Detailed information NTG0990523

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHX85_RS20480
• Genome accession: NZ_CP153194
• Coordinates: 4243345..4244145 (+)
• Length: 266 a.a.
• NCBI ID: WP_023292017.1
• Uniprot ID: A0A483KIE1
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain T7-072
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4238345..4249145

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHX85_RS20445 (AAHX85_20410)	gspH	4238498..4239013 (+)	516	WP_285203299.1	type II secretion system minor pseudopilin GspH	-
AAHX85_RS20450 (AAHX85_20415)	gspI	4239010..4239375 (+)	366	WP_032452553.1	type II secretion system minor pseudopilin GspI	-
AAHX85_RS20455 (AAHX85_20420)	gspJ	4239372..4239968 (+)	597	WP_285203300.1	type II secretion system minor pseudopilin GspJ	-
AAHX85_RS20460 (AAHX85_20425)	gspK	4239965..4240945 (+)	981	WP_077259115.1	type II secretion system minor pseudopilin GspK	-
AAHX85_RS20465 (AAHX85_20430)	gspL	4240938..4242134 (+)	1197	WP_048296714.1	type II secretion system protein GspL	-
AAHX85_RS20470 (AAHX85_20435)	-	4242127..4242612 (+)	486	WP_023292019.1	type II secretion system protein M	-
AAHX85_RS20475 (AAHX85_20440)	-	4242602..4243330 (+)	729	WP_023292018.1	type II secretion system protein N	-
AAHX85_RS20480 (AAHX85_20445)	pilD	4243345..4244145 (+)	801	WP_023292017.1	A24 family peptidase	Machinery gene
AAHX85_RS20485 (AAHX85_20450)	hrpB	4244193..4246622 (-)	2430	WP_044521056.1	ATP-dependent helicase HrpB	-
AAHX85_RS20490 (AAHX85_20455)	thpR	4246695..4247231 (+)	537	WP_374014337.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHX85_RS20495 (AAHX85_20460)	sfsA	4247231..4247947 (+)	717	WP_023292014.1	DNA/RNA nuclease SfsA	-
AAHX85_RS20500 (AAHX85_20465)	dksA	4248110..4248565 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29274.82 Da
• Isoelectric Point: 8.8894

>NTDB_id=990523 AAHX85_RS20480 WP_023292017.1 4243345..4244145(+) (pilD) [Klebsiella quasipneumoniae subsp. quasipneumoniae strain T7-072]
MTTLAALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDEPITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQARISWSYPLTELATGLLFILAGALLAPGLALAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQALPQVLLLASASGLAWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGVFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990523 AAHX85_RS20480 WP_023292017.1 4243345..4244145(+) (pilD) [Klebsiella quasipneumoniae subsp. quasipneumoniae strain T7-072]
ATGACGACATTAGCCGCATTATCGCTGCATTTTCCCCTTGTCTGGTATGGTTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTACCGTCTGCCGCGCATGCTGACCCAAACCGCGGACGACGAGCCGATAACCCTCAGCA
CGCCGGGCTCATCCTGCCCGCAGTGTCGCCAGCCGATAGCCTGGCGGGACAATATCCCGCTGCTCAGCTTCCTCTGGCTA
GGCCGTCGCGCCCGCTGCTGTCAGGCGCGCATCTCGTGGAGCTATCCGCTGACCGAGCTGGCTACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGGCGCTGGCGGGCGGCCTGGTGCTGCTCTCCTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACCCTGCCCCTGCTGTGGGCCGGACTGCTGTTTAATCTTAAC
GAGGTGTACATTGCGCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGCAAAGAGGCCCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGCTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCAGCGGGCTGGCGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGAGGGGGCGTTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

3D structure

• AlphaFold DB: A0A483KIE1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425