Detailed information NTG0990804

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHY28_RS23175
• Genome accession: NZ_CP153399
• Coordinates: 4659316..4660116 (+)
• Length: 266 a.a.
• NCBI ID: WP_032731627.1
• Uniprot ID: -
• Organism: Klebsiella variicola subsp. variicola strain T7-311
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4654316..4665116

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHY28_RS23140 (AAHY28_23120)	gspH	4654469..4654984 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHY28_RS23145 (AAHY28_23125)	gspI	4654981..4655346 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHY28_RS23150 (AAHY28_23130)	gspJ	4655343..4655939 (+)	597	WP_032731630.1	type II secretion system minor pseudopilin GspJ	-
AAHY28_RS23155 (AAHY28_23135)	gspK	4655936..4656916 (+)	981	WP_012542824.1	type II secretion system minor pseudopilin GspK	-
AAHY28_RS23160 (AAHY28_23140)	gspL	4656909..4658105 (+)	1197	WP_032731629.1	type II secretion system protein GspL	-
AAHY28_RS23165 (AAHY28_23145)	-	4658098..4658583 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHY28_RS23170 (AAHY28_23150)	-	4658573..4659319 (+)	747	WP_032731628.1	type II secretion system protein N	-
AAHY28_RS23175 (AAHY28_23155)	pilD	4659316..4660116 (+)	801	WP_032731627.1	A24 family peptidase	Machinery gene
AAHY28_RS23180 (AAHY28_23160)	hrpB	4660163..4662592 (-)	2430	WP_285202203.1	ATP-dependent helicase HrpB	-
AAHY28_RS23185 (AAHY28_23165)	thpR	4662665..4663201 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHY28_RS23190 (AAHY28_23170)	sfsA	4663201..4663917 (+)	717	WP_022066362.1	DNA/RNA nuclease SfsA	-
AAHY28_RS23195 (AAHY28_23175)	dksA	4664080..4664535 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29463.17 Da
• Isoelectric Point: 8.7151

>NTDB_id=990804 AAHY28_RS23175 WP_032731627.1 4659316..4660116(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-311]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
SRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990804 AAHY28_RS23175 WP_032731627.1 4659316..4660116(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-311]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACACTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
AGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425