Detailed information NTG0990662

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHY01_RS22060
• Genome accession: NZ_CP153281
• Coordinates: 4508490..4509290 (+)
• Length: 266 a.a.
• NCBI ID: WP_023322076.1
• Uniprot ID: -
• Organism: Klebsiella variicola subsp. variicola strain T7-160
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4503490..4514290

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHY01_RS22025 (AAHY01_21990)	gspH	4503643..4504158 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHY01_RS22030 (AAHY01_21995)	gspI	4504155..4504520 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHY01_RS22035 (AAHY01_22000)	gspJ	4504517..4505113 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
AAHY01_RS22040 (AAHY01_22005)	gspK	4505110..4506090 (+)	981	WP_012542824.1	type II secretion system minor pseudopilin GspK	-
AAHY01_RS22045 (AAHY01_22010)	gspL	4506083..4507279 (+)	1197	WP_117112559.1	type II secretion system protein GspL	-
AAHY01_RS22050 (AAHY01_22015)	-	4507272..4507757 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHY01_RS22055 (AAHY01_22020)	-	4507747..4508493 (+)	747	WP_044613513.1	type II secretion system protein N	-
AAHY01_RS22060 (AAHY01_22025)	pilD	4508490..4509290 (+)	801	WP_023322076.1	A24 family peptidase	Machinery gene
AAHY01_RS22065 (AAHY01_22030)	hrpB	4509337..4511766 (-)	2430	WP_110199892.1	ATP-dependent helicase HrpB	-
AAHY01_RS22070 (AAHY01_22035)	thpR	4511839..4512375 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHY01_RS22075 (AAHY01_22040)	sfsA	4512375..4513091 (+)	717	WP_022066362.1	DNA/RNA nuclease SfsA	-
AAHY01_RS22080 (AAHY01_22045)	dksA	4513254..4513709 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29445.19 Da
• Isoelectric Point: 8.7151

>NTDB_id=990662 AAHY01_RS22060 WP_023322076.1 4508490..4509290(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-160]
MTILTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990662 AAHY01_RS22060 WP_023322076.1 4508490..4509290(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-160]
ATGACGATATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAAGTGTACATTGCCCTACCGGACGCGGTCGCCGGGGCAATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425