Detailed information NTG0990546

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHX83_RS21735
• Genome accession: NZ_CP153197
• Coordinates: 4484049..4484849 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain T7-050
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4479049..4489849

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHX83_RS21700 (AAHX83_21695)	gspH	4479202..4479717 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHX83_RS21705 (AAHX83_21700)	gspI	4479714..4480079 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHX83_RS21710 (AAHX83_21705)	gspJ	4480076..4480672 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
AAHX83_RS21715 (AAHX83_21710)	gspK	4480669..4481649 (+)	981	WP_008807380.1	type II secretion system minor pseudopilin GspK	-
AAHX83_RS21720 (AAHX83_21715)	gspL	4481642..4482838 (+)	1197	WP_008807381.1	type II secretion system protein GspL	-
AAHX83_RS21725 (AAHX83_21720)	-	4482831..4483316 (+)	486	WP_008807382.1	type II secretion system protein M	-
AAHX83_RS21730 (AAHX83_21725)	-	4483306..4484052 (+)	747	WP_008807383.1	type II secretion system protein N	-
AAHX83_RS21735 (AAHX83_21730)	pilD	4484049..4484849 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAHX83_RS21740 (AAHX83_21735)	hrpB	4484896..4487325 (-)	2430	WP_048331184.1	ATP-dependent helicase HrpB	-
AAHX83_RS21745 (AAHX83_21740)	thpR	4487398..4487934 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHX83_RS21750 (AAHX83_21745)	sfsA	4487934..4488650 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHX83_RS21755 (AAHX83_21750)	dksA	4488813..4489268 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=990546 AAHX83_RS21735 WP_008807384.1 4484049..4484849(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-050]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990546 AAHX83_RS21735 WP_008807384.1 4484049..4484849(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-050]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425