Detailed information NTG0990686

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHV90_RS21995
• Genome accession: NZ_CP153308
• Coordinates: 4520294..4521094 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain KP_NORM_URN_58012
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4515294..4526094

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHV90_RS21960 (AAHV90_21930)	gspH	4515447..4515962 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHV90_RS21965 (AAHV90_21935)	gspI	4515959..4516324 (+)	366	WP_049139126.1	type II secretion system minor pseudopilin GspI	-
AAHV90_RS21970 (AAHV90_21940)	gspJ	4516321..4516917 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
AAHV90_RS21975 (AAHV90_21945)	gspK	4516914..4517894 (+)	981	WP_012542824.1	type II secretion system minor pseudopilin GspK	-
AAHV90_RS21980 (AAHV90_21950)	gspL	4517887..4519083 (+)	1197	WP_077139048.1	type II secretion system protein GspL	-
AAHV90_RS21985 (AAHV90_21955)	-	4519076..4519561 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHV90_RS21990 (AAHV90_21960)	-	4519551..4520297 (+)	747	WP_117122463.1	type II secretion system protein N	-
AAHV90_RS21995 (AAHV90_21965)	pilD	4520294..4521094 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAHV90_RS22000 (AAHV90_21970)	hrpB	4521141..4523570 (-)	2430	WP_032740432.1	ATP-dependent helicase HrpB	-
AAHV90_RS22005 (AAHV90_21975)	thpR	4523643..4524179 (+)	537	WP_117122462.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHV90_RS22010 (AAHV90_21980)	sfsA	4524179..4524895 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHV90_RS22015 (AAHV90_21985)	dksA	4525058..4525513 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=990686 AAHV90_RS21995 WP_008807384.1 4520294..4521094(+) (pilD) [Klebsiella variicola subsp. variicola strain KP_NORM_URN_58012]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990686 AAHV90_RS21995 WP_008807384.1 4520294..4521094(+) (pilD) [Klebsiella variicola subsp. variicola strain KP_NORM_URN_58012]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCACTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425