Detailed information NTG0990321

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHY21_RS01525
• Genome accession: NZ_CP153067
• Coordinates: 324329..325129 (-)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain T7-267
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 319329..330129

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHY21_RS01505 (AAHY21_01500)	dksA	319910..320365 (-)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-
AAHY21_RS01510 (AAHY21_01505)	sfsA	320528..321244 (-)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHY21_RS01515 (AAHY21_01510)	thpR	321244..321780 (-)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHY21_RS01520 (AAHY21_01515)	hrpB	321853..324282 (+)	2430	WP_032740432.1	ATP-dependent helicase HrpB	-
AAHY21_RS01525 (AAHY21_01520)	pilD	324329..325129 (-)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAHY21_RS01530 (AAHY21_01525)	-	325126..325872 (-)	747	WP_012968922.1	type II secretion system protein N	-
AAHY21_RS01535 (AAHY21_01530)	-	325862..326347 (-)	486	WP_012968921.1	type II secretion system protein M	-
AAHY21_RS01540 (AAHY21_01535)	gspL	326340..327536 (-)	1197	WP_023339600.1	type II secretion system protein GspL	-
AAHY21_RS01545 (AAHY21_01540)	gspK	327529..328509 (-)	981	WP_023297078.1	type II secretion system minor pseudopilin GspK	-
AAHY21_RS01550 (AAHY21_01545)	gspJ	328506..329102 (-)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
AAHY21_RS01555 (AAHY21_01550)	gspI	329099..329464 (-)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHY21_RS01560 (AAHY21_01555)	gspH	329461..329976 (-)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=990321 AAHY21_RS01525 WP_008807384.1 324329..325129(-) (pilD) [Klebsiella variicola subsp. variicola strain T7-267]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990321 AAHY21_RS01525 WP_008807384.1 324329..325129(-) (pilD) [Klebsiella variicola subsp. variicola strain T7-267]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATTACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425