Detailed information NTG0990615

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHX92_RS22760
• Genome accession: NZ_CP153239
• Coordinates: 4636708..4637508 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain T7-105
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4631708..4642508

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHX92_RS22725 (AAHX92_22685)	gspH	4631861..4632376 (+)	516	WP_072123494.1	type II secretion system minor pseudopilin GspH	-
AAHX92_RS22730 (AAHX92_22690)	gspI	4632373..4632738 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHX92_RS22735 (AAHX92_22695)	gspJ	4632735..4633331 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
AAHX92_RS22740 (AAHX92_22700)	gspK	4633328..4634308 (+)	981	WP_012542824.1	type II secretion system minor pseudopilin GspK	-
AAHX92_RS22745 (AAHX92_22705)	gspL	4634301..4635497 (+)	1197	WP_110409017.1	type II secretion system protein GspL	-
AAHX92_RS22750 (AAHX92_22710)	-	4635490..4635975 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHX92_RS22755 (AAHX92_22715)	-	4635965..4636711 (+)	747	WP_243814498.1	type II secretion system protein N	-
AAHX92_RS22760 (AAHX92_22720)	pilD	4636708..4637508 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAHX92_RS22765 (AAHX92_22725)	hrpB	4637555..4639984 (-)	2430	WP_232684282.1	ATP-dependent helicase HrpB	-
AAHX92_RS22770 (AAHX92_22730)	thpR	4640057..4640593 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHX92_RS22775 (AAHX92_22735)	sfsA	4640593..4641309 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHX92_RS22780 (AAHX92_22740)	dksA	4641472..4641927 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=990615 AAHX92_RS22760 WP_008807384.1 4636708..4637508(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-105]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990615 AAHX92_RS22760 WP_008807384.1 4636708..4637508(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-105]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGAGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425