Detailed information NTG0990592

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHX96_RS22125
• Genome accession: NZ_CP153222
• Coordinates: 4464060..4464860 (+)
• Length: 266 a.a.
• NCBI ID: WP_023322076.1
• Uniprot ID: -
• Organism: Klebsiella variicola subsp. variicola strain T7-133
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4459060..4469860

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHX96_RS22090 (AAHX96_22040)	gspH	4459213..4459728 (+)	516	WP_049139125.1	type II secretion system minor pseudopilin GspH	-
AAHX96_RS22095 (AAHX96_22045)	gspI	4459725..4460090 (+)	366	WP_049139126.1	type II secretion system minor pseudopilin GspI	-
AAHX96_RS22100 (AAHX96_22050)	gspJ	4460087..4460683 (+)	597	WP_023297079.1	type II secretion system minor pseudopilin GspJ	-
AAHX96_RS22105 (AAHX96_22055)	gspK	4460680..4461660 (+)	981	WP_077258660.1	type II secretion system minor pseudopilin GspK	-
AAHX96_RS22110 (AAHX96_22060)	gspL	4461653..4462849 (+)	1197	WP_039102856.1	type II secretion system protein GspL	-
AAHX96_RS22115 (AAHX96_22065)	-	4462842..4463327 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHX96_RS22120 (AAHX96_22070)	-	4463317..4464063 (+)	747	WP_023322077.1	type II secretion system protein N	-
AAHX96_RS22125 (AAHX96_22075)	pilD	4464060..4464860 (+)	801	WP_023322076.1	A24 family peptidase	Machinery gene
AAHX96_RS22130 (AAHX96_22080)	hrpB	4464907..4467336 (-)	2430	WP_049139127.1	ATP-dependent helicase HrpB	-
AAHX96_RS22135 (AAHX96_22085)	thpR	4467409..4467945 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHX96_RS22140 (AAHX96_22090)	sfsA	4467945..4468661 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHX96_RS22145 (AAHX96_22095)	dksA	4468824..4469279 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29445.19 Da
• Isoelectric Point: 8.7151

>NTDB_id=990592 AAHX96_RS22125 WP_023322076.1 4464060..4464860(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-133]
MTILTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990592 AAHX96_RS22125 WP_023322076.1 4464060..4464860(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-133]
ATGACGATATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCGTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425