Detailed information NTG0990827

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHY02_RS22300
• Genome accession: NZ_CP153409
• Coordinates: 4544142..4544942 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain T7-165
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4539142..4549942

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHY02_RS22265 (AAHY02_22240)	gspH	4539295..4539810 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHY02_RS22270 (AAHY02_22245)	gspI	4539807..4540172 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHY02_RS22275 (AAHY02_22250)	gspJ	4540169..4540765 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
AAHY02_RS22280 (AAHY02_22255)	gspK	4540762..4541742 (+)	981	WP_012542824.1	type II secretion system minor pseudopilin GspK	-
AAHY02_RS22285 (AAHY02_22260)	gspL	4541735..4542931 (+)	1197	WP_039102856.1	type II secretion system protein GspL	-
AAHY02_RS22290 (AAHY02_22265)	-	4542924..4543409 (+)	486	WP_050887330.1	type II secretion system protein M	-
AAHY02_RS22295 (AAHY02_22270)	-	4543399..4544145 (+)	747	WP_012968922.1	type II secretion system protein N	-
AAHY02_RS22300 (AAHY02_22275)	pilD	4544142..4544942 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAHY02_RS22305 (AAHY02_22280)	hrpB	4544989..4547418 (-)	2430	WP_373879156.1	ATP-dependent helicase HrpB	-
AAHY02_RS22310 (AAHY02_22285)	thpR	4547491..4548027 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHY02_RS22315 (AAHY02_22290)	sfsA	4548027..4548743 (+)	717	WP_064166833.1	DNA/RNA nuclease SfsA	-
AAHY02_RS22320 (AAHY02_22295)	dksA	4548906..4549361 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=990827 AAHY02_RS22300 WP_008807384.1 4544142..4544942(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-165]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990827 AAHY02_RS22300 WP_008807384.1 4544142..4544942(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-165]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425