Detailed information NTG0990732

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHV86_RS22115
• Genome accession: NZ_CP153315
• Coordinates: 4530099..4530899 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain KP_NORM_URN_57447
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4525099..4535899

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHV86_RS22080 (AAHV86_22035)	gspH	4525252..4525767 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHV86_RS22085 (AAHV86_22040)	gspI	4525764..4526129 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHV86_RS22090 (AAHV86_22045)	gspJ	4526126..4526722 (+)	597	WP_023297079.1	type II secretion system minor pseudopilin GspJ	-
AAHV86_RS22095 (AAHV86_22050)	gspK	4526719..4527699 (+)	981	WP_374736101.1	type II secretion system minor pseudopilin GspK	-
AAHV86_RS22100 (AAHV86_22055)	gspL	4527692..4528888 (+)	1197	WP_374736102.1	type II secretion system protein GspL	-
AAHV86_RS22105 (AAHV86_22060)	-	4528881..4529366 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHV86_RS22110 (AAHV86_22065)	-	4529356..4530102 (+)	747	WP_172749222.1	type II secretion system protein N	-
AAHV86_RS22115 (AAHV86_22070)	pilD	4530099..4530899 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAHV86_RS22120 (AAHV86_22075)	hrpB	4530946..4533375 (-)	2430	WP_032740432.1	ATP-dependent helicase HrpB	-
AAHV86_RS22125 (AAHV86_22080)	thpR	4533448..4533984 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHV86_RS22130 (AAHV86_22085)	sfsA	4533984..4534700 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHV86_RS22135 (AAHV86_22090)	dksA	4534863..4535318 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=990732 AAHV86_RS22115 WP_008807384.1 4530099..4530899(+) (pilD) [Klebsiella variicola subsp. variicola strain KP_NORM_URN_57447]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990732 AAHV86_RS22115 WP_008807384.1 4530099..4530899(+) (pilD) [Klebsiella variicola subsp. variicola strain KP_NORM_URN_57447]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425