Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MasN3_RS03830 Genome accession   NZ_AP026966
Coordinates   828671..830146 (+) Length   491 a.a.
NCBI ID   WP_281912470.1    Uniprot ID   -
Organism   Massilia varians strain N-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 823671..835146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MasN3_RS03805 (MasN3_07480) - 825074..826096 (+) 1023 WP_281912460.1 SIS domain-containing protein -
  MasN3_RS03810 (MasN3_07490) - 826096..826638 (+) 543 WP_281912464.1 HAD family hydrolase -
  MasN3_RS03815 (MasN3_07500) lptC 826667..827269 (+) 603 WP_281912466.1 LPS export ABC transporter periplasmic protein LptC -
  MasN3_RS03820 (MasN3_07510) lptA 827305..827862 (+) 558 WP_281912468.1 lipopolysaccharide transport periplasmic protein LptA -
  MasN3_RS03825 (MasN3_07520) lptB 827883..828656 (+) 774 WP_281914626.1 LPS export ABC transporter ATP-binding protein -
  MasN3_RS03830 (MasN3_07530) rpoN/rpoN1 828671..830146 (+) 1476 WP_281912470.1 RNA polymerase factor sigma-54 Machinery gene
  MasN3_RS03835 (MasN3_07540) hpf 830205..830558 (+) 354 WP_281912474.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  MasN3_RS03840 (MasN3_07550) - 830711..831796 (+) 1086 WP_281912477.1 enoyl-CoA hydratase/isomerase family protein -
  MasN3_RS03845 (MasN3_07560) rapZ 831928..832806 (-) 879 WP_281912480.1 RNase adapter RapZ -
  MasN3_RS03850 (MasN3_07570) hprK 832845..833780 (-) 936 WP_036208776.1 HPr(Ser) kinase/phosphatase -
  MasN3_RS03855 (MasN3_07580) - 833905..834387 (-) 483 WP_281912483.1 PTS sugar transporter subunit IIA -

Sequence

Protein


Download         Length: 491 a.a.        Molecular weight: 54206.65 Da        Isoelectric Point: 5.1228

>NTDB_id=99051 MasN3_RS03830 WP_281912470.1 828671..830146(+) (rpoN/rpoN1) [Massilia varians strain N-3]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQEIEQILHDNPLLERLDDPLDRSVRLLADGAINTAPANGEAPPAPP
DSVAQEGAKAEHEGADGEGNGERGDAEMDWGGEGGGARSGDDEDGRPQLEADGITLREHLAEQIRLSACGMRDRALAELV
IDALDDNGYLEEPLDEIHARLPQELEIEMDELRCALALVQSLDPVGVGARSAAECLALQIRRMPGVPLVTRRLALKIVEG
YLSWFAQREYTKLKKALDCDDEDLREVQAVVRQCNPHPGAAFSSGVSDYVVPDVIVRRAKNGWQVTLNPEVMPKLRVNSL
YANLLKQGRSESQMGAQLQEAKWLIKNMRQRFDTILRVAEAIVERQRNFFSHGAVAMRPLVLREIADTLGLHESTISRVT
TQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIAQLTGAEDPRNPLSDSKIAEMLGEQGMVIARRTVAKYREAL
KIPPVSLRKSL

Nucleotide


Download         Length: 1476 bp        

>NTDB_id=99051 MasN3_RS03830 WP_281912470.1 828671..830146(+) (rpoN/rpoN1) [Massilia varians strain N-3]
ATGAAACAGTCTCTCCAGTTACGCACGTCGCAGCACCTGGCGCTCACGCCGCAGCTGCAGCAGTCGATCCGCCTGCTGCA
GCTTTCCACGCTCGAACTGCACCAGGAGATCGAGCAGATCCTGCACGACAACCCGCTGCTCGAACGGCTCGACGATCCGC
TCGACCGCTCGGTGCGGCTGCTGGCGGACGGCGCGATCAATACCGCGCCGGCCAATGGCGAAGCGCCGCCCGCCCCGCCG
GATTCGGTGGCGCAGGAGGGCGCGAAGGCCGAGCACGAGGGCGCCGATGGCGAGGGCAACGGCGAGCGCGGCGATGCCGA
GATGGACTGGGGTGGTGAAGGCGGCGGGGCGCGCAGCGGCGACGACGAAGACGGCCGCCCGCAGCTGGAAGCCGACGGCA
TCACGCTGCGCGAGCACCTGGCCGAGCAGATCCGCCTGAGCGCCTGCGGCATGCGCGACCGCGCGCTGGCCGAGCTGGTG
ATCGATGCGCTCGACGACAACGGCTACCTGGAAGAGCCGCTCGACGAGATCCATGCACGCCTGCCGCAGGAACTCGAGAT
CGAGATGGACGAACTGCGCTGCGCGCTGGCGCTGGTGCAGAGCCTGGACCCGGTGGGCGTGGGCGCGCGCAGCGCGGCCG
AATGCCTGGCCCTGCAGATCCGCCGCATGCCGGGCGTTCCCCTGGTGACGCGGCGCCTCGCCCTGAAAATCGTCGAAGGC
TATCTGAGCTGGTTCGCCCAGCGCGAATACACCAAGCTGAAAAAGGCGCTCGACTGCGACGACGAAGACCTGCGCGAGGT
GCAGGCCGTGGTCCGCCAATGCAATCCGCATCCCGGCGCGGCCTTCTCCTCGGGCGTATCGGACTACGTGGTGCCGGACG
TGATCGTGCGCCGCGCCAAGAACGGCTGGCAGGTAACGCTGAACCCGGAAGTCATGCCGAAGCTGCGCGTGAACAGCCTG
TACGCCAACCTGCTCAAGCAGGGCAGGAGCGAAAGCCAGATGGGCGCGCAACTGCAGGAAGCCAAGTGGCTGATCAAGAA
CATGCGCCAGCGCTTCGACACGATCCTGCGTGTGGCCGAGGCAATCGTCGAGCGCCAGCGCAACTTTTTCAGCCACGGCG
CGGTTGCCATGCGCCCTCTTGTTTTGCGTGAGATAGCTGATACACTAGGCCTACACGAGAGCACGATTTCACGTGTAACC
ACGCAGAAATACATGCTGACCCCGCACGGCATGTTTGAGTTGAAGTACTTCTTCGGAAGCCACGTCGCCACCGAAGCGGG
GGGAGAAGCCTCCTCGACGGCGATCCGCGCGCTTATTGCGCAATTGACAGGAGCAGAAGACCCTAGGAATCCTCTATCCG
ACAGCAAGATCGCGGAGATGCTCGGGGAACAAGGTATGGTCATTGCGCGCCGGACGGTTGCCAAATACCGCGAAGCGCTC
AAGATTCCGCCCGTCAGCCTCCGCAAATCCCTGTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-489)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(131-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

58.333

100

0.599

Multiple sequence alignment