1435602..1437701(-) (clpE) [Bacillus subtilis strain BS49]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAACAGAGCGCAGCAAAACAAAGCGAAAAAAAAGGGTTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCAAAAGCCGGTCTCATTGATCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGAAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTAGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGTGATGTTCCAAACAAACTAAAAAACAAAGAGCTATACTTGCTTGATGTTGCATCTCTTGTTGCA
AACACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCATGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAACAAAAGCCCTGGAAGAAGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTGATCGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAAGCGGTAAGACG
AAGCCGCGCCGGATTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAGGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGATGGCCGTCTGACAGACAGCCAAGGCAGAACTGTAAGCTTCAAAGACACTGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTAATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCGGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCAAAATAA

Domains

Predicted by InterProScan.

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

AAA lid domain (AAA_lid_9)

(253-352)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O31673

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.662

100

0.602
  clpC Lactococcus lactis subsp. cremoris KW2

56.284

100

0.589
  clpE Streptococcus pneumoniae TIGR4

54.924

100

0.567
  clpE Streptococcus pneumoniae Rx1

54.924

100

0.567
  clpE Streptococcus pneumoniae D39

54.924

100

0.567
  clpE Streptococcus pneumoniae R6

54.924

100

0.567
  clpC Bacillus subtilis subsp. subtilis str. 168

54.016

90.844

0.491
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.847

89.7

0.429
  clpC Streptococcus thermophilus LMD-9

45.427

93.848

0.426
  clpC Streptococcus pneumoniae D39

46.25

91.559

0.423
  clpC Streptococcus pneumoniae Rx1

46.25

91.559

0.423
  clpC Streptococcus thermophilus LMG 18311

44.97

93.848

0.422
  clpC Streptococcus pneumoniae TIGR4

46.85

88.555

0.415
  clpC Streptococcus mutans UA159

46.216

88.841

0.411
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

42.079

86.695

0.365

Multiple sequence alignment